Design a microscopy experiment (widefield) testing the effect of Vertiporfin on cell death (step by step). What do we look for? which colour are we running, how long are we running it for?

Note: don't worry about the microscope, just design an experiment for staining of cells so it can be looked under widefield microscope

19. Pick the correct answer.

In Drosophila, red eyes (E) are dominant over white (e) and normal wings (W) are dominant over shriveled wings (w). Use the addition and multiplication rules to calculate the phenotypic ratios of the cross of Eeww and EeWw.

1. Cross of Ee x Ee:

2. Cross of ww x Ww:

3. Red eyes and normal wings:

4. Red eyes and shriveled wings:

5. White eyes and normal wings:

6. White eyes and shriveled wings:

Louise is a 20-year-old woman who has been admitted to an acute mental health inpatient ward following an episode of self harming behavior.this is her first contact with mental health services. Louise presents with lacerations to both arms; some are superficial scratches, others, while of varying severity and depth, are not life-threatening. There are also visible scars on her arms from apparently previous incidents of self-harming behaviors.

Louise has been admitted via the local accident and emergency department following an initial CRISIS assessment by the hospital Mental Health Liaison Team. Louise had consumed half a bottle of vodka and also admitted to smoking cannabis prior to the incident. She denies having taken an overdose of any type and this is corroborated by normal blood test results. she was originally accompanied there by her mother who did not remain with her.

Louise does not appear remorseful for what she has done. She says she is depressed and angry with her parents who 'think she is worthless and do not care about me because  i haven't gone to university like my brothers'. She maintains that she doesn't need any help with her problems, claiming that they are down to 'my immaturity, as my parents keep telling me' and that I'll grow out of it with time.

Pathology

Define the patient's disease process

Describe the patient's disease process

Clearly explain etiology of the disease process

Clearly explain the cause of the disease process

Thoroughly explain signs and symptoms of pathology

explain what is meant by the following statement:

“All viruses mutate.  However, a mutated virus is not necessarily more dangerous.”

Include answers to the following questions:

What is a mutation?

Are most mutations helpful or harmful to a virus?  Why?

What would make a virus “more dangerous” to humans?

How is Bactoprenol involved in peptidoglycan synthesis?

Please provide an in depth answer in its simplest form for easy understanding. Thank you

Part A

Please pick one behavioral, biomedical, or psychosocial treatment method. In thinking about this method, what are 2 benefits and drawbacks? Please be sure to be detailed in your discussion. (Using: Treatment method for Alcohol Abuse).

Part B

Find a scholarly research article which discusses the effectiveness of the treatment method you chose to discuss in Part A of this forum. What was the population under study? were the methods used, results, and practical implications of this study? Do you think the results of this study would be effective for all population? Why or why not? Would another treatment have worked better for the population under study? Why or why not? Please do not discuss vaccinations for opioid addiction, or the nicotine patch.

Write down the details of your strategy for breeding chihuahuas starting from a small ANSWER group of grey wolves. Don’t worry about the time that your strategy may require, but simply describe the step-by-step approach you would follow and explain why you think it would work.

Scientists decided to study, in vitro, the activity of the eukaryotic DNA polymerase Pol e.
1) In a first experiment, they apply a solution of Pol e on top of a chromatography column containing heparin. They collect the solution that elutes from the bottom of the column (flow-through). Their analysis reveals that the flow-through does not contain any protein.
What is the property of heparin demonstrated by this experience?

2) They designed a second experiment including 4 steps.
Step 1: A long single stranded DNA (M13) was incubated with a radiolabeled primer (P) with a sequence complementary to a short region of M13.
Step 2: The DNA/P complex was then incubated with the DNA polymerase, the 4 types of deoxyribonucleoside 3-phosphate (dNTPs), with or without heparin.
Step 3: After incubation for different length of times (0, 1, 2, and 4 minutes) the DNA polymerase is inactivated, the DNA is extracted and separated by agarose gel electrophoresis.
Step 4: The radioactive DNA was visualized by autoradiography.

Scientists compared the activity of two DNA polymerase: and Intact polymerase (Pol e WT) and, a mutant polymerase lacking a portion of the protein (Pol e D). Not that the deletion does not affect the catalytic activity of DNA Pol e.
The results of the experiments are listed in the figure below.

a) What does the 31 nucleotides (nt) fragment represent? Be specific
b) Why do you think the 31 nt fragment was not detected when Pol e WT was incubated with heparin?

c) By comparing the data for Pol e WT and Pol e D and based on what you know about this polymerase, could you identify the domain of Pol e missing in Pol e D Briefly explain?

1. What is one major issue in the public health arena that you think merits more attention than it is currently getting from the healthcare establishment, the media or the community at large? Explain why you offer that issue and how you think it might be best addressed in the future.

Suggest a risk assessment model for enzymes to be used for food purposes.

David, an undergraduate intern in our lab, forget to block the blot with BSA and continue to the antibody hybridization steps and finally developed his blot. Could you please describe his blot picture without even looking!