What is the importance of salt concentrations during the DNA extraction protocol?
In: Biology
Results table
|
Bradford (mL) |
BSA (μL) |
Absorbance |
|
3 |
0 |
0 |
|
3 |
10 |
0.228 |
|
3 |
20 |
0.402 |
|
3 |
30 |
0.553 |
|
3 |
40 |
0.715 |
|
3 |
50 |
0.776 |
|
3 |
30 |
0.58 unknown |
In: Biology
DNA replication requires many enzymes to accomplish the process successfully. This results in a replication process that is known to be bi-directional, semi-conservative and semi-discontinuous. You discover a new prokaryotic organism that lacks the gene coding for an RNA polymerase I enzyme, and therefore this enzyme is not made in this species. Additionally, the DNA polymerase III enzyme from this organism lacks any 3’ to 5’ nuclease activity but has 5’ to 3’ nuclease activity. Based on what you know about DNA replication and the functions of DNA polymerase I and DNA polymerase III, discuss the effect of these changes on DNA replication in this organism. Please explain your answer in full.
In: Biology
Please list and explain minimum 3 techniques used for gene expression analysis on mRNA level??
In: Biology
The isotypes of immunoglobulin heavy-chains
______________________________.
(Select all that apply.)
A. are determined by the heavy-chain C and V
regions
B. are gamma (γ), alpha (α), mu (μ), epsilon (ε), and delta (δ)
C. include κ and λ
D. carry out different functions in the immune response
In: Biology
Question 25 pts
The hereditary/genetic material of living cells is
Group of answer choices
RNA
DNA
Either of the above, depending on the organism
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Question 26 pts
The genetic material of viruses is
Group of answer choices
RNA
DNA
Either of the above, depending on the virus
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Question 27 pts
The process in which cells take up free DNA from their environment is called
Group of answer choices
Transcription
Transformation
Translation
Transmutation
Question 28 pts
The Hershey-Chase experiment utilized bacteriophage to demonstrate that DNA, and not protein, is the genetic material by
Group of answer choices
Transforming bacteria and subjecting mice to different bacterial strains
Labeling proteins with 32P and nucleic acids with 35S
Using a combination of nucleases and nucleic acids under different conditions
Labeling nucleic acids with 32P and proteins with 35S
In: Biology
1. Describe how the causes of sickness and disease were explained in ancient times, prior to the invention of the microscope.
2. Give two examples of foods that have historically been produced by humans with the aid of microbes.
3. What is the main difference between prokaryotes and eukaryotes?
4. Name two types of protists and two types of fungi.
5. How do viruses differ from other organisms?
In: Biology
1. In the 1970s Dr. J Robin Warren used a technique not widely used by pathologists at the time, which allowed him to make novel observations that ultimately resulted in a new treatment for ulcers. What was this technique?
Select one:
a. The use of a fistula to see inside the stomach
b. The use of a high-power microscope
c. The use of a stain for visualizing bacteria
2. Darwin's observations in the Pampas grasslands of Argentina led him to the conclusion that
Select one:
a. species change through time.
b. species change with geographical distance.
c. the Earth must be much older than was widely believed at the time.
d. climate alone cannot account for the distribution of species.
e. similar species tend to be geographically clustered.
3. Which of the following historical figures argued that species were not fixed, i.e. able to change over time into new species?
Select one:
a. Cuvier
b. Aristotle
c. Hutton
d. Lamarck
e. Linnaeus
Monarch butterflies belong to the genus Danaus, the order Lepidoptera, the class Insecta and the family Nymphalidae. All members of the family Nymphalidae have brush-like hairs on their lower legs. The same brush-like hairs will be found on the lower legs of any other member of
Select one:
a. the genus Danaus
b. the class Insecta
c. All of these
d. the order Lepidoptera
The scientific name of the dove tree is Davidia involucrata Baillon. The word "Baillon" in this name is
Select one:
a. the subspecific epithet
b. the genus
c. the authority
d. the specific epithet
In: Biology
4. In the near future, it will soon be possible for people to increase their muscle mass by introducing extra copies of the gene for insulin-like growth factor into their muscle cells.
a. What therapeutic applications can you see for such procedures? [2 marks]
b. It is possible that some people might use this procedure for cosmetic purposes—to gain muscle mass without exercise. Do you think that this would be acceptable, bearing in mind that humans already use cosmetics to change their appearance? [2 marks]
In: Biology
DNA transposons and retrotransposons are similar:
|
they both have poly-A segments at one end. |
||
|
both can encode an enzyme required for mobilization. |
||
|
transposition results in movement of the original transposon to a different place in the genome, losing the previous spot. |
||
|
during transposition, both go through an RNA form that is copied back into DNA. |
In: Biology
COVID-19 belongs to the genera Beta-coronavirus. Compare and contrast the genomic and phenotypic structure between COVID-19 and one other member of the Beta-coronavirus genera, along with their associated pathogenesis?
In: Biology
Ann O'Rexia suffers from anorexia nervosa. In addition to a low body weight, decreased muscle mass, glycogen, and fat stores, she has iron deficiency anemia. She constantly feels weak and tired. When she walks, she feels pain in her calf muscles. On this visit to her nutritionist, they discuss the vitamin content of her diet and its role in energy metabolism. She is deficient in thiamine, riboflavin, biotin and niacin (the vitamin precursor of NAD+). In the context of the metabolic pathways discussed in Chapter 14, specifically how would these affect carbohydrate metabolism.
In: Biology
Compare and contrast the three types of coronaviruses: "SARS-Cov, MERS-Cov and Covid-2019" in regards to their replication cycle, transmission, R0
In: Biology
In: Biology
Explain why no organelles, such as a nucleus, are seen in the Anabaena. (Please type it out)
In: Biology