##### Please do not forget to write down all the names of your group members. 1. Please...

Please do not forget to write down all the names of your group members.

1. Please propose the mechanism for α−β C-C bond cleavage of G6P. Draw the mechanism clearly, give the structures of the product, and then explain why steps 2 and 3 of glycolysis are necessary.

2. If glucose is labeled at C1 carbon (the C1 carbon is tagged with radioactive 14C), where would this labeled C atom be (which carbon atom number, draw the structure and label this carbon atom) when glucose has been metabolized to:

A. DHAP

B. GAP (you should be able to figure which carbon atom is the original C1 if you draw the mechanism of step 5)

C. After the reaction with G6PD enzyme (first step in pentose phosphate pathway)

D. After the oxidative decarboxylation step in PPP

E. How many % of the total pyruvate produced via glycolysis will carry this label?

3. A. What would be the fate of glucose in cells that need ATP, but not NADPH and R5P?

B. How about cells that need a lot of NADPH, but not R5P?

C. Finally, what would be the fate of glucose if the cells need a lot of R5P, but not NADPH?

1) Remember that the reactions in PPP (with the exception of the 3 in oxidative phase) are FULLY reversible.

2) Remember that glycolysis and PPP are linked by the common metabolites: F6P and GAP.

3) Although the stoichiometry of the reactions shown in class is different: glycolysis starts from 1 glucose molecule and PPP needs 3 G6P. In reality, our cells will always have more than 1 glucose molecule. 180 g of glucose (4.5 cans of soft drink) is 6.022 x 1023 molecules of glucose. Bottom line: to answer Q3, please do not worry about stoichiometry and whether there is enough glucose in the cells or not. There is truly plenty of glucose to go to any pathways as needed.

UPDATE: Question 2 refers to question 1

In: Biology

##### [10 marks] (AdditionTable.java) Write a program that displays the following addition table. Notes: You must use...

[10 marks] (AdditionTable.java) Write a program that displays the following addition table. Notes: You must use a nested loop. There are spaces at the beginning of each row. 1 + 1 = 2 1 + 2 = 3 1 + 3 = 4 1 + 4 = 5 1 + 5 = 6 1 + 6 = 7 1 + 7 = 8 1 + 8 = 9 1 + 9 = 10 2 + 2 = 4 2 + 3 = 5 2 + 4 = 6 2 + 5 = 7 2 + 6 = 8 2 + 7 = 9 2 + 8 = 10 2 + 9 = 11 3 + 3 = 6 3 + 4 = 7 3 + 5 = 8 3 + 6 = 9 3 + 7 = 10 3 + 8 = 11 3 + 9 = 12 4 + 4 = 8 4 + 5 = 9 4 + 6 = 10 4 + 7 = 11 4 + 8 = 12 4 + 9 = 13 5 + 5 = 10 5 + 6 = 11 5 + 7 = 12 5 + 8 = 13 5 + 9 = 14 6 + 6 = 12 6 + 7 = 13 6 + 8 = 14 6 + 9 = 15 7 + 7 = 14 7 + 8 = 15 7 + 9 = 16 8 + 8 = 16 8 + 9 = 17 9 + 9 = 18 java programing language

In: Computer Science

##### The below provides information about the dataset. Input variables (based on physicochemical tests) Fixed acidity Numeric...

The below provides information about the dataset.

 Input variables (based on physicochemical tests) Fixed acidity Numeric Volatile acidity Numeric Citric acid Numeric Residual sugar Numeric Chlorides Numeric Free sulfur dioxide Numeric Total sulfur dioxide Numeric Density Numeric pH Numeric Sulphates Numeric Alcohol Numeric (%) Wine Type red or white Output variable (based on sensory data) Quality Score between 0 and 10 in ordinal

Data set

 fixed acidity volatile acidity citric acid residual sugar chlorides free sulfur dioxide total sulfur dioxide density pH sulphates alcohol winetype quality 7.4 0.7 0 1.9 0.076 11 34 0.9978 3.51 0.56 9.4 red 5 7.8 0.88 0 2.6 0.098 25 67 0.9968 3.2 0.68 9.8 red 5 7.8 0.76 0.04 2.3 0.092 15 54 0.997 3.26 0.65 9.8 red 5 11.2 0.28 0.56 1.9 0.075 17 60 0.998 3.16 0.58 9.8 red 6 7.4 0.7 0 1.9 0.076 11 34 0.9978 3.51 0.56 9.4 red 5 7.4 0.66 0 1.8 0.075 13 40 0.9978 3.51 0.56 9.4 red 5 7.9 0.6 0.06 1.6 0.069 15 59 0.9964 3.3 0.46 9.4 red 5 7.3 0.65 0 1.2 0.065 15 21 0.9946 3.39 0.47 10 red 7 7.8 0.58 0.02 2 0.073 9 18 0.9968 3.36 0.57 9.5 red 7 7.5 0.5 0.36 6.1 0.071 17 102 0.9978 3.35 0.8 10.5 red 5

Using R, build a linear regression model, logistic regression and classification model for wine quality prediction and no data partition is needed.

Which approach is best, regression or classification models? Why?

In: Statistics and Probability

##### Sliding clamps are loaded onto DNA by clamp loaders to serve the critical role of coordinating...

(5 points) What is a sliding clamp and what is its function?

(5 points) What is a sliding clamp loader and how does it aid in the function of the sliding clamp?

(5 points) What is the significance of a recessed 3’-end DNA, during the p/t discrimination? What data and figure from the NAR paper, suggest this significance?

(5 points) What do you think would happen if the sliding clamp could not differentiate between 3’-OH and 5’-P ends of DNA? How would this effect DNA replication?

In: Biology

##### Hand calculate , showing all work, 95% and 99% confidence intervals for μ and σ. and...

Hand calculate , showing all work, 95% and 99% confidence intervals for μ and σ. and use the TI to obtain each output with this data 0, 0, 15, 15, 16, 18, 20, 20, 20, 25, 25, 30, 30, 30, 32, 32, 33, 33, 35, 35, 35, 35, 36, 36, 38, 38, 39, 39, 40, 40, 40

yes/no data:

out of 31, 27 said yes and 3 said no

Hand calculate, showing all work, 95% and 99% confidence intervals for p. and use the TI to obtain each output.

In: Statistics and Probability

##### Cansela Corporation uses a periodic inventory system and the LIFO method to value its inventory. The...

Cansela Corporation uses a periodic inventory system and the LIFO method to value its inventory. The company began 2018 with inventory of 6,100 units of its only product. The beginning inventory balance of $87,200 consisted of the following layers: 2,600 units at$12 per unit = $31,200 3,500 units at$16 per unit = 56,000 Beginning inventory $87,200 During the three years 2018–2020, the cost of inventory remained constant at$18 per unit. Unit purchases and sales during these years were as follows: Purchases Sales 2018 19,000 20,000 2019 25,000 27,500 2020 21,000 22,000 Required: 1. Calculate cost of goods sold for 2018, 2019, and 2020. 2. Disregarding income tax, determine the LIFO liquidation profit or loss, if any, for each of the three years. 3. Determine the effects of LIFO liquidation on cost of goods sold and net income for 2018, 2019, and 2020. Cansela’s effective income tax rate is 30%.

In: Accounting

##### Describe the principles of diffusion and osmosis across cell membranes and how organisms maintain an optimal...

Describe the principles of diffusion and osmosis across cell membranes and how organisms maintain an optimal internal fluid environment. Can you please talk about:

a. the mechanisms by which freshwater paramecia rid their bodies of excess water

b. how invertebrates maintain osmotic balance and waste removal using four invertebrate types as examples: flatworms, annelid

worms, insects, and hemichordates

c. the general mechanisms of salt balance and metabolic waste removal in the kidney of a terrestrial vertebrate

d. a description of the special functions of the "multiplier effect" of mammalian kidneys and of the hormone ADH in regulating

salt and water balance

In: Biology

##### Which of the following statements is true of the functions of human resource management?

Which of the following statements is true of the functions of human resource management?

In: Operations Management

##### Bill Thompson is the new manager of a retail sporting goods store in Vermont that is...

Bill Thompson is the new manager of a retail sporting goods store in Vermont that is part of a national chain. Bill, who is 25 years old, has been working for the company for four years. Before his promotion, he was the assistant manager for two years at a company store in Delaware. Last week his boss, the regional manager, briefly introduced him to the employees. The profit performance of this store is below average for its location, and Bill is looking forward to the challenge of improving profits. When he was an assistant manager, he was given mostly minor administrative duties and paperwork, so this assignment will be his first opportunity to show he can be an effective manager. The company sets the base salaries of the 20 employees who work in Bill’s store, but appraisal ratings by the store manager influence the size of an employee’s annual merit raise. These recommendations must be justified to the regional manager, especially if they are not consistent with individual and department sales. Bill can suspend or fire employees with the approval of his boss, but in practice it is difficult to do so unless the recommendation is supported by a strong case. The headquarters office sets the store layout and most prices. However, the store manager can affect store performance to a limited extent. One way is to keep the cost of employees low is by making sure they are working efficiently and not taking excessive sick days. Another way is to ensure that employees are providing a high level of customer service so that customers will return to make other purchases rather than going to a different store next time. Customer service depends on knowing the products well, being polite, providing prompt service, and making sure that inventories of popular goods are maintained so that customers can find what they want. Pay is low for this type of retail selling job, turnover is high, and it takes a few months for a new employee to learn the merchandise well enough to be helpful to customers. Thus, it is also desirable to keep competent employees satisfied enough to stay with the company. Although it is only his first week on the job, Bill believes that he has already discovered some of the problems at this store. Among the various departments in the store, the ski department has the highest potential profits during the winter, because skiing and snowboarding are popular winter sports in Vermont. At the current time the department’s sales are about average for company stores in the Northeast region, with potential for considerable improvement. On several occasions Bill noticed a line of customers waited to be served in the ski department, and he overheard some of them grumbling about how long it takes to get served. One customer said he was leaving to go to another store that didn’t make him “wait all day to have the privilege of spending hundreds of dollars on ski equipment.” Bill observed that Sally Jorgenson, the department manager, spends a lot of time socializing with her salespeople and with customers, including friends who drop in to visit and talk about ski conditions, resorts, fashions, equipment, racing, and so forth. Bill, who doesn’t ski, cannot understand what they find so interesting to talk about. He wonders why anybody in their right mind would want to spend a small fortune and risk permanent injury to hurtle down a mountain in blizzard conditions, and then stand in long lines and ride up a freezing chairlift just to do it all over again! Questions

a. How much of each type of power does Bill have at this time?

b. What influence tactics could be used in this situation to influence Sally? Explain what you would actually say to Sally in the process of using each tactic.

c. What should Bill do to improve store performance?

In: Accounting

##### a) What is the difference between regression and interpolation? b) Use least squares regression to fit...

a) What is the difference between regression and interpolation?
b) Use least squares regression to fit a straight line to the data given in Table 1 and
calculate the y value corresponding x=3.
c) Find the Lagrange interpolating polynomial using the data given in Table 1 and
calculate the y value corresponding x=3.

Table 1

 x 0 2 4 6 y 5 6 3 8

##### Diego Company manufactures one product that is sold for $75 per unit in two geographic regions—the...  Diego Company manufactures one product that is sold for$75 per unit in two geographic regions—the East and West regions. The following information pertains to the company’s first year of operations in which it produced 46,000 units and sold 42,000 units.
 Variable costs per unit: Manufacturing: Direct materials $25 Direct labor$ 20 Variable manufacturing overhead $2 Variable selling and administrative$ 4 Fixed costs per year: Fixed manufacturing overhead $644,000 Fixed selling and administrative expenses$ 388,000