An Hfr strain that is met + pro + bio + his + was mated with an F- strain that was met - pro - bio - his -. Mating was interrupted and the percentage of cells with each phenotype was determined by replica plating the cells on minimal media plates that lacked met, pro, bio or his. The data shown in Figure 1 were obtained. (Figure 1)
Using the data in Figure 1, label each line on the results graph below.
Drag the phenotype to the appropriate line on the graph.
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Generally speaking, which of the following mutations would most severely affect the protein coded for by a gene?
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Label the four mutated DNA segments shown below according to the type of point mutation each represents. Use the codon table above to determine how each mutation would affect the amino acid coding for each segment.
Drag the labels to their appropriate locations to identify the type of point mutation shown.
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Drag each label into the appropriate bin depending on whether it applies to primary, secondary, tertiary, or quaternary structure
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The diagram below shows an mRNA molecule that encodes a protein with 202 amino acids. The start and stop codons are highlighted, and a portion of the nucleotide sequence in the early part of the molecule is shown in detail. At position 35, a single base-pair substitution in the DNA has changed that would have been a uracil (U) in the mRNA to an adenine (A).
Based on the genetic code chart below, which of the following would be the result of this single base-pair substitution?
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Practice Problem 50 A tRNA anticodon is 5' GAA 3'. Answer the following questions. |
Part A Which one of the following codons is recognized by this tRNA? Which one of the following codons is recognized by this tRNA?
Part B The first base at the 5' end of the anticodon has relaxed base-pairing rules allowing some tRNAs to bind multiple codon sequences. This is called the third base "wobble" hypothesis. How many codons could a tRNA with the anticodon 5'-GAA-3' recognize?Enter your answer as a whole number.
Part C Which amino acid does this tRNA carry?
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Compare and contrast the properties of DNA polymerase and RNA polymerase.
Drag the appropriate items to their respective bins.
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If all 5' and 3' splice sites were always used to process the pre-mRNA into mRNA, how many possible distinct mRNA sequences would result from this processing?
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In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains.
Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment first and the recipient chromosome next.
You create a lac operon partial diploid with this genotype:
F' I+ P+ Oc Z- Y+ / I- P+ O+ Z+ Y-
To determine which genes are transcribed and under what conditions, you need to first consider each genotype separately, and then together.
Part A
For each region of the lac operon on the P plasmid, I+ P+ Oc Z- Y+, determine whether the region is wild type (that is, it produces a functional protein or it's a correct protein binding sequence) or whether the region is mutated.
Part B
Use the information from Part A to determine how the operon is regulated for this genotype (I+ P+ Oc Z- Y+).
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For each region of the lac operon on the F' plasmid, I+ P+ Oc Z− Y+ , determine whether the region is wild type (that is, it produces a functional protein or it's a correct protein binding sequence) or whether the region is mutated. Select all that apply. Select all that apply. The promoter sequence is correct/functional. The repressor protein is produced. The operator sequence is correct/functional. Beta-galactosidase is produced from the lacZ gene. Permease is produced from the lacY gene.
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Which of the following occurs as a result of an abundance of tryptophan in E. coli?
A.The leader sequence is not transcribed.
B.The 5 trp genes (TrpA - TrpE) are not transcribed.
C.Stalling of the ribosome at trp codons in the leader sequence
D. The 5 trp genes (TrpA - TrpE) are transcribed, but not translated.
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