NucGreen stains dying cells that have compromised plasma membranes. What could cause false positives?

Skin color in Bulbasaurs?

There are two alleles for skin color in Bulbasaurs (diploid organisms). Bulbasaur ‘Vector’ is homozygous recessive and he has marine blue skin. Bulbasaur ‘Aria’ is heterozygous (having green and marine blue alleles), but green is dominant and so she has green skin. They have a cute son ‘Kim’ with marine blue skin. Kim mates with a female Bulbasaur who is heterozygous and they have a litter of 28 baby bulbasaurs.

1)) Draw two Punnett squares: (1) for Vector and Aria, and (2) for their son and his mate

2) How many (quantity, not percentage) of Vector’s grandchildren would we expect to have green skin? (Show all work)

Provide 4 reasons why students should study infant reflexes?

In an HIV Test using indirect ELISA,
(a) explain the principle of the assay
(b) what is not included in the negative control for indirect ELISA when testing for HIV?

Do these organisms all share the same genetic code? Explain.

(Aquatic Algae, Protist, Elephant, Caterpillar, Flower, Mushroom, Human)

Name and briefly describe the three most popular chromatography methods for shotgun proteomics (e.g. size exclusion followed by normal phase)?

In blood coagulation, there are many enzymes involved in a cascade of activation reactions to ultimately form fibrin. There is an inherited disorder in Factor X for a small subgroup of the population (bleeding disorder). It is hypothesized that 50% of this different Factor X is defective in the activation site that allows conversion to Factor Xa. You are charged with developing a pharmaceutical (drug) or biopharmaceutical (biologic) to improve this bleeding disorder.

1. Describe what protein or system you will target for drug or biologic design.  

how to write a short report on topic : Parts of flower.

Choose one antibiotic that you would expect to show different effects on Gram-positive and Gram-negative bacteria. Which bacterium is more susceptible? Why do you think this might be the case?

Researchers investigating cave fish found a new population in a previously undiscovered cave. These fish also lacked eyes, but different from the other populations, their eye development appeared to fail during lens induction. The researchers observed a failure to induce a lens placode. Their next goal was to determine the mechanism that caused loss of eyes in this population.

a) (2 points) The researchers hypothesized that a loss of function mutation in one or more of six possible genes was likely the cause. List the six most likely candidate genes.

b) (3 pts) How could the researchers determine if one of those six genes had a mutation.

c) (5 pts) Assume that the researchers found a mutation in one of those six genes. Describe an experiment the researchers could conduct using the surface fish as a model to determine if the identified mutation was sufficient to cause a failure of eye formation. List the possible results and highlight/underline the result that supports their hypothesis.

What evidence supports the conclusion that GDF11 promotes the maintenance of adult neural stem cells?

Enzyme A is required to produce red pigment on the abdomen of a bird. A biochemist evaluated birds with red and pale (not red) abdomens and determined that a minimum of 50 units of Enzyme A is necessary to obtain red pigmentation. A new mutation in the gene that produces Enzyme A, results in only 10 units of Enzyme A in birds homozygous for the mutation. The mutation would be best considered as"

A: suppressor

B: null

C: co-dominant

D: gain of function

E: leaky

Action of metabolic processes:

Oligomycin - Binds and blocks the proton channel in ATP synthase

Carbon monoxide - Inhibits cytochrome oxidase

2,4-dinitrophenol (DNP) - Binds protons and moves them down their electrochemical gradient

Rotenone - Blocks electron transfer at NADH dehydrogenase

Explain how these metabolic poisons affect different processes during cell respiration?

- Effect on electron transport (increased, decreased, no effect) and why

- Effect on ATP synthesis (increased, decreased, no effect) and why