What if you wanted to test the effect of sucrose containing media on lac operon expression?

Design an experiment that would allow you to accomplish this.

1. The bacterium Micrococcus luteus lives on mammalian skin where it feeds on amino acids and lactic acid found in sweat. Answer the following questions about carbohydrate metabolism[1] in M. luteus.

1. M. luteus lacks any hexokinase orthologs (e.g. any enzyme catalyzing the reaction glucose + ATP ⇌ glucose-6-phosphate + ADP) as well as any phosphohexose isomerase homologs (e.g. any enzyme catalyzing the reaction glucose-6-phosphate ⇌ fructose-6-phosphate), but they express all other enzymes involved in glycolysis. Are M. luteus bacteria capable of catabolizing glucose via glycolysis? (1 point)

2. M. luteus expresses glycogen phosphorylase as well as phosphoglucomutase; i.e. these bacteria can convert glycogen to glucose-6-phosphate. Assuming M. luteus bacteria can transport glycogen into their cytoplasm, can M. luteus catabolize glycogen (e.g. via glycolysis) and, if so, how many ATP molecules can M. luteus produce (via glycolysis) for each 6 carbons of glycogen? (3 points)

3. Given your answer to the previous question, how many ATP molecules can an M. luteus bacterium produce (via glycolysis) from one 30,000 carbon molecule of glycogen? (2 points)

Which pathway enables M. luteus to produce sugars (e.g. for oligosaccharide biosynthesis) from glucogenic amino acids: glycolysis, gluconeogenesis, the Calvin cycle or the pentose phosphate reactions?

in response to comment: ok..so take time then

How do communication signals participate in reproductive isolation? Give one example of a signal that is species-specific. Why are some signals individually specific?

1- What is SS pharmacokinetic concept?

2- What is the rationale behind its use in clinical practice?

3- What are the equation/s used in reaching a specific plasma drug concentration using SS pharmacokinetics?

The Shh signalling pathway is important for patterning of multiple organs
during development, including the neural tube. Explain the molecular
basis of the Shh signalling pathway, and how it acts to regulate
patterning of the spinal cord. Include in your answer a brief description
of the spinal cord patterning you would expect if the Smoothened
protein were (a) completely inactive, and (b) permanently active.(500words)

With regards to Pompe disease discuss the steps an accredited
laboratory will go through to reach a definitive diagnosis. In your answer
you should consider how the laboratory maintains the quality of their
service.

Determine whether each event occurs during initiation, elongation, or termination. Initiation Elongation Termination

In prokaryotes, the shine-dalgarno sequence pairs with rRna,

Translocation occurs,

In Ecoli, EF-Tu delivers an aminoacyl-tRNA to the ribosome,

In Ecoli, mRNA binds to the 30S ribosomal subunit,

Initiator tRNA enters the P site,

In Ecoli, EF-Tu hydrolyzes GTP,

In Ecoli RF1 recognizes UAG and binds at the A site

19.1. Distinguish between poisonous organisms and venomous organisms. Give an example of each. 19-4. What kinds of toxins are produced by Aspergillus flavus, Fusarium, Trichoderma, Aspergillus, and Penicillium? Give some examples of these toxins. 19-6. What kind of organism causes “red tide”? What are some of the symptoms of poisoning by “red tide” toxins?

5) What is the observed latitudinal gradient of biodiversity? Define three hypotheses that explain the latitudinal gradient.

4. What is an Okazaki fragment and explain why it is found only in the lagging strand in a replication fork, but not the leading strand.

5. What is the role of a telomerase in eukaryotes and why are they not needed in bacterial cells?

6. Using a translation table, there is only one possible sequence of nucleotides in the template strand of DNA listed below that would code for the polypeptide sequence Phe-Leu-Ile-Val. Identify the correct one and explain for all other choices why they cannot be correct.

A) 5' UUG-CUA-CAG-UAG 3'.

B) 3' TTT-GTT-TAG-CAG 5'.

C) 5' AAG-GAA-TAA-CAA 3'.

D) 3' AAA-AAT-ATA-ACA 5'.

E) 3' AAA-GAA-TAA-CAA 5'.

1. What does it mean that DNA is an anti-parallel dimer?

2. Describe the mechanism of how a nucleotide is incorporated at the 3' end of newly synthesized DNA strand.

3. What are the roles of primase, DNA pol I and DNA pol III in the replication fork?

Diagram an adrenergic receptor. Label the ligand-binding domain. Label the transmembrane domain. Label the intracellular domain. Name a ligand for an adrenergic receptor. What happens to the adrenergic receptor when this ligand binds?

Before proceeding, you wish to know whether this gene is actually expressed in humans (your target market for therapeutic development). ?

Describe, in detail, 2 efficient experimental approaches to answer this question.

Discuss whether your proposed methods measure synthesis or accumulation of the product.