A woman is heterozygous for X-linked disease (Hemophilia A). If she marries a man without hemophilia, is there a chance that her daughters will suffer from the disease?(choose the best option)

a.only daughters can suffer from the disease

  b.both daughters and sons can suffer from the disease

  c.none of the children will suffer from the disease

  d.only children can suffer from the disease

1. In CRISPR, why does it make sense to design our crRNA first before making any other changes to the genomic DNA?

2. In CRISPR, when searching for PAMs, GGNGG makes a much better PAM than NGG - why is this?

 A female with Muppetrus bristle mates with a male with Rheingoldenbach body color,
producing the following counts:

F1 Generation
Males
WT-WT   Disease-WT      WT-Disease      Disease-Disease
260     252             0               0

Females
WT-WT   Disease-WT      WT-Disease      Disease-Disease
258     255             0               0

I have determined that at locus 1, the mode of inheritance is sex linked and at locus 2, the mode of inheritance is autosomal recessive.

Could someone help me determine the expected number of individuals that will have the following phenotypes, and fill out the E column.

Chi Square test:

how can gel electrophoresis be used to determine whether a ligation experiment was successful? Be specific with what you would expect to see in the various lanes of the gel if a ladder, comtrol ( digested plasmid + insert without ligase) and sample ( digested plasmid + insert with ligase) were run.

What basic lessons were learned in the classic experiments about gene regulation and regulation of the even-skipped gene by CRMs that determine stripe two formation in Drosophila embryos?

. Describe the V(D)J recombination process in terms of the sequence of events that occurs and the enzymes involved at each step (4 points). Describe why V(D)J recombination is crucial for adaptive immunity

how would loop dilution, spread plate, and streak plate isolation techniques be affective by the use of selective medium

Write Personal SWOT Analysis but it should be link it to lab technician job

Read each question carefully. Write your response in the space provided for each part of each question. Answers must be written out in paragraph form. Outlines, bulleted lists, or diagrams alone are not acceptable and will not be scored.

Enolase is an enzyme that catalyzes one reaction in glycolysis in all organisms that carry out this process. The amino acid sequence of enolase is similar but not identical in the organisms. Researchers purified enolase from  Saccharomyces cerevisiae, a single-celled eukaryotic yeast that grows best at  37°C, and from  Chloroflexus  aurantiacus, a bacterium that grows best at the much higher temperature of  55°C. The researchers compared the activity of purified enolase from the two organisms by measuring the rate of the reaction in the presence of varying concentrations of substrate and a constant amount of each enzyme at both  37°C and  55°C.

(a) Depending on the organism, the optimal  pH for enolase to catalyze its reaction is between  6.5 and  8.0.  Describe how a  pH below or above this range is likely to affect enolase and its catalytic ability.

(b)  Identify the appropriate negative control the researchers most likely used when measuring the reaction rate in the presence of each organism’s enolase.

(c) The researchers predict that for any particular concentration of substrate, the  C. aurantiacus enolase-catalyzed reaction is more rapid at  55°C than at  37°C. Provide reasoning to  justify the researchers’ prediction.

Give and explain one example of each of the following types of evidence supporting evolution:
Molecular (biochemical) evidence

Anatomical evidence

Embryological evidence

Biogeographical evidence

Fossil evidence

Explain the physiological mechanism of diffusion of oxygen, how could you measure the diffusing capacity of the patient, how it might be affected by the disease, and which conditions from the vital signs may be affecting even more the already impaired mechanism of diffusion  

Haemoglobin is an amazing molecule, as it allows vertebrates to transport gases from the environment to the tissues. To boot, blood changes its oxygen-binding characteristics in response to the animal’s external AND internal environment. How does this happen? What are the advantages of this “shape-shifting” ability? How does O2 affinity of Hb compare between mammals adapted to hypoxia and those in normoxia? How does it compare between sedentary and athletic species? Explain why. Some Antarctic fish lack haemoglobin completely. Their blood is composed of plasma and a few white blood cells. How are these fishes able to survive and succeed in their environment? Explain.

A) The formation of DNA from individual nucleotides is best described as:

1. a process that will have a net yield of energy.

2. a process that will have no effect on disorder in the universe.

3. an anabolic reaction.

4. a catabolic reaction.

5. a spontaneous reaction

B)In a C4 plant where does the Calvin cycle predominantly take place?

1. in the vascular tissue.

2. in every cell in the plant.

3. in the bundle sheath cells of leaves.

4. in the mesophyll cells or leaves.

5 in the roots.

C)The formation of a polysaccharide from monosaccharides is best described as:

1. a process that will have a net yield of energy.

2. a process that will not increase disorder in the universe.

D) Chloroplast ATP synthase is present in which membrane?

1. the thylakoid membrane.

2. the mitochondrial inner membrane.

3. the chloroplast outer membrane.

4. the mitochondrial outer membrane.

5. the cristae

3. an anabolic reaction.

4. a catabolic reaction.

5. a spontaneous reaction.

In class we reviewed that effective selection of resources has implications BEYOND an individual organism’s immediate survival. What does this mean?