Dr. Melody is preparing 30 µg of a new ‘unknown’ Lambda digest (with OgwXI), to be used for her class. She wants to set up a slight overdigestion using 3x the standard amount of restriction enzyme in a total volume of 300 μl, and has the following reagents:

l DNA @ 2 µg / µl

molecular water

10X reaction buffer

OgwXI restriction enzyme @ 5 U / µl

However, Dr. Melody messed up. This was her protocol: 15 μl λ DNA, 30 μl reaction buffer, 37 μl molecular water, 18 μl OgwXI

Which components are correct and which are incorrect?

Do you think this reaction will be successful anyway? Why or why not?

How should the reaction setup be corrected?

Show your calculations to justify your answer.

List and describe the mechanisms used to pause the progression through the cell cycle?

30. The most effective means of sterilization is _________________________.

31. Are commercially canned foods sterile?

32. What is the most important organism to eliminate from canned foods?

33. What kind of organism stains with the Ziehl-Neelsen method?

34. What is the primary dye in the stain mentioned in the prior question?

35. What is the color of an organism that retains the primary dye?

36. Name a species that will stain with the primary dye in this stain?

37. What is the decolorizing agent in this stain?

38. What is the counterstain in this stain?

39. Name 3 uses for solid media.

when writing a standard operating procedure (SOP) for a light microscope, what is the purpose, scope, prerequisites and responsibilities ?

What is the “amplification effect” in disease ecology? Provide two real-world examples of the amplification effect.

method of collection for this specimen.

- abscess of skin

- Blood - Bone marrow

-fecees

-Genital

- Cerebrospinal fluid

- anaerobic cultures

Compare and contrast the prokaryotic and eukaryotic genomes by giving 5 similarities and 5 differences. Based on what you learned about the endosymbiont theory predict three characteristics about mitochondrial DNA.

Rosacea and Psoriasis: Rosacea affects over 16 million Americans, and over 7 million have Psoriasis. What are rosacea and psoriasis? How are they similar and different? What are the causes and the treatments for each? 

choose a topic (health issue) related to dental hygiene and discuss how this story would affect your personal life starting with an introduction explanations and conclusion and explain your position where you stand in the story ... please and thank you

Discuss the relationship between cancer and telomerase activities. Summarize the experimental evidence that suggests telomerase activities are important for cancer cells.   Discuss the role of p53 and telomerase activities are related.

Can the reults if an MIC test be used to determine whether an anitbiotic is bactericidal or bacteriastatic? If not, set up an experimental procedure to determine whether the effect is bactericidial or bacteriastatic.

What is a codon and how do you identify them in a strand of mRNA? There are 64 potential codons for only 20 amino acids. Why does this occur? Why is it also important to know the start codon before determining what a codon would be in a given strand of mRNA? After answering this first part, explain the difference between the DNA template and coding strands and explain how each strand relates to the mRNA strand that is produced?

You have been told to obtain a sputum sample and to perform microbiological staining in order to determine the identity of the pathogen causing a patient’s illness. You first perform a Gram stain, but upon microscopic analysis you visualize a mixture of pink and purple bacilli. Explain the results you have just observed and discuss what you may now do in order to identify the pathogen.

Which of the following reactions are catalyzed by kinases: