Question

In: Biology

19. Pick the correct answer. When a gene of interest has been inserted into a plasmid...

19. Pick the correct answer.

When a gene of interest has been inserted into a plasmid vector, the result is called _____ DNA.

homologous

analogous
recombinant
heterologous
restriction fragment
Restriction sites are often DNA _____, which means that both DNA strands constituting the restriction site have the same DNA sequence but in _____ orientation.
donors; parallel
palindromes; parallel
palindromes; antiparallel
vectors; antiparallel
hybrids; parallel

The restriction enzyme XYZ-1 cuts DNA in one strand of a double-stranded DNA molecule at the palindromic sequence 5′-GAATTC-3′. What is the sequence of the opposite strand also cleaved by this restriction enzyme?

3′-GAATTC-5′
3′-CTTAAG-5′
3′-GTTAAC-5′
3′-CAATTG-5′
3′-GAAAAG-5′
Name the key function of mitosis.
to replicate DNA within a cell
to generate two daughter cells genetically identical to the parent cell
to generate two daughter cells that through recombination are genetically similar to (but not identical) to the parent cell
to manufacture protein
to condense the DNA
Name two key functions of meiosis.
double the DNA content per cell in the production of gametes and properly segregate the chromosomes into each of the four sperm or egg cells formed per parent cell
halve the DNA content per cell in the production of gametes and properly segregate the chromosomes into each of the four sperm or egg cells formed per parent cell
halve the DNA content per cell in the production of somatic cells and properly segregate the chromosomes into each of the two daughter cells formed per parent cell
halve the RNA content per cell in the production of gametes and permit crossing over to occur
double the DNA content per cell in the production of gametes and permit crossing over to occur
In an attempt to simplify meiosis for the benefit of students, mad scientists develop a way of preventing premeiotic S phase and making do with having just one division, including pairing, crossing over, and segregation. Would the products of such a system differ from those of typical meiosis? Why?
The products would be identical to typical meiosis.
It would not work because the DNA needs to replicate in order for meiosis to function properly.
Such a system would generate two cells instead of four.
Such a system would not be able to provide genetically diverse gametes.
Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as mitosis proceeds. What is the number of nuclear DNA molecules present in a human cell in metaphase of mitosis?
92 DNA molecules per cell. Found as 92 physically separate chromosomes.
92 DNA molecules per cell. Found as 46 chromosomes each with two chromatids attached at the centromere.
46 DNA molecules per cell. Found as 23 chromosomes, each with two chromatids attached at the centromere.
23 DNA molecules per cell. Found as 23 chromosomes in each of two nuclei.

23 DNA molecules per cell or a haploid genetic composition.

Four of the following events are part of both meiosis and mitosis, but only one is meiotic. Which one?
chromatid formation
chromosome movement to poles
chromosome condensation
synapsis
spindle formation
Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as meiosis I proceeds. What is the number of nuclear DNA molecules present in the human cells formed at the completion of meiosis I?
46 DNA molecules per cell. Found as 46 physically separate chromosomes in each new cell.
92 DNA molecules per cell. Found as 46 chromosomes each with two chromatids attached at the centromere.
46 DNA molecules per cell. Found as 23 chromosomes, each with two chromatids attached at the centromere.
23 DNA molecules per cell. Found as 23 chromosomes in each newly formed cell.
The newly formed cells will have variable numbers of chromosomes due to genetic recombination.

Solutions

Expert Solution

Answer:

19. Pick the correct answer.

1) When a gene of interest has been inserted into a plasmid vector, the result is called recombinant DNA.

2) Restriction sites are often DNA palindromes; , which means that both DNA strands constituting the restriction site have the same DNA sequence but in antiparallel orientation.

3) The restriction enzyme XYZ-1 cuts DNA in one strand of a double-stranded DNA molecule at the palindromic sequence 5′-GAATTC-3′. What is the sequence of the opposite strand also cleaved by this restriction enzyme3?

  • 3′-CTTAAG-5′

4) Name the key function of mitosis.

  • to generate two daughter cells genetically identical to the parent cell

5) Name two key functions of meiosis.

  • halve the DNA content per cell in the production of gametes and properly segregate the chromosomes into each of the four sperm or egg cells formed per parent cell

6) In an attempt to simplify meiosis for the benefit of students, mad scientists develop a way of preventing premeiotic S phase and making do with having just one division, including pairing, crossing over, and segregation. Would the products of such a system differ from those of typical meiosis? Why?

  • Such a system would generate two cells instead of four

7) Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as mitosis proceeds. What is the number of nuclear DNA molecules present in a human cell in metaphase of mitosis?

  • 92 DNA molecules per cell. Found as 46 chromosomes each with two chromatids attached at the centromere.

8) Four of the following events are part of both meiosis and mitosis, but only one is meiotic. Which one?

  • Synapsis

9) Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as meiosis I proceeds. What is the number of nuclear DNA molecules present in the human cells formed at the completion of meiosis I?

  • 46 DNA molecules per cell. Found as 23 chromosomes, each with two chromatids attached at the centromere.

Related Solutions

19) When an extra base has been inserted (added) into a gene, the mutation is particularly...
19) When an extra base has been inserted (added) into a gene, the mutation is particularly harmful because all of the bases (and codons) after the insertion are wrong. This type of mutation is called A. silent B. nonsense C. frameshift D. aneuploidy 20) When a mutation leads to a substitution of a different amino acid within a protein, this is a _________________ mutation. A. nonsense B. missense C. silent D. deletion 21) Which of the following is true about...
You are inserting your gene of interest into the Lac Z gene in a plasmid also...
You are inserting your gene of interest into the Lac Z gene in a plasmid also containing a tetracycline resistant gene. You plate your transformed bacteria on media containing tetracyline and X-gal. Which of the following results would indicate a clone with recombinant plasmids? Select one: a. A blue colony on the tetracyline plates b. A clone which did not grow on the tetracycline plates c. A red colony on the tetracyline plates d. A white colony on the tetracyline...
Describe in detail how to clone your “gene of interest” into a bacterial plasmid using EcoRI...
Describe in detail how to clone your “gene of interest” into a bacterial plasmid using EcoRI restriction enzyme and how to generate a large quantity of the DNA using competent E. coli. Why do you use competent bacteria for this?
pBR322 is a plasmid. It has two antibiotic resistance genes a the amp gene encoding the...
pBR322 is a plasmid. It has two antibiotic resistance genes a the amp gene encoding the ampicillin resistance protein and the gene yet encoding the tetracycline resistance protein. In the plasmid, there is only one EcoRV restriction enzyme recognition sequence within the Tet gene. One EcoRI restriction enzyme recognition sequence between Tet gene and amp gene. Is amp gene a regulated gene or nonregulated gene. justify
Which of the following is/are correct regarding interest rates? Pick all the correct ones. Borrowers’ preference...
Which of the following is/are correct regarding interest rates? Pick all the correct ones. Borrowers’ preference for long duration and lenders’ preference for short duration causes the term premium to be positive. Bonds with greater default risk typically trade at lower yield-to-maturities. An inverted yield curve serves as a negative indicator for the future state of the economy. Zero-coupon bonds are less sensitive to interest rate changes compared to coupon bonds with the same time to maturity. The yield curve...
The human gene for a protein hormone has been isolated. Scientists want to introduce this gene...
The human gene for a protein hormone has been isolated. Scientists want to introduce this gene into a bacterial host, modifying the bacteria so that it can be used to produce this hormone. After successfully introducing the gene on a plasmid, the bacteria produced the protein hormone, but the protein was defective, longer than normal, with intervening stretches of amino acids not found in the native protein. Which solution would be most likely to resolve this problem? Multiple Choice A....
When comparing replication and transcription, pick the best answer that describes this property:
When comparing replication and transcription, pick the best answer that describes this property:the process ends when the polymerizing complex synthesizes a region that contains a stem-loop regionA.replication onlyB.transcription onlyC.neitherD.both
Based on the Huntington disease which has the gene of HTT gene. Answer the following questions:...
Based on the Huntington disease which has the gene of HTT gene. Answer the following questions: 1a) Is the gene exonic or not?, 1b) what  type of variant (SNP of CNV) and if the resultant changes in protein or protein level. 2) Is the variant found in the non-coding region and also is it involved in transcriptional regulation, if so describe the proposed pathway. 3) Draw a three-generation pedigree with at least 15 family members. 4) A narrative describing the pedigree...
Based on the Huntington disease which has the gene of HTT gene. Answer the following questions:...
Based on the Huntington disease which has the gene of HTT gene. Answer the following questions: 1)Description of genomic/epigenomic/cytogenetic/transcriptomic/proteomic characterization/detection of disease (how is the genetic cause determined) 2) Give a flow chart for determination of the genetic cause at the clinical level (i.e. phenomic upon the first presentation to the clinic to confirmation of genetic disorder).
Which of the following statements is NOT CORRECT? a. When a bond's coupon interest rate is...
Which of the following statements is NOT CORRECT? a. When a bond's coupon interest rate is less than the market interest rate, the bond will sell at a value less than its par value and is known as a discount bond. b. Bonds are basically loans, so they usually make regular interest payments to the bondholders. The percentage of this interest payment relative to the bond's face value is called the coupon interest rate. c. For a fixed-rate bond, the...
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT