In: Biology
To know the complementary DNA of a particular DNA sequence one must know the sequence of the template DNA.
Template DNA is the atrand of DNA which is used as a base for the replication. According to that ,the complementary DNA is prepared in an antiparallel manner.
Adenine (A) pairs with Thymine (T) in DNA. But in RNA there is no T, so the Adenine pairs with Uracil(U). Guanine (G) pairs with Cytosine (C) in both DNA and RNA.
For example:
5'ATGCAACCTAGCATGC3'(TEMPLATE DNA)
3'TACGTTGGATCGTACG5'(complementary strand)
3'UACGUUGGAUCGUAGC5'(RNA strand of template strand)
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