How would the yield of a PCR reaction be affected by the following mistakes?

Explain your answer.

1. You forgot to add template DNA

2. you added TWICE as much template DNA as is called for by the intructions

3. You forgot to add Taq polymerase

4. You add HALF as much Taq polymerase as is called for the by instructions

Are protozoans presenting contractile vacuoles (also called pulsatile vacuoles) more commonly found in fresh or in salt water?

Are protozoans presenting contractile vacuoles (also called pulsatile vacuoles) more commonly found in fresh or in salt water?

How do amoebae, paramecia and trichomonas move?

What is the typical reproduction cycle of a DNA virus?

What characteristics of protozoa make them resemble animals?

What are retroviruses? How do they reproduce and what is the role of the enzyme reverse transcriptase?

What is the typical reproduction cycle of a DNA virus?

What is the basic structure of the HIV virus? What is the function of the glycoproteins in its envelope?

What is the basic structure of the HIV virus? What is the function of the glycoproteins in its envelope?

What is the basic structure of the HIV virus? What is the function of the glycoproteins in its envelope?

What are some mechanisms by which pathogenic bacteria cause diseases? Why is this knowledge important?

Describe a pathological condition/ disease that results from a defect in post-translational modification of proteins -----> PLEASE TYPE 250 WORDS & INCLUDE 1 - 2 REFERENCES, NO DIAGRAMS.

What are some mechanisms by which pathogenic bacteria cause diseases? Why is this knowledge important?

Mary performs a PCR reaction and runs it on an agarose gel, using 100-bp ladder as her molecular weight standard. She images the gel using UV light and sees NO bands (no band in her PCR reaction and no bands for her ladder). Which of the following could explain these results? Explain each answer.

1. Mary forgot to add Gel Red when pouring the gel.

2. Mary's PCR product had an unusually high % of G and C bases.

3. Mary connected the electrodes to her gel incorrectly, swapping the positive and negative poles.