7.You want to set up a PCR reaction.
The protocol suggests a 50 µL reaction volume containing 1X buffer,
200 µM dNTPs, 0.5 µM each primer (forward and reverse), 250 ng
template DNA, and 1 U enzyme.
You have the following reagents: 5 X
buffer, 10 mM dNTPs, 10 µM each primer (forward and reverse), 125
ng/µL template DNA, and 2 U/µL enzyme.
How many µL of water, reaction
buffer, dNTPs, each primer, template DNA and enzyme would you
combine...