how can gel electrophoresis be used to determine whether a ligation experiment was successful? Be specific...

how can gel electrophoresis be used to determine whether a ligation experiment was successful? Be specific with what you would expect to see in the various lanes of the gel if a ladder, comtrol ( digested plasmid + insert without ligase) and sample ( digested plasmid + insert with ligase) were run.

Expert Solution

gladiator answered 1 year ago

How can DNA extraction, PCR, and gel electrophoresis be used in forensic science? Explain

Provide an overview of how gel electrophoresis can be used to analyse genes after treatment with...

Provide an overview of how gel electrophoresis can be used to analyse genes after treatment with restriction enzymes or amplification using PCR.

Compare and contrast native gel electrophoresis and denaturing gel electrophoresis. Which method would be used to...

Compare and contrast native gel electrophoresis and denaturing gel electrophoresis. Which method would be used to determine the molecular weight of a protein, why?

Explain the importance of how Polymerase Chain Reaction, Gel Electrophoresis and Restrictions Enzymes can be used...

Explain the importance of how Polymerase Chain Reaction, Gel Electrophoresis and Restrictions Enzymes can be used in the real world.

briefly describe how PCR and gel electrophoresis can be used to analyse DNA samples from crime...

briefly describe how PCR and gel electrophoresis can be used to analyse DNA samples from crime scenes.

1. gel electrophoresis can be used to seperate molecules by a. size b. length c. charge...

1. gel electrophoresis can be used to seperate molecules by a. size b. length c. charge d. all of the above 2. List at least 2 differences between DNA and RNA 3. If one DNA strand has the followng sequnce, what is the sequence of the sister strand of DNA? ATGTCCAGT 4. What is DNA isolation? a. the rupturing of the DNA molecule b. the moving of DNA from one cell to another c. the extraction of DNA from viruses...

Which of the following statement is FALSE? A. Gel electrophoresis is used to separate DNA according...

Which of the following statement is FALSE? A. Gel electrophoresis is used to separate DNA according to their size B. Chloroform is used for DNA precipitation C. PCR amplifies DNA to millions of copies D. Introns code for non-coding sequence whereas exons code for coding sequences Genotype frequency of the Alu insert in a population could be A. homozygotes (-/-) and homozygotes (+/+) B. heterozygous (+/-) and mongoloid population C. homozygotes (+/+) and asian population D. homozygotes (+/+) and mongoloid...

Gel electrophoresis is a process used in laboratories to separate macro molecules. Mark the statements that...

Gel electrophoresis is a process used in laboratories to separate macro molecules. Mark the statements that are true as related to gel electrophoresis. An electrical charge is applied to the gel plate The pieces of DNA move due to electrical charge and also size A sample of the DNA is placed in a small well and a charge is applied The pieces of DNA or bands are stained to be visible Running a "gel" can be used to compare a...

How can PCR and gel electrophoresis tell if individuals from different populations are related. What else...

How can PCR and gel electrophoresis tell if individuals from different populations are related. What else can these tell you about the collected DNA samples

Explain how gel electrophoresis of DNA samples works and why it was performed.

Question