By gel electrophoresis, how do you know if your protein binded to the column? How would you modify the purification next time to make it more efficient?

describe a typical unreplicated human chromosome from tip to tip

Calculate the amount of reagent and water required to make the following solutions. Do not assume you have a stock solution unless instructed to do so.

a) 1.00 L of 20.00mM Tris-HCl, 50.0mM NaCl, 20.00mM MgSO₄ x 7H₂O, 5.00mM CaCl₂

b) 275 mL of 25% Na₂HCO₃, 18.0% EtOH

c) 750.0mL of 0.2M sodium citrate, 10.00mM SDS (from 0.50M stock), 2.5% glycerol

d) Calculate molarity of 15% NaCl and 27% hydrochloric acid

Based on what you know about anole morphology and the process of adaptation via natural selection, do you think it is possible for a generalist anole to be as adept as each of the specialist anole ecomorphs at living (for example: climbing, running, hunting, etc.) in the different island habitats? In other words, do you think it is possible for a single anole species to be as good at living on twigs as the twig ecomorph anoles, and as good at living on trunks as the trunk ecomorph anoles, and as good at living on the tops of trees as the trunk-crown ecomorph anoles, etc.?

List the three variations of carbon-fixation reactions (C3, C4, and CAM) and write their basic characteristics (locations or times of C4 and C3 pathway in each) with one or two examples for each.

What sets the limits on the stimuli that an animal can sense? What limits the movements an organism makes? How do sensory and motor systems compare across different types of animals?

1. Draw a 4 AA residue generic polypeptide with R groups (R, no specific AA required) - label clearly.

a. the peptide bond

b. one pair of H bond donor and H bond acceptor

c. outline one amide plane

d. label phi and psi for one junction

e. using your drawing, explain why peptide bonds are nearly always trans

g. label the amino terminus and carboxy terminus

2. What information does the Ramachandran plot provide and what are its implications for protein folding?

a) What is a sensory system designed to do?

b) What is the relationship between sensation and perception? What is the role of experience in creating this relationship?

What do illusions tell us about sensory processing? What is the role of proprioception in control of the body?

The 2018 Physical Activity Guidelines for Americans include all of the following recommendations for adults EXCEPT which one:

engage in moderate intensity activities for at least 150 minutes a week

engage in brief sessions of moderate to vigorous for at least 10 minutes at a time for any health benefits

engage in muscle strengthening activities at least 2 days a week

engage in vigorous activities for at least 75 minutes a week

A)Some individuals can suffer from medium-chain acyl-CoA dehydrogenase (MCAD) deficiency. Which intermediates accumulate in individuals with MCAD deficiency? Can other intermediates still be oxidized and if so, why?