1. Compare and contrast the digestion of proteins and lipids. Include at least two similarities and two differences.

PREPARATION OF COMPETENT CELLS and TRANSFORMATION:

2a. We will be preparing competent MM294 (E. coli) cells using a chemical treatment of CaCl₂. What are MM294 cells, why do we use them? Define “competent” cells and describe why/how CaCl₂ makes cells “competent”. Why is “mid-log” phase important? Support your drawing with a cartoon of what cells “look like” before and after treatment.

2b. What does “heat-shock” do (in the transformation procedure)?

2c. What is LB? What does “LB” stand for and what is its role in cloning? What would happen if LB was NOT on the plates?

how does the coronavirus replicate its genome and expresses its genes?

How come coronaviruses are able to mutate so rapidly?

Assume that you have recently been hired as the special assistant to the chief executive officer (CEO) of your health care organization, Thunder Hospital. Your duty is to head up the new quality improvement department. Over the last year, the hospital has experienced substantial growth but is also facing a number of patient safety concerns, including a steady increase in medical errors and a 25% rise in hospital-acquired infections. Based upon what you have learned in this course, prepare a quality improvement plan to present to the CEO with strategies for addressing these issues.

In a 1,000-1,250-word proposal, include the following:

1. Identify the issues the hospital is currently facing and how they are affecting quality outcomes and endangering patients.
2. Present a detailed plan to improve quality and elaborate on how it aligns with the hospital’s initiatives to improve value-added health care. Discuss the quality improvement tool you suggest using to locate and ameliorate problem areas, as well as the roles and responsibilities of involved stakeholders, financial considerations for the implementation of your proposal, the goals of the plan, and methods for evaluating its success.
3. Examine the effects that the implementation of this proposal will have upon administrators, clinicians, and physicians. Explore possible challenges that could arise with stakeholders reacting negatively to changes presented by this proposed plan. What strategies or preventive measures could be put in place to reduce the friction between various health care providers?
4. How will your plan improve overall quality for Thunder Hospital? How will the improvements your plan suggests implementing now set the hospital up to continue providing quality care in the future?

53. Females are likely to mate with more than one male when

Select one:

a. resources can�t be monopolized, as in robins who eat worms.

b. only some males are able to get good territories.

c. predation on eggs or on young is high.

d. the male stays with the young and/or the eggs.

e. both c and d

54. In many species, females are certain of their parenthood; males are not. Eggs and live births require a larger investment of energy than do sperm. Therefore,

Select one:

a. Male success is more dependent than is female success on the number of times he mates.

b. Female success is more dependent than is male success on the number of times she mates.

55. In many species, females are certain of their parenthood; males are not. Eggs and live births require are larger investment of energy than do sperm. Therefore,

Select one:

a. Females tend to be more competitive; males tend to be more choosey.

b. Males tend to be more competitive; females tend to be more choosey.

c. Female competition results in weaponry.

1) Define and compare the four levels of protein organization. How does the sequence of nucleotides of the gene relate to the levels of protein organization?

2) Explain transcriptional control of gene expression using the expression of the lac operon in Escherichia coli

Where are each of our macronutrients (carbohydrates,proteinsand fats) chemically digested? Where does the process for each of them begin? Also note all locations in the GI tract that these processes continue (until each macronutrient has been broken down into components that our cells can absorb).

• What ultimately characterizes different cells from each other? What makes neurons different from immune cells vs. kidney cells, etc.?