Given the sequence below 5’ AACTTCGGCTTAAATGGAGGCCAT’ What is the complementary DNA strand? What would the mRNA...

Given the sequence below

5’ AACTTCGGCTTAAATGGAGGCCAT’

What is the complementary DNA strand?
What would the mRNA strand look like?
What would the protein look like?
Create a point mutation in the DNA and then give the mRNA and protein sequence.
Create a frameshift mutation in the DNA and then give the mRNA and protein sequence.

Solutions

Expert Solution

a. 3' TTGAAGCCGAATTTACCTCCGGTA 5'

b. 5' AACUUCGGCUUAAAUGGAGGCCAU 3'

c. Asn - Phe - Gly - Leu - Asn - Gly - Gly - His

d. Mutation type - Silent point mutation

Template - AACTTTGGCTTAAATGGAGGCCAT

Complementary strand - TTGAAACCGAATTTACCTCCGGTA

mRNA - AACUUUGGCUUAAAUGGAGGCCAU

Protein - Asn - Phe -Gly - Leu - Asn - Gly - Gly - His

e. Template - AACTTTGGCTTAAATGGAGGCCAT

mRNA - AACUUUGGCUUAAAUGGAGGCCAU

Protein - Thr - Leu - Ala - Stop - Met - Glu - Ala

gladiator answered 1 year ago

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