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In: Biology

The following is a gene sequences in human that related to ovarian cancer. Design a pair...

The following is a gene sequences in human that related to ovarian cancer. Design a pair of primers for PCR amplification of this gene using Primer 3. Print the primer 3 output, and answer the following questions:

DNA sequences

•1 ttaaaagttc actggaactg gaagaaatgg atttatctgc cgtccaaatt caagaagtac 61 aaaatgtcct tcatgctatg cagaaaatct tagagtgtcc gatctgtttg gaactgatca 121 aagaacctgt ttccacaaag tgtgaccaca tattttgcaa attttgtatg ctgaaacttc 181 ttaaccagaa gaaagggcct tcacaatgtc ctttgtgtaa gaatgagata accaaaagga 241 gcctacaggg aagcacaagg tttagtcagc ttgctgaaga gctgctgaga ataatggctg 301 cttttgagct tgacacggga atgcagctta caaatggttt tagtttttca aaaaagagaa 361 ataattcttg tgagcgtttg aatgaggagg cgtcgatcat ccagagcgtg ggctaccgga 421 accgtgtcag aaggcttccc caggtcgaac ctggaaatgc caccttgaag gacagcctag 481 gtgtccagct gtctaacctt ggaatcgtga gatcagtgaa gaaaaacagg cagacccaac 541 ctcgaaagaa atctgtctac attgaactag actctgattc ttctgaagag acagtaacta 601 agccaggtga ttgcagtgtg agagaccagg aattgttaca gaccgcccct caagaagctg 661 gagatgaagg caagctgcac tctgcagaag aggctgcttg tgagttttct gagggcataa 721 gaaacattga acatcatcaa tgcagtgatg atttaaaccc tactgagaat catgcaactg 781 aaaggcatcc agaaaaatgt cagagtattt ctatttcaaa tgtgtgtgtg gagccatgtg 841 gcacagatgc tcatgccagc tcattacagc ctgagaccag cagtttattg ctcattgaag 901 acagaatgaa tgcagaaaag gctgaattct gtaataaaag caaacagcct ggcatagcag 961 tgagccagca gagcagatgg gctgcaagta aaggaacatg taacgacagg caggttccca 1021 gcactgggga aaaggtaggt ccaaacgctg actcccttag tgatagagag aagtggactc 1081 acccgcaaag tctgtgccct gagaattctg gagctaccac cgatgttcct tggataacac 1141 taaatagcag cgttcagaaa gttaatgagt ggttttccag aactggtgaa atgttaactt 1201 ctgacagcgc atctgccagg aggcacgagt caaatgctga agcagctgtt gtgttggaag 1261 tttcaaacga agtggatggg ggttttagtt cttcaaggaa aacagactta gtaacccccg 1321 acccccatca tactttaatg tgtaaaagtg gaagagactt ctccaaacca gtagaggata 1381 atatcagtga taaaatattt gggaaatcct atcagagaaa gggaagccgc cctcacctga 1441 accatgtgac tgaaattata ggcacattta ttacagaacc acagataaca caagagcagc 1501 ccttcacaaa taaattaaaa cgtaagagaa gtacatccct tcaacctgag gacttcatca 1561 agaaagcaga ttcagcaggt gttcaaagga ctcctgacaa cataaatcag ggaactgacc 1621 taatggagcc aaatgagcaa gcagtgagta ctaccagtaa ctgtcaggag aacaaaatag

1. What is the sequences of the primers? (5’-3’)

●

2.What is the size of amplification products

3.What is the length of your primer?

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4.What is your GC content of your primer (GC%)?

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Solutions

Expert Solution

What is the size of amplification products: 1639

What is the length of your primer?

Forward primer: 21 basepairs Reverse primer : 20 base pairs

What is your GC content of your primer (GC%)

Forward primer: 47%  Reverse primer : 50%

gladiator answered 1 year ago
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