In: Biology
19. Pick the correct answer.
When a gene of interest has been inserted into a plasmid vector, the result is called _____ DNA. | |||||||||||
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Restriction sites are often DNA _____, which means that both DNA strands constituting the restriction site have the same DNA sequence but in _____ orientation. | |||||||||||
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The restriction enzyme XYZ-1 cuts DNA in one strand of a double-stranded DNA molecule at the palindromic sequence 5′-GAATTC-3′. What is the sequence of the opposite strand also cleaved by this restriction enzyme? |
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Answer:
19. Pick the correct answer.
1) When a gene of interest has been inserted into a plasmid vector, the result is called recombinant DNA.
2) Restriction sites are often DNA palindromes; , which means that both DNA strands constituting the restriction site have the same DNA sequence but in antiparallel orientation.
3) The restriction enzyme XYZ-1 cuts DNA in one strand of a double-stranded DNA molecule at the palindromic sequence 5′-GAATTC-3′. What is the sequence of the opposite strand also cleaved by this restriction enzyme3?
4) Name the key function of mitosis.
5) Name two key functions of meiosis.
6) In an attempt to simplify meiosis for the benefit of students, mad scientists develop a way of preventing premeiotic S phase and making do with having just one division, including pairing, crossing over, and segregation. Would the products of such a system differ from those of typical meiosis? Why?
7) Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as mitosis proceeds. What is the number of nuclear DNA molecules present in a human cell in metaphase of mitosis?
8) Four of the following events are part of both meiosis and mitosis, but only one is meiotic. Which one?
9) Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as meiosis I proceeds. What is the number of nuclear DNA molecules present in the human cells formed at the completion of meiosis I?