Describe how you would identify genes responsible for transcription in E. coli by isolating mutations in their genes. Remember that these genes are required for cell viability. Include all the steps in the isolation of the mutants and also describe how you will differentiate the mutants you want from those in other genes necessary for viability. This includes giving me the expected result for mutants in the genes you want.

The average sperm count in males is now lower than it was several decades ago. The reasons for lower sperm counts observed in males today is not known. Propose a hypothesis as to why, global, sperm counts are lower today than then were fifty years ago. What experiment could you do to test your hypothesis?

what is the function of HSPGs in the stem cell niche in your own words

You are studying a bacterium that grows in a particular ecological niche. You cannot culture it in the laboratory, but you can isolate small quantities of cells that microscopic analysis indicates are not contaminated with other bacteria. How will you obtain the ribosomal RNA gene sequence data to study the taxonomy of the bacterium? •How will you determine the complete genome sequence of the bacterium?

The steps performed for determining the complete genomics sequence of the bacterium include- Step 1: Cloning Step 2: Restriction Mapping Step 3: Gel Electrophoresis Step 4: DNA Sequence Analysis

Gel electrophoresis- The DNA fragments after Restriction digestion are separated by electrophoresis, a process that involves application of an electric field to cause the DNA fragments to migrate into an agarose gel. The gel is then stained with a methylene blue stain to visualize the DNA bands and may be photographed. Although methylene blue dye is not as sensitive as ethidium bromide it may be used to stain the higher quantities of DNA that are used in this experiment. The movement of the fragments will always be towards the positive electrode because DNA is a negatively charged molecule. The fragments move through the gel at a rate that is determined by their size and shape, with the smallest moving the fastest.

DNA Sequence Analysis- This method uses dideoxynucleotide triphosphates(ddNTPs) whichwhich an H on the 3’ carbon of the ribose sugar instead of the normal OH found in deoxynucleotide triphosphates (dNTPs). Dideoxynucleotides are chain terminators. In a synthesis reaction, if a dideoxynucleotide is added instead of the normal deoxynucleotide, the synthesis stops at that point because the 3’OH necessary for thetaddition of the next nucleotide is absent. The sequencing gel is able to resolve fragments that differ in size from each other by only one base.

**I need explain exactly gel ectrphoresis and DNA sequence analysis will help find the bacteria genome

Explain in detail type I and III hypersensitivity

what is one way the Corona virus pandemic will end?  Explain using these topics: what is a virus, vaccination, social distancing, SIR model of how diseases spread, mutation

If DNA from an evidence sample and DNA from a suspect or victim share a profile that has a low frequency in the population, this suggests that the two DNA samples came from the same person; the lower the frequency, the stronger the evidence. But the possibility remains that the match is only apparent—that an error has occurred and the true profile of one of the sources differs from that reported by the laboratory. Please discuss the ways that laboratory errors, particularly errors that might falsely incriminate a suspect, can arise, how their occurrence might be minimized, and how to take into account the fact that the error rate can never be reduced to zero.

Please discuss the quality assurance and control.

Explain/Breakdown the Chemotaxis response mechanism and their role in flagella movement and regulation. Bacterial Motility(Motor) Kit Please provide an in depth answer in its simplest form for easy understanding. Thank you

Design a microscopy experiment (widefield) testing the effect of Vertiporfin on cell death (step by step). What do we look for? which colour are we running, how long are we running it for?

Note: don't worry about the microscope, just design an experiment for staining of cells so it can be looked under widefield microscope

19. Pick the correct answer.

In Drosophila, red eyes (E) are dominant over white (e) and normal wings (W) are dominant over shriveled wings (w). Use the addition and multiplication rules to calculate the phenotypic ratios of the cross of Eeww and EeWw.

1. Cross of Ee x Ee:

2. Cross of ww x Ww:

3. Red eyes and normal wings:

4. Red eyes and shriveled wings:

5. White eyes and normal wings:

6. White eyes and shriveled wings:

Louise is a 20-year-old woman who has been admitted to an acute mental health inpatient ward following an episode of self harming behavior.this is her first contact with mental health services. Louise presents with lacerations to both arms; some are superficial scratches, others, while of varying severity and depth, are not life-threatening. There are also visible scars on her arms from apparently previous incidents of self-harming behaviors.

Louise has been admitted via the local accident and emergency department following an initial CRISIS assessment by the hospital Mental Health Liaison Team. Louise had consumed half a bottle of vodka and also admitted to smoking cannabis prior to the incident. She denies having taken an overdose of any type and this is corroborated by normal blood test results. she was originally accompanied there by her mother who did not remain with her.

Louise does not appear remorseful for what she has done. She says she is depressed and angry with her parents who 'think she is worthless and do not care about me because  i haven't gone to university like my brothers'. She maintains that she doesn't need any help with her problems, claiming that they are down to 'my immaturity, as my parents keep telling me' and that I'll grow out of it with time.

Pathology

Define the patient's disease process

Describe the patient's disease process

Clearly explain etiology of the disease process

Clearly explain the cause of the disease process

Thoroughly explain signs and symptoms of pathology

explain what is meant by the following statement:

“All viruses mutate.  However, a mutated virus is not necessarily more dangerous.”

Include answers to the following questions:

What is a mutation?

Are most mutations helpful or harmful to a virus?  Why?

What would make a virus “more dangerous” to humans?

How is Bactoprenol involved in peptidoglycan synthesis?

Please provide an in depth answer in its simplest form for easy understanding. Thank you

Part A

Please pick one behavioral, biomedical, or psychosocial treatment method. In thinking about this method, what are 2 benefits and drawbacks? Please be sure to be detailed in your discussion. (Using: Treatment method for Alcohol Abuse).

Part B

Find a scholarly research article which discusses the effectiveness of the treatment method you chose to discuss in Part A of this forum. What was the population under study? were the methods used, results, and practical implications of this study? Do you think the results of this study would be effective for all population? Why or why not? Would another treatment have worked better for the population under study? Why or why not? Please do not discuss vaccinations for opioid addiction, or the nicotine patch.