The following chart show the results of cloning nuclei from several cell lines (e.g. v6.5, v18.6, etc.) derived from two different genetic strains of mice (F1 and 129). Note: your answers require no prior knowledge of these strains or the specific cell lines listed)

1. What is the main conclusion that can be drawn based on the results of these data with respect to success of the cloning experiments?
2. Give three possible cell intrinsic differences between v6.5 cells and LJG-13 cells that could play a role in their different results in this assay.

For one of the three potential factors you mention above, describe a brief experimental plan to test whether this factor is responsible for the differences between v6.5 cells and LJG-13 cells in this assay. Include in this plan roughly how you would perform the experiment, what your readout would be and what you could conclude based on your results.

hello I need a gene that produces an enzyme that can be easily assayed for activity.

please give me at least three choices

thanks

What is β-Carotene? Why is β-Carotene important? How is β-Carotene metabolized?

If you not mind, can you type the answer.I do not understand handwriting in sometime. Please

Thanks

1. Describe one reason why rRNA used to determine evolutionary relationships between organisms.

2. Describe the original procedure used by Woese and his students to determine the SSU rRNA sequences.

3. Describe the evidence that Woese and Fox found that supported the idea that there were three domains of life.

4. Describe the evidence that Woese and Fox found that supported the idea that the chloroplast evolved from cyanobacteria.

How does the structure of mRNA relate to its function?

Why is it necessary to find the new vector plasmid concentration after undergoing digestion and the clean up?

During the digest clean up what buffers are used and what is the purpose of each one?

compare and contrast pseudopod, flagella and cillia ?

You are studying a microbial species that divides by binary fission. Which of these do you expect to happen in the culture as is grows in a laboratory setting? Check all that apply.

-you expect the size of each cell to greatly increase over time, rather than the number of cells increasing much

-you expect the total biomass (weight) of cells to increase over time

-you expect the biomass to stay the same

-you expect the number of cells in the culture to increase, rather than each cell increasing much in size

1) What is endosymbiosis? Which organelles may have arisen this way? What evidence is there for this theory?

2) Which cells in our bodies have cilia and flagella? Can you find other examples? How do these structures differ in structure and movement? How are they similar?

3) How do flagella differ between prokaryotes and eukaryotes?

In garden peas, inflated pod shape (I) is dominant to pinched pod shape (i) and yellow pea colour (Y) is dominant to green (y) pea colour. Plants heterozygous for these characteristics were selfed (note that this is not a test cross), and the following numbers of phenotypes were seen in the progeny: 556 inflated, yellow; 193 inflated green; 184 pinched, yellow; 61 pinched green Test the data for the involvement of two independently assorting genes using the Chi-square test (don’t forget to state your Null Hypothesis).

Write a 700-800 word APA format essay explaining the human blood type inheritance.

What are the important aspects of the vitamin including the most common functions/roles in the body? What are 5 top food sources of vitamins?

1. Explains the symptoms/conditions a person might develop if he/she is deficient or excess in this vitamin or mineral. If there are conditions that may lead to or cause deficiencies, those should be discussed.
2. Describe what may inhibit and/or improve the bioavailability of this nutrient. This information is found for most vitamins and minerals in the text.   If there is nothing in your specific section, look at the beginning of the fat soluble and major mineral chapters for more general information. Those two chapters have overall information on bioavailability for all vitamins and minerals.

Mr Nel is a chronic cigarette smoker for the last 20 years. He smokes between 30-40 cigarettes every day. He is overweight and has a sedentary lifestyle working for a computer company. At home he is always tired and not in the mood to exercise. Sometimes he has difficulty to breathe.

Predict the effect of mr Nel’s lifestyle on his spirogram, therefore his lung volumes and write a CONCLUSION. Refer to his tidal volume, inspiratory reserve volume, forced expiratory volume in 1 second and his total lung capacity.