Question

In: Biology

19. Pick the correct answer. When a gene of interest has been inserted into a plasmid...

19. Pick the correct answer.

When a gene of interest has been inserted into a plasmid vector, the result is called _____ DNA.

homologous

analogous
recombinant
heterologous
restriction fragment
Restriction sites are often DNA _____, which means that both DNA strands constituting the restriction site have the same DNA sequence but in _____ orientation.
donors; parallel
palindromes; parallel
palindromes; antiparallel
vectors; antiparallel
hybrids; parallel

The restriction enzyme XYZ-1 cuts DNA in one strand of a double-stranded DNA molecule at the palindromic sequence 5′-GAATTC-3′. What is the sequence of the opposite strand also cleaved by this restriction enzyme?

3′-GAATTC-5′
3′-CTTAAG-5′
3′-GTTAAC-5′
3′-CAATTG-5′
3′-GAAAAG-5′
Name the key function of mitosis.
to replicate DNA within a cell
to generate two daughter cells genetically identical to the parent cell
to generate two daughter cells that through recombination are genetically similar to (but not identical) to the parent cell
to manufacture protein
to condense the DNA
Name two key functions of meiosis.
double the DNA content per cell in the production of gametes and properly segregate the chromosomes into each of the four sperm or egg cells formed per parent cell
halve the DNA content per cell in the production of gametes and properly segregate the chromosomes into each of the four sperm or egg cells formed per parent cell
halve the DNA content per cell in the production of somatic cells and properly segregate the chromosomes into each of the two daughter cells formed per parent cell
halve the RNA content per cell in the production of gametes and permit crossing over to occur
double the DNA content per cell in the production of gametes and permit crossing over to occur
In an attempt to simplify meiosis for the benefit of students, mad scientists develop a way of preventing premeiotic S phase and making do with having just one division, including pairing, crossing over, and segregation. Would the products of such a system differ from those of typical meiosis? Why?
The products would be identical to typical meiosis.
It would not work because the DNA needs to replicate in order for meiosis to function properly.
Such a system would generate two cells instead of four.
Such a system would not be able to provide genetically diverse gametes.
Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as mitosis proceeds. What is the number of nuclear DNA molecules present in a human cell in metaphase of mitosis?
92 DNA molecules per cell. Found as 92 physically separate chromosomes.
92 DNA molecules per cell. Found as 46 chromosomes each with two chromatids attached at the centromere.
46 DNA molecules per cell. Found as 23 chromosomes, each with two chromatids attached at the centromere.
23 DNA molecules per cell. Found as 23 chromosomes in each of two nuclei.

23 DNA molecules per cell or a haploid genetic composition.

Four of the following events are part of both meiosis and mitosis, but only one is meiotic. Which one?
chromatid formation
chromosome movement to poles
chromosome condensation
synapsis
spindle formation
Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as meiosis I proceeds. What is the number of nuclear DNA molecules present in the human cells formed at the completion of meiosis I?
46 DNA molecules per cell. Found as 46 physically separate chromosomes in each new cell.
92 DNA molecules per cell. Found as 46 chromosomes each with two chromatids attached at the centromere.
46 DNA molecules per cell. Found as 23 chromosomes, each with two chromatids attached at the centromere.
23 DNA molecules per cell. Found as 23 chromosomes in each newly formed cell.
The newly formed cells will have variable numbers of chromosomes due to genetic recombination.

Solutions

Expert Solution

Answer:

19. Pick the correct answer.

1) When a gene of interest has been inserted into a plasmid vector, the result is called recombinant DNA.

2) Restriction sites are often DNA palindromes; , which means that both DNA strands constituting the restriction site have the same DNA sequence but in antiparallel orientation.

3) The restriction enzyme XYZ-1 cuts DNA in one strand of a double-stranded DNA molecule at the palindromic sequence 5′-GAATTC-3′. What is the sequence of the opposite strand also cleaved by this restriction enzyme3?

  • 3′-CTTAAG-5′

4) Name the key function of mitosis.

  • to generate two daughter cells genetically identical to the parent cell

5) Name two key functions of meiosis.

  • halve the DNA content per cell in the production of gametes and properly segregate the chromosomes into each of the four sperm or egg cells formed per parent cell

6) In an attempt to simplify meiosis for the benefit of students, mad scientists develop a way of preventing premeiotic S phase and making do with having just one division, including pairing, crossing over, and segregation. Would the products of such a system differ from those of typical meiosis? Why?

  • Such a system would generate two cells instead of four

7) Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as mitosis proceeds. What is the number of nuclear DNA molecules present in a human cell in metaphase of mitosis?

  • 92 DNA molecules per cell. Found as 46 chromosomes each with two chromatids attached at the centromere.

8) Four of the following events are part of both meiosis and mitosis, but only one is meiotic. Which one?

  • Synapsis

9) Human cells normally have 46 chromosomes. Let us define the transition from one cell to two cells as meiosis I proceeds. What is the number of nuclear DNA molecules present in the human cells formed at the completion of meiosis I?

  • 46 DNA molecules per cell. Found as 23 chromosomes, each with two chromatids attached at the centromere.

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