Why do evergreen plants of northern wetlands have many xerophytic features? Should this also be true for deciduous plants in these habitats?

How might you distinguish between a lymphocytosis from an infection versus one from a malignancy?

Your neighbors have been feeding the birds – a lot – and now they’ve got rats in their yard, happily feasting on the leftover birdseed. You don’t want the rats near your house, and having little regard for the life of vermin, you decide to place a little “deterrent” on your property. Nosing around in a lab, you find arsenic. OK, you are smart enough not to actually use it, but you can’t help but think about it...

If a rat ingests arsenic, the enzyme __________________________ will be inhibited, and the metabolic intermediate ___________________________ will NOT be converted to ___________________________ which is the key entry point to the energy-generating pathway known as the ___________________________. Instead, this metabolic intermediate will be shunted into the metabolic pathway known as ___________________________ which does not require the function of the inhibited enzyme. The glucose- derived end-product of this pathway is ___________________________ and the net energy yield from glucose is ___________________________ NADH and ___________________________ ATP.

Can you please write about DNA extraction from blood and preparation of blood samples for DNA extraction. This is the protocol:

1.Take 750 μl of blood from each blood sample, and transfer it to new 1.5ml tube;
2. Mix 750 μl of blood with 1ml RBC lysis buffer, respectively (avoid stock contamination by not touching the walls of tube by pipette tip);
3. Centrifuge at 2300 rpm for 5 minutes;
4. Take out the liquid, keep the pellet;
5. Mix 1 ml of RBC lysis buffer with pellet in 15ml centrifuge tube;
6. Add PBS up to 14 ml by pipette controller (increase volume and contrast);
7. Centrifuge at 2200 rpm, at 4°C, for 5 minutes
8. Discard the supernatant, keep the pellet;

9. Add 1 ml of RBC lysis buffer;
10. Add PBS up to 10 ml;
11. Centrifuge at 2200 rpm, at 4°C, for 5 minutes;
12. Discard the supernatant and resuspend the pellet in 1 ml of Trizol;
13. Store the samples at -20°C

Can you explain the protocol, why are we doing these steps. Also if you can say the function of each chemical:

• RBC lysis buffer
• PBS
• Trizol

Thank you in advance. I really really need your help.

1. Arrange the following molecules from least to most specific with respect to the original nucleotide sequence: RNA, DNA, Amino Acid, Protein.

2. Identify two structural differences between DNA and RNA.

3. Suppose you are performing an experiment in which you must use heat to denature a double helix and create two single stranded pieces. Based on what you know about nucleotide bonding, do you think the nucleotides will all denature at the same time? Use scientific reasoning to explain why.

EXPERIMENT 1: CODING

1. Using the red, blue, yellow and green beads, devise and lay out a three color code (or codon) for each of the following letters (codon). For example Z = green : red : green.

• C:

• E:

• H:

• I:

• K:

• L:

• M:

• O:

• S:

• T:

• U

• Start:

• Stop:

• Space:

PLEASE LABEL WITH NUMBERS ON SIDE/ ALSO TYPE ANSWERS AS I CANT READ HANDWRITTEN ANSWERS

Describe the experimental basis for the following fundamental principle about mutations:

“The mutational process is random in the sense that whether a particular mutation happens is unrelated to any adaptive advantage it may confer on the organism in its environment. A potentially favorable mutation does not arise because the organism has a need for it.”

What are some enzymatic challenges used by plants to evolve under increasing co2 concentrations and where does each carbon cycle pathway give benefit to the plant?

Write a 500 word essay on why eliminating carcinogens from the environment would be a more successful strategy than attempting to find a cure for cancer. You can organize the essay as if it were a rhetorical (argumentative) essay.  1) Introductory paragraph that includes your point or view, three reasons that you will argue (ordered from strong to strongest), and some background, 2) Paragraph 2: argument in favor of point 1 (strong point), 3) Paragraph 3: argument in favor of point 2 (stronger point), 4) Paragraph 4: argument in favor of point 3 (strongest point), and 5) Paragraph 5: summary of argument (powerful restatement of first paragraph).

Quarantines of different kinds are imposed in pandemics. Lockdowns, shelter in place, stay at home like legal orders are invoked by the governments and are implemented with the help of law enforcement agencies (if required). Such regulations create ethical issues of privacy & its violation. Please discuss all the above in the light of Coronavirus COVID-19 pandemic. Write in no less than 1 page & no more than 2 pages

Why is cumulative incidence difficult to measure directly? Name one reason and explain why.

Compare and contrast SYBR Green versus TaqMan?

What would you expect to see if you placed a bead soaked in FGF8 on the segmental plate of a chick embryo?

Match each phrase on the left with the best term on the right. You may only use each number only once.

A 24-year-old female was hospitalized with a 10 day history of increasing fever, one or two severe shaking chills daily, and progressive weakness. A chronic, nonproductive cough, which the patient attributed to moderately heavy smoking, probably had become more prominent during the two or three weeks preceding hospitalization. A diagnosis of primary thrombocytopenia had been established approximately one year previously, based on the presence of splenomegaly. Initial physical findings included a temperature of 102 F orally, a pulse of 110 per minute, respiration’s 24 per minute, and a blood pressure of 110/70 mm Hg. The patient appeared acutely ill, dyspneic, and extremely apprehensive. Conversation was difficult because of intermittent paroxysms of coughing, which produced no sputum. Several nontender lymph nodes, up to 1 cm in diameter, were readily palpable in each axilla. The spleen was enlarged, with a firm, nontender edge descending at least 6 cm below the left costal margin on deep inspiration. Initial lab data include a total leukocyte count of 20,800 per cubic mm, a differential of 42% neutrophils, 25% band forms, and 19% lymphocytes, and a hematocrit of 42; the platelet count was 2,120,000 per cubic mm. Chest X-ray revealed a moderately dense pulmonary infiltrate extending out from the right hilum into the right lower lobe. One of the two blood cultures obtained at the time of admission to the hospital and before any antimicrobial agents were administered yielded a slow growing gram-negative bacillary rod. It was identified as Pseudomonas aeruginosa. The attending physician elected to initiate antimicrobial therapy with penicillin G administered intravenously, 2.5 million units every six hours. Because of the febrile course, with spiking fever ranging as high as 105.6 F, evidence of an increase in the right lower lobe infiltrate on a subsequent X-ray, and the report of a gram-negative bacilli in one of the two blood cultures, penicillin therapy was discontinued and cephalothin therapy was initiated. A lung biopsy of the right lower lobe was performed and the specimen revealed many focal granulomas consisting largely of histiocytes and epithelioid cells, with some areas of necrosis and caseation. Innumerable acid-fast bacilli were present.

What is the microbe?