Digest the following proteins with the proteases listed and comment whether the original sequence can be inferred based on the proteolytic patterns a. MARRIEDEARLYANDPAIDDEARLY: Separately with trypsin and chymotrypsin b. WINETHISAD: Separately with elastase and chymotrypsin. c. MYSTERYPI: With both trypsin and elastase together

Consider an LB broth flask into which two E. coli strains are inoculated. Strain "A" is wild-type, with g = 22 min at 37oC, and 45 min at 27oC. Strain "B" has a temperature-sensitive mutation, such that g = 52 min at 37oC, but at 27oC g = 44 min.We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 106 cfu/ml) into each of two separate 1 liter volumes of LB broth at time = 0. One of the flasks is incubated at 37oC, one at 27oC.Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:

1) After 6 hours of growth, what is the final concentration of each strain in each flask?

2) What fraction of the total population is Strain A and Strain B in each flask?

How can a protein act as a signal integrating sensor?

1. Explain how lipids are digested and absorbed.

2. Differentiate among saturated, monounsaturated, and polyunsaturated fatty acids in terms of structure and food sources.

3. List four classes of lipids and the role of each in nutritional health. Distinguish between fatty acids and triglycerides.

Which phase do the chromosomes duplicate?

Which phase does the cell spend most of its lifetime and approximately how long?

What is the mitotic spindle?

What is a centriole and what is its function?

Centrioles are located inside what?
Where does the mitotic spindle grow out of?

Duplicate chromosomes and duplicated centrosomes would mark the end of what?

Do chromosomes form before or after the cell enters mitosis?

How is the chromosome structured?

Sister chromatids form when the chromosome ____

Sister chromatids contain what when compared to each other?

The lifetime of a cell or time between cell divisions refers to what?

What is interphase?

What is M phase?

Suppose we know p and q for a given 2-allele population. Which term in the Hardy-Weinberg Equilibrium provides the frequency of heterozygotes in that population?

What are two species of spore-forming bacteria that cause disease and what role do the spores play in causing the disease?

Please explain

Anaphase, Metaphase, Prophase, Telephase and Interphase and what are their orders. Thank you.

The restriction enzymeneal?

Why is the liver unable to utilize acetyl-CoA for gluconeogenesis? Why is this not a limitation for plants? Which enzymes are we missing for this pathway?

Metaphors have been used in science as far back as Darwin. Pick a famous female scientist that used metaphors in her work and answer the following:

Introduce your female scientist. In what scientific discovery did this female scientist use metaphors?

Research and analyze at least three metaphors used in her work and explain what the metaphors meant. Do you think Metaphors play an important part in science and mathematics? Explain.

The following is a gene sequences in human that related to ovarian cancer. Design a pair of primers for PCR amplification of this gene using Primer 3. Print the primer 3 output, and answer the following questions:

DNA sequences

•1 ttaaaagttc actggaactg gaagaaatgg atttatctgc cgtccaaatt caagaagtac 61 aaaatgtcct tcatgctatg cagaaaatct tagagtgtcc gatctgtttg gaactgatca 121 aagaacctgt ttccacaaag tgtgaccaca tattttgcaa attttgtatg ctgaaacttc 181 ttaaccagaa gaaagggcct tcacaatgtc ctttgtgtaa gaatgagata accaaaagga 241 gcctacaggg aagcacaagg tttagtcagc ttgctgaaga gctgctgaga ataatggctg 301 cttttgagct tgacacggga atgcagctta caaatggttt tagtttttca aaaaagagaa 361 ataattcttg tgagcgtttg aatgaggagg cgtcgatcat ccagagcgtg ggctaccgga 421 accgtgtcag aaggcttccc caggtcgaac ctggaaatgc caccttgaag gacagcctag 481 gtgtccagct gtctaacctt ggaatcgtga gatcagtgaa gaaaaacagg cagacccaac 541 ctcgaaagaa atctgtctac attgaactag actctgattc ttctgaagag acagtaacta 601 agccaggtga ttgcagtgtg agagaccagg aattgttaca gaccgcccct caagaagctg 661 gagatgaagg caagctgcac tctgcagaag aggctgcttg tgagttttct gagggcataa 721 gaaacattga acatcatcaa tgcagtgatg atttaaaccc tactgagaat catgcaactg 781 aaaggcatcc agaaaaatgt cagagtattt ctatttcaaa tgtgtgtgtg gagccatgtg 841 gcacagatgc tcatgccagc tcattacagc ctgagaccag cagtttattg ctcattgaag 901 acagaatgaa tgcagaaaag gctgaattct gtaataaaag caaacagcct ggcatagcag 961 tgagccagca gagcagatgg gctgcaagta aaggaacatg taacgacagg caggttccca 1021 gcactgggga aaaggtaggt ccaaacgctg actcccttag tgatagagag aagtggactc 1081 acccgcaaag tctgtgccct gagaattctg gagctaccac cgatgttcct tggataacac 1141 taaatagcag cgttcagaaa gttaatgagt ggttttccag aactggtgaa atgttaactt 1201 ctgacagcgc atctgccagg aggcacgagt caaatgctga agcagctgtt gtgttggaag 1261 tttcaaacga agtggatggg ggttttagtt cttcaaggaa aacagactta gtaacccccg 1321 acccccatca tactttaatg tgtaaaagtg gaagagactt ctccaaacca gtagaggata 1381 atatcagtga taaaatattt gggaaatcct atcagagaaa gggaagccgc cctcacctga 1441 accatgtgac tgaaattata ggcacattta ttacagaacc acagataaca caagagcagc 1501 ccttcacaaa taaattaaaa cgtaagagaa gtacatccct tcaacctgag gacttcatca 1561 agaaagcaga ttcagcaggt gttcaaagga ctcctgacaa cataaatcag ggaactgacc 1621 taatggagcc aaatgagcaa gcagtgagta ctaccagtaa ctgtcaggag aacaaaatag

1. What is the sequences of the primers? (5’-3’)

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2.What is the size of amplification products

3.What is the length of your primer?

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4.What is your GC content of your primer (GC%)?

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In terms of the hierarchy of information place these into order for use in deriving an acceptable daily intake for a food contaminant? Explain your reasoning.

a) Scientific paper from an academic research institute on a 2 year mouse feeding study,

b) 1 month rodent feeding study conducted to good laboratory practice (GLP) standards,

c) 6 month gavage study in the dog conducted to GLP?