Please do not forget to write down all the names of your group members.

1. Please propose the mechanism for α−β C-C bond cleavage of G6P. Draw the mechanism clearly, give the structures of the product, and then explain why steps 2 and 3 of glycolysis are necessary.

2. If glucose is labeled at C1 carbon (the C1 carbon is tagged with radioactive 14C), where would this labeled C atom be (which carbon atom number, draw the structure and label this carbon atom) when glucose has been metabolized to:

A. DHAP

B. GAP (you should be able to figure which carbon atom is the original C1 if you draw the mechanism of step 5)

C. After the reaction with G6PD enzyme (first step in pentose phosphate pathway)

D. After the oxidative decarboxylation step in PPP

E. How many % of the total pyruvate produced via glycolysis will carry this label?

3. A. What would be the fate of glucose in cells that need ATP, but not NADPH and R5P?

B. How about cells that need a lot of NADPH, but not R5P?

C. Finally, what would be the fate of glucose if the cells need a lot of R5P, but not NADPH?

Here are some hints to help you answer Q3.

1) Remember that the reactions in PPP (with the exception of the 3 in oxidative phase) are FULLY reversible.

2) Remember that glycolysis and PPP are linked by the common metabolites: F6P and GAP.

3) Although the stoichiometry of the reactions shown in class is different: glycolysis starts from 1 glucose molecule and PPP needs 3 G6P. In reality, our cells will always have more than 1 glucose molecule. 180 g of glucose (4.5 cans of soft drink) is 6.022 x 1023 molecules of glucose. Bottom line: to answer Q3, please do not worry about stoichiometry and whether there is enough glucose in the cells or not. There is truly plenty of glucose to go to any pathways as needed.

UPDATE: Question 2 refers to question 1

[10 marks] (AdditionTable.java) Write a program that displays the following addition table. Notes: You must use a nested loop. There are spaces at the beginning of each row. 1 + 1 = 2 1 + 2 = 3 1 + 3 = 4 1 + 4 = 5 1 + 5 = 6 1 + 6 = 7 1 + 7 = 8 1 + 8 = 9 1 + 9 = 10 2 + 2 = 4 2 + 3 = 5 2 + 4 = 6 2 + 5 = 7 2 + 6 = 8 2 + 7 = 9 2 + 8 = 10 2 + 9 = 11 3 + 3 = 6 3 + 4 = 7 3 + 5 = 8 3 + 6 = 9 3 + 7 = 10 3 + 8 = 11 3 + 9 = 12 4 + 4 = 8 4 + 5 = 9 4 + 6 = 10 4 + 7 = 11 4 + 8 = 12 4 + 9 = 13 5 + 5 = 10 5 + 6 = 11 5 + 7 = 12 5 + 8 = 13 5 + 9 = 14 6 + 6 = 12 6 + 7 = 13 6 + 8 = 14 6 + 9 = 15 7 + 7 = 14 7 + 8 = 15 7 + 9 = 16 8 + 8 = 16 8 + 9 = 17 9 + 9 = 18 java programing language

The below provides information about the dataset.

Data set

Using R, build a linear regression model, logistic regression and classification model for wine quality prediction and no data partition is needed.

Which approach is best, regression or classification models? Why?

Sliding clamps are loaded onto DNA by clamp loaders to serve the critical role of coordinating various enzymes on DNA. Clamp loaders must quickly and efficiently load clamps at primer/template (p/t) junctions containing a duplex region with a free 3′OH (3′DNA), but it is unclear how clamp loaders target these sites. To measure the Escherichia coli and Saccharomyces cerevisiae clamp loader specificity toward 3′DNA, fluorescent β and PCNA clamps were used to measure clamp closing triggered by DNA substrates of differing polarity, testing the role of both the 5′phosphate (5′P) and the presence of single-stranded binding proteins (SSBs). SSBs inhibit clamp loading by both clamp loaders on the incorrect polarity of DNA (5′DNA). The 5′P groups contribute selectivity to differing degrees for the two clamp loaders, suggesting variations in the mechanism by which clamp loaders target 3′DNA. Interestingly, the χ subunit of the E. coli clamp loader is not required for SSB to inhibit clamp loading on phosphorylated 5′DNA, showing that χ·SSB interactions are dispensable. These studies highlight a common role for SSBs in directing clamp loaders to 3′DNA, as well as uncover nuances in the mechanisms by which SSBs perform this vital role.”

https://academic.oup.com/nar/article/42/16/10655/2903350

(5 points) What is a sliding clamp and what is its function?

(5 points) What is a sliding clamp loader and how does it aid in the function of the sliding clamp?

(5 points) What is the significance of a recessed 3’-end DNA, during the p/t discrimination? What data and figure from the NAR paper, suggest this significance?

(5 points) What do you think would happen if the sliding clamp could not differentiate between 3’-OH and 5’-P ends of DNA? How would this effect DNA replication?

Hand calculate , showing all work, 95% and 99% confidence intervals for μ and σ. and use the TI to obtain each output with this data 0, 0, 15, 15, 16, 18, 20, 20, 20, 25, 25, 30, 30, 30, 32, 32, 33, 33, 35, 35, 35, 35, 36, 36, 38, 38, 39, 39, 40, 40, 40

yes/no data:

out of 31, 27 said yes and 3 said no

Hand calculate, showing all work, 95% and 99% confidence intervals for p. and use the TI to obtain each output.

Cansela Corporation uses a periodic inventory system and the LIFO method to value its inventory. The company began 2018 with inventory of 6,100 units of its only product. The beginning inventory balance of $87,200 consisted of the following layers: 2,600 units at $12 per unit = $ 31,200 3,500 units at $16 per unit = 56,000 Beginning inventory $ 87,200 During the three years 2018–2020, the cost of inventory remained constant at $18 per unit. Unit purchases and sales during these years were as follows: Purchases Sales 2018 19,000 20,000 2019 25,000 27,500 2020 21,000 22,000 Required: 1. Calculate cost of goods sold for 2018, 2019, and 2020. 2. Disregarding income tax, determine the LIFO liquidation profit or loss, if any, for each of the three years. 3. Determine the effects of LIFO liquidation on cost of goods sold and net income for 2018, 2019, and 2020. Cansela’s effective income tax rate is 30%.

Describe the principles of diffusion and osmosis across cell membranes and how organisms maintain an optimal internal fluid environment. Can you please talk about:

a. the mechanisms by which freshwater paramecia rid their bodies of excess water

b. how invertebrates maintain osmotic balance and waste removal using four invertebrate types as examples: flatworms, annelid

worms, insects, and hemichordates

c. the general mechanisms of salt balance and metabolic waste removal in the kidney of a terrestrial vertebrate

d. a description of the special functions of the "multiplier effect" of mammalian kidneys and of the hormone ADH in regulating

salt and water balance

Which of the following statements is true of the functions of human resource management?

Bill Thompson is the new manager of a retail sporting goods store in Vermont that is part of a national chain. Bill, who is 25 years old, has been working for the company for four years. Before his promotion, he was the assistant manager for two years at a company store in Delaware. Last week his boss, the regional manager, briefly introduced him to the employees. The profit performance of this store is below average for its location, and Bill is looking forward to the challenge of improving profits. When he was an assistant manager, he was given mostly minor administrative duties and paperwork, so this assignment will be his first opportunity to show he can be an effective manager. The company sets the base salaries of the 20 employees who work in Bill’s store, but appraisal ratings by the store manager influence the size of an employee’s annual merit raise. These recommendations must be justified to the regional manager, especially if they are not consistent with individual and department sales. Bill can suspend or fire employees with the approval of his boss, but in practice it is difficult to do so unless the recommendation is supported by a strong case. The headquarters office sets the store layout and most prices. However, the store manager can affect store performance to a limited extent. One way is to keep the cost of employees low is by making sure they are working efficiently and not taking excessive sick days. Another way is to ensure that employees are providing a high level of customer service so that customers will return to make other purchases rather than going to a different store next time. Customer service depends on knowing the products well, being polite, providing prompt service, and making sure that inventories of popular goods are maintained so that customers can find what they want. Pay is low for this type of retail selling job, turnover is high, and it takes a few months for a new employee to learn the merchandise well enough to be helpful to customers. Thus, it is also desirable to keep competent employees satisfied enough to stay with the company. Although it is only his first week on the job, Bill believes that he has already discovered some of the problems at this store. Among the various departments in the store, the ski department has the highest potential profits during the winter, because skiing and snowboarding are popular winter sports in Vermont. At the current time the department’s sales are about average for company stores in the Northeast region, with potential for considerable improvement. On several occasions Bill noticed a line of customers waited to be served in the ski department, and he overheard some of them grumbling about how long it takes to get served. One customer said he was leaving to go to another store that didn’t make him “wait all day to have the privilege of spending hundreds of dollars on ski equipment.” Bill observed that Sally Jorgenson, the department manager, spends a lot of time socializing with her salespeople and with customers, including friends who drop in to visit and talk about ski conditions, resorts, fashions, equipment, racing, and so forth. Bill, who doesn’t ski, cannot understand what they find so interesting to talk about. He wonders why anybody in their right mind would want to spend a small fortune and risk permanent injury to hurtle down a mountain in blizzard conditions, and then stand in long lines and ride up a freezing chairlift just to do it all over again! Questions

a. How much of each type of power does Bill have at this time?

b. What influence tactics could be used in this situation to influence Sally? Explain what you would actually say to Sally in the process of using each tactic.

c. What should Bill do to improve store performance?

a) What is the difference between regression and interpolation?
b) Use least squares regression to fit a straight line to the data given in Table 1 and
calculate the y value corresponding x=3.
c) Find the Lagrange interpolating polynomial using the data given in Table 1 and
calculate the y value corresponding x=3.

Table 1

Linda is a half marathon runner. She read an article in a runner’s magazine promoting a new sports beverage that contains protein. She has always used a traditional carbohydrate-rich sports beverage, and has had great results. However, she wonders if this new product would work even better and help her run faster.
Would this new sports beverage containing protein work well for Linda?
        
How much protein should Linda be consuming before and during a half marathon?

Discuss how dividen, investment and financing interact ? (500words)

Does dividen payment affect the firm's value or the original shareholders' wealth ? Why (500words)

[1] The distribution of cholesterol levels of a population of 40-year-olds is approximately normally distributed with a mean of 220 mg/deciliter and a standard deviation of 12 mg/deciliter.

A. What is the approximate probability that a randomly selected 40-year-old from this population has a cholesterol level of more than 225 mg/deciliter? (Draw an appropriate diagram, find a z-score and indicate your calculator commands.)

B. A researcher takes a random sample of 16 people from this population and calculates the average cholesterol level.

i. What is the mean of the sampling distribution for a sample of this size? Briefly justify your answer.

ii. What is the standard deviation of the sampling distribution for a sample of this size? Briefly justify your answer.

iii. What is the shape of the sampling distribution for a sample of this size? Briefly justify your answer.

C. A researcher takes a random sample of 16 people from this population. What is the approximate probability that the sample mean cholesterol level for 16 random people will be greater than 225 mg/deciliter? (Draw an appropriate diagram, find a z-score and indicate your calculator commands.)

King City Specialty Bikes (KCSB) produces high-end bicycles. The costs to manufacture and market the bicycles at the company's volume of 2,000 units per month are shown in the following table: Unit manufacturing costs Variable costs $ 240 Fixed overhead 119 Total unit manufacturing costs $ 359 Unit nonmanufacturing costs Variable 60 Fixed 139 Total unit nonmanufacturing costs 199 Total unit costs $ 558 The company has the capacity to produce 2,000 units per month and always operates at full capacity. The bicycles sell for $600 per unit. Required: a. KCSB receives a proposal from an outside contractor who will assemble 800 of the 2,000 bicycles per month and ship them directly to KCSB’s customers as orders are received from KCSB’s sales force. KCSB would provide the materials for each bicycle, but the outside contractor would assemble, box, and ship the bicycles. The variable manufacturing costs would be reduced by 30 percent for the 800 bicycles assembled by the outside contractor. KCSB’s fixed nonmanufacturing costs would be unaffected, but its variable nonmanufacturing costs would be cut by 70 percent for these 800 units produced by the outside contractor. KCSB’s plant would operate at 60 percent of its normal level, and total fixed manufacturing costs would be cut by 15 percent. a-1. Calculate the in-house unit cost that must be compared with the quotation received from the outside contractor. Assume the payment to the outside contractor is $110. a-2. Should the proposal be accepted for a price (that is, payment to the contractor) of $110 per unit? Yes No b. Assume the same facts as in requirement (a) but assume that the idle facilities would be used to produce 80 specialty racing bicycles per month. These racing bicycles could be sold for $7,900 each, while the costs of production would be $5,500 per unit variable manufacturing cost. Variable marketing costs would be $190 per unit. Fixed nonmanufacturing and manufacturing costs would be unchanged whether the original 2,000 regular bicycles were manufactured or the mix of 1,200 regular bicycles plus 80 racing bicycles was produced. What is the total net profit/loss for the following. b-1. When the company produces and sells 2,000 units of regular bicycles per month. Assume the payment to the outside contractor is $110. b-2. When the company produces 1,200 units of regular bicycles and use the idle facilities to produce 80 specially racing bicycles per month. b-3. Should the contractor’s proposal of $110 per unit be accepted? Yes No