The restriction enzymeneal?

Expert Solution

Definition-

The restriction enzyme is a protein that produced from bacteria and used to cleaves DNA strand at a specific site. It is also known as a restriction endonuclease.

Types-

There are four types of restriction enzyme

Type I enzymes- They cleave the DNA at random that far from the recognition site and require ATP and S-adenosyl-L-methionine to function.
Type II enzymes – They cleave DNA at specific or close to the recognition site and require magnesium for its function.
Type III enzymes – They cut DNA at sites that not far from the recognition site and they also require ATP for its function.
Type IV enzymes – They recognize the modified methylated DNA.

Applications-

They are used to insert the desired genes into plasmid vectors during gene cloning and protein production experiments.
Used in single nucleotide polymorphisms (SNPs)
For gene analysis, digest genomic DNA by Southern blot.

Note- I didn’t hear about restriction enzymeneal before that. I think you get ur answer.

gladiator answered 1 year ago

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