A hollow fiber membrane separator with a nominal molecular weight cutoff of 100,000 is fed a solution of proteins at the rate of 250 mL min-1 . The composition of the protein solution is protein A (4 g/L, MW=20,000), protein B (7 g/L, MW=150,000), and protein C (6 g/L, MW=300,000). The filtrate flow rate is found to be 116.4 mL min-1 and the flow rate of the retentate is 133.5 mL min-1 . The concentration of protein A in the retentate is found to be 5.84 g/L. What is the concentration of protein A in the filtrate? Also, what are the concentrations of proteins B and C in the filtrate and in the retentate?

7.You want to set up a PCR reaction. The protocol suggests a 50 µL reaction volume containing 1X buffer, 200 µM dNTPs, 0.5 µM each primer (forward and reverse), 250 ng template DNA, and 1 U enzyme.

You have the following reagents: 5 X buffer, 10 mM dNTPs, 10 µM each primer (forward and reverse), 125 ng/µL template DNA, and 2 U/µL enzyme.

How many µL of water, reaction buffer, dNTPs, each primer, template DNA and enzyme would you combine to set up that reaction? (note that the order I’ve listed these components is the order you’d add them to the reaction)

When doing a lab would you expect all students to have a colony showing antibiosis? Explain.

and

What types of microbes are known to produce antibiotic compounds?

What three reasons, and solutions, for the field of view being almost too dark see the image, but one can see light from the lamp?

Wrtie an essay explaining Invasive species management restores a plant-pollinator mutualism in Hawaii.

Consider the cross: A/a; b/b; C/c; D/d; E/e x A/a; B/b; c/c; D/d; e/e

a) what proportion of the progeny will phenotypically resemble the first parent?

b) what proportion of the progeny will genotypically resemble neither parent?

Make 25 mL of a single solution containing 10 mM MOPS [MW=209 g/mole]. How many grams MOPS do you need?

The lymphatic system has several diseases and disorders that can affect it. Utilizing your book and other sources, identify at least two diseases or disorders of the lymphatic system. Briefly describe the disorders you found, their symptoms, and any other interesting information you have found.

What are the different nucleic acid monomers that are found in the cell, what types of macromolecules are they components of, and how do they base-pair? ​

How would an organism be able to survive these conditions on another planet? Please explain.

Thermodynamics - the temperature is around 233.15 K (-40 F)

Acquiring energy - receives about 70% of the sunlight that Earth receives from the Sun

Gravity - increase of gravity of 30% compared to Earth. However,more gravity means greenhouse effects increase the overall heat of the planet.

Circular orbit - seasonal changes in climate would be minimal.

Curly wing (CY) shape and stubble bristles (SB) are both recessive lethal alleles (i.e. dominant in terms of wing shape/bristles phenotype, but recessive for lethality). What would be the result (F₁ phenotypic ratios) of a cross between two curly winged, stubble bristle flies? To develop a hypothesis to predict the phenotypic ratio for the F₁ generation consider the following:

What are the genotypes of the parents? _________________

What phenotypic ratio would you predict for the offspring of a cross between two flies with curly wing shape? __________________

and two flies with stubble bristle?__________________

Use a branch diagram to predict the outcome of dihybrid cross between two curly winged, stubble bristle flies.

Describe how natural selection works in five steps or components

Female with stubble bristles crossed to a a male with stubble bristles

Info:

SB-Stubble Bristles

+ Wild Type

Female 422 SB

Male 406 SB

Female 192 +

Male 187 +

1. Draw the cross and the F1 generation

2. Predict the F1 geneotypes and phenotypic ratios

3. Use goodness of fit/apriori chi squared (X2) analysis to confirm the most likely F1 phenotypic ratio.

4. How is Stubble Bristles inherited ?

Section 29.1

A.) What distinguishes craniates from all other chordates? What distinguishes vertebrates from all other craniates?

B.) Describe the invertebrate clade of chordates, Cephalochordata. Make sure to mention the defining trait. How do they obtain their nutrients? How do they reproduce?

C.) Describe the invertebrate clade of chordates, Urochordata. Make sure to mention the defining trait. How do they obtain their nutrients? How do they reproduce?

D.) Describe the difference between agnathans and gnathostomes. Give an example of each.

What is the definition of Intelligence? How can we measure Knowledge when the term Intelligence has never been defined adequately?

Do you think we can measure knowledge based on the use of mental tests? In your opinion do you think that critical and logical thinking should be part of these tests and how has the current research in testing changed over the last ten years?