Question

what would the gel electrophoresis look like if the reverse primer wasn't added to the PCR mix? I know that you won't get as much product but I am having trouble visualizing what this would look like on a gel.

Answer 1

The goal of PCR is to make enough of the target DNA region that it can be analyzed or used in some other way. For instance, DNA amplified by PCR may be sent for sequencing, visualized
by gel electrophoresis, or cloned into a plasmid for further experiments.he results of a PCR reaction are usually visualized (made visible) using gel electrophoresis. Gel electrophoresis
is a technique in which fragments of DNA are pulled through a gel matrix by an electric current,and it separates DNA fragments according to size. A standard, or DNA ladder, is typically
included so that the size of the fragments in the PCR sample can be determined.Using PCR, a DNA sequence can be amplified millions or billions of times, producing enough DNA copies to be
analyzed using other techniques. For instance, the DNA may be visualized by gel electrophoresis, sent for sequencing, or digested with restriction enzymes and cloned into a plasmid.
The reverse primer attaches to the stop codon of the complementary strand of DNA (the sense strand).It would allow the codon to touch the complementary strand of dna if it wasnt added to pcr mix.