11. A researcher is interested in isolating an auxotrophic mutant in the laboratory that is unable to synthesize the amino acid histadine. Explain how this researcher would have to use negative selection to isolate and identify a histadine mutant.
    

An individual has the chromosomes below where the dot represents the centromere: NOP.QRSTU NRQ.POSTU If a crossover event occurs between the O and P loci, what is the shortest chromosome formed? Enter your answer with no spaces between the loci and use a period as the centromere.

Select one of the United Nations' (U.N.) Millennial Development Goals (MDGs). Visit the website and report on the UN's progress in achieving this goal? How will meeting this goal positively impact the health of the world? What suggestions would you make to help the UN achieve this goal?

11. Is the lac operon a repressible or an inducible operon? What happens in the absence of lactose? What happens in the presence of lactose? Is the trp operon a repressible or an inducible operon? What happens in the absence of tryptophan? What happens in the presence of tryptophan?

Please answer these question briefly. Dont have to write a lot. like 2-3 sentences or less as long as get answer the questions. Please answer all thanks

1) Aspects of protein translational regulation that are key for protein folding

2) Embryonic stem cells

     a)Derivation (from fertilized eggs that would be thrown away normally)

3) What is “Pluripotent” and what are some main criteria for pluripotency

4) What are applications of pluripotent cells?

5) What are some problems with pluripotent cells?

Phylum Porifera -what kind of body symmetry do they have - what are the unique structures (why are they in this phylum not another) -how do they get their food -where are they found -common examples

please be DETAILED with the answers! this is a ruminant nutrition question do not answer if unsure

1. A complete dairy diet contains 55% alfalfa, 40% corn and 5% cotton seed meal.

• List all of the chemical compounds in the diet (e.g. starch).
• For each compound you have listed, describe the fermentation process in the rumen.
• For each compound identify what % is fermented in the rumen and what % is digested in the intestine

Pretranscriptional, Transcriptional, RNA Processing, RNA Transport,Translational, and posttranslational.

Choose 1 of the above levels of gene regulation in Eukaryotes and explain how it functions in gene regulation of eukaryotic gene expression.

5. List the most important enzyme and the inputs and outputs of the Calvin cycle. 6. Describe the three main stages of the Calvin Cycle.

5. Describe how the electrochemical gradient generated during the electron- transport chain changes the conformation of the ATP Synthase, enabling it to catalyze the covalent attachment of Pi to ADP to form ATP.

5. Define the following terms, and identify which of these four nutritional groups most of the medically important microbes would belong to.
   

   a. photoautotroph
   b. photoheterotroph c. chemoautotroph d. chemoheterotroph

Transcription factor binding sites. There are a
number of software programs that can scan a
nucleotide sequence for responsive elements. Use
one of these online software programs to scan the many responsive elements that may bind to the
transcription factor YY1 exists in this region?

Describe each of the following stains, and indicate when it is appropriate to use each one:

1. simple stain

2. differential stain

3. negative stain

4. acid-fast stain

5. flagella stain

A grad student has a culture of E. coli with a total volume of 600 mL, and OD reading at 550 nm of 1.7. He makes serial dilutions of the culture, starting with a transfer of 1 mL culture into 9 mL tryptone broth. Next, he transfers 0.1 mL of this dilution into 0.9 mL tryptone broth, and repeats this two more times to make a total of 4 serial dilutions. He then pipets 200 uL from the fourth dilution on an agar plate, spreads it over the surface, and puts the plate in an incubator to grow overnight. The next day, he counts 152 cfus (colony forming units) on the plate.

How many viable cells are present in the 600 mL starting culture?

Answer is 4,560,000,000 but need to show work.