Explain why acid-fast stains are useful and why the Gram stain is not used instead?

Discuss how nuclear hormone receptors work, and the diversity of their DNA targets, or response elements, and physiological effects. Be specific.

A. In a Meselson-Stahl type experiment, bacterial cells grown in 15N medium for many generations were washed and then allowed to grow in 14N medium. DNA was extracted just after transfer (0 generation) and then after 1 and 2 generations. All these samples were analyzed by CsCl gradient centrifugation. From DNA isolated from generations 0, 1 and 2 show.

B.Position of bands in the centrifuge tubes

C. 14N and 15N labelled DNA strands

Answer both questions for each mode of replication, conservative and dispersive mode of replication.

Identify the key components of the charge master, and explain the role of the charge master in revenue cycle management.

Describe the evolution of value-based purchasing. Explain if there is a difference between value-based purchasing (VBP) and pay-for-performance (P4P).

14) Briefly Discuss the interaction of anaerobic versus aerobic ATP production during exercise.

(Please answer the question using your own words and explanation) do not use the answers that are already available on chegg or the internet) Thank you in advance!

If a trait exhibits considerable individual hybrid vigor but little maternal hybrid vigor, which is likely to perform better: a two-breed F₁population, or the corresponding two-breed F₂population?  Explain.

If a trait exhibits considerable maternal hybrid vigor but little individual hybrid vigor, which is likely to perform better: a two-breed F₁population, or the corresponding two-breed F₂population?  Explain.

That is all the information given, this is a genetics question.

Match the type of natural selection with the correct example.

a) tall giraffes and short giraffes are prominent but average height giraffes are rare

b) tall giraffes and short giraffes are rare but average height giraffes are common

c) only giraffes with very long necks survive

1.   disruptive selection

2.   stabilizing selection

3.   directional selection

Which of the following is an example of convergent evolution?

a) two populations of birds that evolved from a single species

b) two plant populations that evolved from a single plant species

c) two small animal populations that developed in different continents but share long floppy ears in common

d) two individuals that built their musculature at the same gym so they could compete against each other

Which of the following is NOT a key principle of natural selection?

a) Characteristics of organisms are inherited

b) More offspring are produced than are able to survive

c) Offspring vary among each other in regard to their characteristics

d) All offspring will survive if they help each other out during rough times

With natural selection, generally, the longer an organism lives, the more reproductive opportunities it will have. TRUE OR FALSE

With natural selection, generally, more organisms will exist with the same favorable trait than the previous generation. TRUE OR FALSE

All bacteria are harmful because they cause disease. TRUE OR FALSE

All protists are harmful because they pollute rivers and lakes. TRUE OR FALSE

Which of the following is an example of a dimorphic organism?

a) Mold growing on the surface of a food and yeast growing inside an infected person--both from the same organism.

b) Bipolar disorder in humans expressing two personality traits

c) Two appearances of a microscopic organism depending on whether you are looking a the top of the cell or the bottom

d) An organism like the butterfly that has a wormlike, larvae stage and a winged, insect stage.

1. All mutations are harmful.

a) True

b) False

2. Which of the following is TRUE about mutations?

a) Mutations are more likely to occur if they result in a useful trait

b) Mutations can be inherited.

c) Mutations only occur during DNA replication

d) Mutations do not occur in organisms that reproduce asexually

3. Which of the following would increase the chances of getting a mutation?

a) Wearing a hat outside

b) Putting sunscreen on your face and arms

c) Playing soccer at noon when the sun is strongest

d) Washing a car in the shade

Case Study 1: Meagan

Meagan is 35 years old, married with 2 children and works part time as a waitress. She does 3 gym classes per week including weights and strength training, and walks for ½ hour most days.

Although not overweight, she decided, with her gym advisor to complete a 12 week challenge to change her diet to lower her body fat percentage and become fitter and healthier.

She was given a diet by her gym advisor:

Her measurements:

Prior challenge (cm):

Chest: 85         

Arms:    24

Legs:     52

Waist:   74

Hips:     97.5

Weight: 63.8

Height: 171

Energy level: 5/10

Post challenge (cm):

Chest: 81         

Arms:    24

Legs:     49

Waist:   69

Hips:     90.5

Weight: 58.3

Height: 171

Energy Level: 9/10

Meagans comments about the 12 week challenge:

My exercise regime didn't change much, it was mainly the food and water intake that was the game changer for me. I ate pretty healthy before, but ate carbs more & later in the day as well. Portion sizes were also a problem for me prior to diet- I usually ate too much and only drank max 2ltrs water daily. I saw results in week 1 & I loved every week of this diet. Delicious, healthy meals & knowing what & when to eat helped a lot too.

Calculate her BMI; prior and post challenge

Analyse the diet by completing a food group analysis and then looking at total daily kJ, macronutrient levels and the micronutrients; B9(folate) and calcium, comparing energy, macronutrients and micronutrients to their respective RDIs. (Refer to the information sheets located below the workshop heading in this Topic (8)).

Answer the following questions:

1. What type of diet is this and what is the premise this diet is based on?

2. How much energy is supplied daily by this diet and how does this compare to the RDI for this person?

3. Which nutrients, if any are outside the RDIs in this diet and are there consequences (negative or positive) if this diet was used long term? If there are negative consequences, what health problems could result?

4. How would you rate this diet and exercise routine?

For each, whether they illustrate forward, or reverse, genetic approaches, and explain why you made that choice.

A.Your graduate student read a paper in which mutations in a specific antioxidant gene leads to resistance to the biological effects of cocaine in mice. She decides to use Cas-CRISPR to make a targeted mutation in the Drosophila homolog of this gene and examine the effects of cocaine on the resulting mutant flies. Is this a forward or reverse genetic approach?

B. Your graduate student has performed a genetic screen and isolated twenty mutations that lead to resistance to the drug PCP in flies. She proceeds to clone and sequence the mutated genes. Is this a forward or reverse genetic approach?

CONSERVATION BIOLOGY

Define the following terms using one sentence for each term: conservation biology, restoration ecology, and biodiversity (including genetic diversity, species diversity, and ecosystem diversity).

List the key benefits of biodiversity, and discuss in one short paragraph several threats to biodiversity.

Define population conservation, and explain in three to four sentences the rationale behind this approach.

Some cancers are consistently associated with the duplication of a particular part of a chromosome. What type of gene is most likely to be found in the duplicated region?

Define the term “bioconcentration” and explain in two to three sentences how certain chemicals used in pest control may affect all organisms in an ecosystem.

Using one or two sentences for each item, define each of the following environmental issues and explain the contribution of human technological advances to each: toxic waste, eutrophication, thermal pollution, smog, acid rain, the greenhouse effect, overgrazing, deforestation, erosion, and the introduction of invasive species.