chromosome test

How would you prepare adherence cells (tissue culture) in a flow cytometry?

Cells that accumulate misfolded proteins initiate a stress response that includes reducing protein translation by inhibiting the initiation stage of translation. First explain the three stages of initiation of protein translation in eukaryotic cells. Next explain the mechanism by which translation is inhibited as part of the stress response.

The three stages are 43S preinitiation complex, 48S initiation complex, and 80S initiation complex but I don't know how to explain them and what happens during each stage.

The 3 bacteria below each has different virulence factors/ mechanisms that allow them to counter the immune system. Briefly describe each case.

(1) Stahylococcus aureus

(2) Listeria monocytogenes

(3) Neisseria meningitidis

Complete the following questions. Illustrates the process of DNA transcription, translation, and protein synthesis.

1.    The stages of transcription are initiation, elongation, and termination. Draw a representation of each of these stages . Be sure to include the names of important enzymes and locations.

2.    Once mRNA is created through transcription, it is often processed. Explain how mRNA can be processed. Include the names of important enzymes or structures.

3.    Translation is how mRNA gets used to create the peptide sequence. Draw what is going on inside a ribosome. Be sure to include the locations of mRNA, tRNA, each subunit of the ribosome, and where the amino acid sequence forms.     

write10 to 15 slides with Graphs and diagrams and avoid full slide writing about this topic:

1-Genetic effect of radiation

-Definition of genetic effects
-Difference between genetic and somatic effect
-What are these genetic effects
-Table where you give the effects and the corresponding dose

1. Which of the following are ultimate decomposers?

   		bacteria
   		fungi
   		millipedes
   		Collembolans
   		mites
   		earthworms
   		Only A and B.
   		C, D, E, and F are all reducer decomposers. So are vultures!

2. Which of the following are reducer decomposers?

   		bacteria
   		fungi
   		millipedes
   		Collembolans
   		mites
   		earthworms
   		Only A and B.
   		C, D, E, and F are all reducer decomposers. So are vultures! You want to conduct an experiment on decomposition in the temperate deciduous forest. Which of the following would be usable variables for your experiment? Mesh size in litter bags. Placement of a banana peel into several different biomes. Designing a lab experiment where you drop leaves into hydrochloric and nitric acids. Both A and B. Both B and C. Both A and C.

Using model materials to demonstrate DNA replication

1.    Present a detailed analysis of DNA replication at one replication fork. Use drawing, descriptions, and/or captions detailing the process.

2.    In the analysis include the following:

a.    Show how the leading and lagging strands are synthesized

b.    Show the proteins (enzymes) involved in DNA replication and what their functions are

2.  Which vaccine is a toxoid?

3.What contributes to the diversity in the Influenza virus?

4. Mutations that occur in the Influenza virus contribute to:

5. Exchange of genes between Influenza viruses contribute to:

6. Which is a thymus independent antigen?

7. Which is a mechanism of how tumor evade the immune system:

8. Binding of PD-L1 from the tumor cell to the PD1 receptor on T cell results in:

9. CarT cells are being used as:

10. To produce CarT cells, the original T cells were isolated from other donors.

a.True

b.False

You are working in the ED at Harborview Medical Center. You are seeing a 52-year-old male patient who reports cough, fever, sweating, pleuritic chest pain and general malaise. Your examination reveals a fever of 102F, respiration rate 24bpm, rhonchi and decreased breath sounds, tachycardia with pulse of 116bpm, a tender one-inch mass on his right chest wall near his shoulder and right eye injection with reported pain and normal vision.
Your history indicates that he returned from Hawaii yesterday where he participated in the Ironman competition. He was seen in the ED in Kona, Hawaii two days earlier for similar symptoms that initiated 12 hours prior to that visit. He had an appendectomy and shoulder surgery in the past and reports episodic rashes over the past few months. He is married with adult children and works at the Fred Hutchinson Cancer Research Center in a molecular genetics laboratory with no patient care responsibilities. List two questions that you would ask the patient. For each, note your rationale (i.e. note why you think that question will help you diagnose this patients disease).

In the E. coli trp operon, what would be the effect of a deletion in the region 2 sequence, which would prevent it from forming stable RNA hairpin structure with either regions 1 or 3?

A) The Trp repressor could not bind to the operator and trp transcription will be high, even in the presence of high tryptophan concentration.
B) Translation of the leader sequence could not be initiated.
C) No effect on trp operon transcription.
D) Transcription of the trp operon could not be initiated.
E) Hairpin 3:4 would form even when tryptophan is low, and this would constitutively attenuate transcription of the Trp operon.

How would you make E.coli bacteria cells produce human insulin for you? More than one answer can be correct

Describe the life cycle of Rhizopus stolonifer. Include the Class it belongs to and the structures found in their sexual and asexual stages. Name the four (include Fungi imperfecti) classes of fungi we dealt with in the laboratory and describe how these fungi are classified. Include the Latin definition for each of the classes named.

1. A researcher is interested in elucidating the function of a gene found in humans and has chosen a mouse model. Of what relevance would comparative genomics, BLAST, and synteny be to the researcher?

2. Spemann Organizer and the primitive knot. What is their relevance and how do they differ?