what happen to the enzymes of an organism when is placed in an environment at a temperature higher than the maximum growth temperature? Does the organism die or only stop growing (stop cell division)? Why?
Excessive alcohol consumption has been strongly linked with
fatty liver disease
(steatosis) and individuals with defective very low density lipoprotein cholesterol
(VLDL-c) are at higher risk of developing the condition. Explain how excessive
alcohol intake can be a risk factor of steatosis and the increase risk in alcohol
consumers with defective VLDL-c.
b. Triglycerides (TG) mobilization from adipocytes is crucial
for survival during
starvation. A patient was found losing weight and showed drastic reduction of
energy (ATP) within few days of fasting. Further laboratory investigations
revealed that the patient lacks the ability to store fats, marked by high rate of TG
mobilization. Give the possible reasons for the increased lipolysis and also
explain how albumin level can be used as an indicator of lipolysis.
Please answer the following questions regarding your microbe. Please cite all of your sources.
a) What is the life cycle of disease for your microbe in humans?
b) What virulence factors does your microbe possess?
c) What is known about the regulation and function of the virulence factors in your microbe?
1) A plasmid has the antibiotic resistance genes for kanamycin and ampicillin. You insert foreign DNA into the ampicillin resistance gene. E. coli containing the recombinant plasmid (plasmid + foreign DNA) will grow in the presence of ampicillin but not in the presence of kanamycin.
2)Expression of a gene could result in...
A) transcription of a mRNA from the gene
B) transcription of a protein from the gene
C) translation of the DNA of the gene
3)Restriction enzymes like EcoRI are endonucleases. An endonuclease will...
A) Break glycosidic bonds
B) Break a phosphodiester bond only at the 3' or 5' end of DNA
C) Break a phosphodiester bond in the interior of a polynucleotide but not at the ends.
1. Describe the gene and protein E. coli beta-glucuronidase. What is its biological function?
2. Describe the pET28a plasmid.
Compared to glycerol (shown), O2 has a higher rate of passive permeability through a cell membrane. Which of these correctly explains why? Choose all that apply.
a. The most energetically challenging part of traversing a membrane is passing through the hydrophobic interior. Hydrophobic molecules can more easily do this and so can pass through membranes more easily.
b. The most energetically challenging part of traversing a membrane is passing through the polar head groups. Polar molecules can more easily do this and so can pass through membranes more easily.
c. Being composed of two identical atoms, electrons are shared equally within O2, making it non-polar, while glycerol is polar.
d. Glycerol is more hydrophobic than O2, which contains highly electronegative atoms.
e. O2 is smaller than glycerol
I am trying to isolate the proteins found in the blood of Alzheimer's patients with the hope of finding a biomarker that can potentially indicate the early onset of the disease and was wondering how I could do so? I know centrifugation is an option but how would I go about doing it specifically?
-my end goal is to simply isolate the proteins from the blood samples in order to run ITRAQ analysis on them and MS analysis after
Parent 1: AA Bb cc
Parent 2: Aa Bb Cc
A, B, and C are dominant.
How many distinct phenotypes could be produced?
Humans are bipedal, terrestrial omnivores. As a species, we are adapted to quite a range of habitats, from deserts to mountains and from rain forests to tundra. Ultimately, however, we are descended from early hominids who evolved in African plains, and our body systems tend to reflect this. Your job is to determine how humans might be adapted to specific environments if they had evolved to eat a herbivorous diet (grasses or leaves).
Write a brief description of the digestive system, and why it would look the way it does. Consider the following:
To date, which biomes have been the most heavily affected by humans? Which seems to be lightly affected? Offer a clear explanation.
3-carbon-atom (3C) molecule produced in glycolysis is called ______________Inside a eukaryotic cell, this molecule moves from ________________ to _______________ and is oxidized into _______________ . If oxygen is not present, it gets reduced into ______________ (in yeast) or ______________________ (in our muscle cells). Production of ATP in the absence of O2 is called _______________________ .
explain the effects of each of the following on the rates of glucose and/or glycogen metabolism
a) effects on decreasing the concentration of fructose-1,6-bisphosphate. support your answer by stating the biochemical principle that governs metabolic regulation
b) decreasing the amount of blood glucagon. support your answer by briefly explaining the role of glucagon.
An investigator writes that the instrument used was valid because it has a high Cronbach alpha. Is the investigator’s conclusion correct? Explain your answer. (Using
Nursing research: Reading, using, and creating evidence (4th ed.). Philadelphia: Jones & Bartlett. as a main source)