Questions
You’re provided a lab protocol to perform a very short digestion of eukaryotic chromatin with micrococcal...


You’re provided a lab protocol to perform a very short digestion of eukaryotic chromatin with micrococcal nuclease, which should result in DNA fragments of 200 base pairs. You setup the experiment but get called away suddenly and are gone for a length of time. When you return to complete the experiment and run the gel to visualize the samples, it shows 146 base pair fragments. Explain what occurred.

In: Biology

Many environmental toxicologists feel that animal testing to determine the safety of industrial and pharmaceutical chemicals...

Many environmental toxicologists feel that animal testing to determine the safety of industrial and pharmaceutical chemicals for human and environment health will diminish in future. What methods or new approaches will take the place of animal testing to forecast chemical safety?

In: Biology

Cell Biology Question: How does defective DNA repair have an effect on cancer cell proliferation? (explain...

Cell Biology Question:
How does defective DNA repair have an effect on cancer cell proliferation? (explain please)

In: Biology

Please give a detailed response and describe how T cells coordinate the specific humoral immune response....

Please give a detailed response and describe how T cells coordinate the specific humoral immune response. Then describe how T cells coordinate the specific cell mediated immune response. For both, be sure to discuss all receptors involved, interleukins, all cell interactions and cell types.

In: Biology

3) What are “age structured” population models and how do they inform toxicological impacts on populations?...

3) What are “age structured” population models and how do they inform toxicological impacts on populations? Why are such models better indicators of toxicant effects compared to the theoretical models of exponential and logistic growth?

In: Biology

Design an experiment that tests the effect of body temperature on the aerobic capacity in an...

Design an experiment that tests the effect of body temperature on the aerobic capacity in an animal of your choice. What variables would you measure, and how? Include detail about experimental design and instrumentation, to show your understanding of how data are acquired. Present hypothetical data (in graphical form), which support your predictions. Make sure to label your graphs accurately and precisely, and use reasonable values (and units) for parameters measured.

In: Biology

In the Community Tolerance Test, the tolerance of representative species in a community exposed to a...

In the Community Tolerance Test, the tolerance of representative species in a community exposed to a toxicant is compared to the tolerance of species in a reference, non-exposed community. Why or how is tolerance expected to differ in the two communities, and what is the value of this test to ecotoxicology?

In: Biology

Air-breathing insects and vertebrates rely on aerobic metabolism for sustained production of ATP, but they effect...

Air-breathing insects and vertebrates rely on aerobic metabolism for sustained production of ATP, but they effect oxygen supply by completely different means. Using two species of your choice (one for each taxon), compare and contrast the way these two groups deliver O2 from the environment to their tissues. Consider the scaling laws for the gas transport cascade and explain why there are no insects larger than 100g, but there are plenty vertebrates larger than that.

In: Biology

Regarding the electron transport chain, are all of the complexes within the chain integral? Specifically, is...

Regarding the electron transport chain, are all of the complexes within the chain integral? Specifically, is complex 2 integral or peripheral? Some images display it as integral, meaning that it does enter the membrane but doesn't span the entirety of it like a transmembrane protein. These different images confuse me, so I'm hoping to get some clarification.

In: Biology

Can someone please compare and contrast sRNA regulators that act in trans and riboswitches?

Can someone please compare and contrast sRNA regulators that act in trans and riboswitches?

In: Biology

Maintenance of a relatively high and stable body temperature is considered a hallmark of endothermy. How...

Maintenance of a relatively high and stable body temperature is considered a hallmark of endothermy. How is it achieved and supported? Endothermy comes with considerable benefits, but also at a steep cost. What are the advantages and disadvantages of being endothermic? Explain in detail. Provide a hypothetical scenario (including specific physiologic modifications) how endothermy might have evolved in a vertebrate taxon from an ectothermic ancestor

In: Biology

Why is actively translated mRNA more stable in the cytoplasm, whereas non actively translated mRNA is...

Why is actively translated mRNA more stable in the cytoplasm, whereas non actively translated mRNA is rapidly degraded?

In: Biology

A 28-year-old woman gave birth to twins in 2014. Her set of twins shared a placenta,...

A 28-year-old woman gave birth to twins in 2014. Her set of twins shared a placenta, appearing to be identical twins.
But the 14-week ultrasound showed they were different genders -- making it impossible for them to be monozygotic twins.
These twins were formed when a single egg was fertilized by two sperm.
1. How many types of twins do exist?
2. What are "semi-identical" twins, and why are they so rare?
3. If this happen, do these embryos have fair chances of survival?
4. Which percentage of their genetic DNA do they share?

In: Biology

Maintenance of a relatively high and stable body temperature is considered a hallmark of endothermy. How...

Maintenance of a relatively high and stable body temperature is considered a hallmark of endothermy. How is it achieved and supported? Endothermy comes with considerable benefits, but also at a steep cost. What are the advantages and disadvantages of being endothermic? Explain in detail. Provide a hypothetical scenario (including specific physiologic modifications) how endothermy might have evolved in a vertebrate taxon from an ectothermic ancestor

In: Biology

Imagine that you are studying the metabolism of a baby who is feeding on breast milk....

Imagine that you are studying the metabolism of a baby who is feeding on breast milk. You are going to compare the catabolism of two molecules of galactose from the lactose in milk with the catabolism of two molecules of leucine from the casein of milk.

1. Detail the catabolic path * for the molecules until their final oxidation in CO2 + urea.

2. It includes all the reactions that are carried out and the enzymes that they catalyze.

3. Include the molecular structure and the name of all the intermediaries.

4. All of them must be intertwined. Use different colors for reactions that are (a) only from galactose catabolism (b) only from leucine catabolism and (3) common to both.

5. Finally, detail the net ATP gain from the complete oxidation of each of the molecules. (Include payment for making the urea molecule.)

In: Biology