Question 7

A strain of E. coli is grown under aerobic conditions. However, this strain has an inactivating mutation in the enzyme that converts Pyruvate to Acetyl-CoA. What is the consequence of this mutation?

1. The organism cannot fully oxidize glucose, but can metabolize fats and some amino acids

2. The organism can metabolize as normal

3. The organism cannot carry out glycolysis

Question 4

Which of the following organisms are directly beneficial to vascular plants?

I need a detailed essay about 600 words to understand for this topic : bacterial impacts on the environment and human life.

I need a detailed essay about 600 words to understand for this topic : gene expression regulation of both prokaryotes and eukaryotes and compare them.

Q1 A research laboratory identified a gene X of medicinal value in a plant species. You are given a small fragment of DNA containing gene X and the cloning vector pZoom. Maps of the 4 kb cloning vector pZoom and the 10 kb plant DNA fragment are shown in Figure 1 and 2, respectively. The PCR primer pairs (shown in Figure 1) F1/R1, F2/R2 and F3/R3 amplify fragments of 0.1, 0.6 and 0.8 kb, respectively. The antibiotic resistance gene A and gene B code for chloramphenicol and streptomycin resistance, respectively. The three restriction enzymes that cut this vector are BamHI, XbaI and HindIII represented on the map as enzymes I, II and III, respectively. You are given purified DNA of both the vector and the gene X containing DNA fragment at 0.2 µg/ul. Create a recombinant plasmid containing the complete gene X in the provided cloning vector pZoom. a. Design restriction digestion reactions using appropriate enzymes in such a way that you get a final concentration of 50 ng/µl for the digested vector and for the plant DNA (insert DNA) in the reaction. Your digestion should include all components in a 20 µL reaction. All enzymes are supplied with a concentration of 10 units/µL; you may use 1 µL of the enzyme in each reaction. Buffers for each enzyme are available as 10 times concentrated (10X) stocks. b. Generate three ligation reactions with 1:1, 2:1 and 3:1 molar ratios of the insert and the vector DNA. Your ligation should include all components in a 30 µL reaction. Keep the vector amount fixed at 100 ng per ligation reaction. You are provided with 10 x ligase buffer and DNA ligase (0.5 U/µL) to set up your ligations. c. Develop a strategy to select transformants and a quick method to screen recombinants; show your screening method using a figure. Predict the expected results with an explanation. d. Devise a restriction analysis method to confirm the desired recombinant; use a single most appropriate enzyme. Calculate the expected sizes of the restriction fragments from each, the vector and the desired recombinant. e. Devise a PCR strategy to confirm the desired recombinant. Calculate the expected sizes of the PCR fragments from the desired recombinant in a multiplex PCR including all three primer pairs. How will these sizes differ from the same multiplex PCR if the template is vector DNA instead of the recombinant plasmid?

Your task is to solve the following problems. For each, you must provide a written response and a supporting Punnett square. Your Punnett square must be drawn by hand.

The ability to roll the tongue sideways (A) is dominant over the inability to roll one's tongue (a). In humans, non-attached earlobes (B) are dominant over attached earlobes (b). Two parents with attached earlobes can roll their tongues. They are both heterozygous for the tongue rolling trait.

1. What is the genotype of the mother?
2. What is the genotype of the father?
3. Whare are the possible gametes from the mother?
4. What are the possible gametes from the father?
5. Draw the Punnett square, and combine the gametes to produce offspring.
6. What is the phenotypic ratio of these offspring?

Explain how the cytoskeleton participates in the functional organization of tissues, and discuss the role of adhesive molecules.

3. Describe the difference between a homologous chromosome and a sister chromatid

How does an animal interpret the location of a sound?  

part of the brain is responsible for integrating this?

(a) A full explanation of the role of the endocrine and nervous systems in maintaining homeostasis.

(b) A detail explanation for both recessive and dominant genetic disease inheritance. Please answer this two question

1. explain the difference between an ionic, covalent, and hydrogen bond.

2. draw and describe the electronic configuration pf NaCI before and after the transfer of electrons.

3. what are the four major macromolecules and what are the corresponding building blocks from which each of these macromolecules are made?

4. draw and describe an example of a reaction that shows how a dipeptide is formed and broken down.

5. explain how each of the macromolecules contributes to the development and maintenance of hair.

6. describe each level of protein structure: 1. Primary, 2. secondary, 3. tertiary, 4. quaternary. what type of chemical bonds stabilize these different structures and how?

7. briefly compare and contrast oxidizing and reducing reagents and the role that they play in building chemical bonds.

8. what happens to the protein structure (keratin) of curly hair after a chemical relaxer and heat are applied and what happens to straight hair after a chemical perm or relaxer is applied?

Which of the following statements is a false statement about the secondary immune response?

a.) the secondary immune response produces less antibodies compared to the primary immune response

b.) the secondary immune response is a targeted response to a specific pathogen

c.) the secondary immune response is part of the adaptive immune system

d.) the secondary immune response is faster acting in the presence of a pathogen compared to the primary immune response

e.) all of the answer choices are correct statements about the secondary immune response, none are false

Some sports such as figure skating, diving, dance, horse racing and boxing are more likely to place female athletes at risk for eating disturbance or disorder development. Discuss why you think these types of sports are more likely to place athletes at risk?

Complete the following table by writing the name of the cell structure or organelle in the right hand column that matches the structure/function in the left hand column. Structure/Function Cell structure

1. May store water, needed chemicals and wastes in plant cells

2. The “sugar factory” in plant cells that contains chlorophyll

3. Small structure that makes protein

4. Long whip-like structures used for movement

5. The region of the cell between the plasma membrane and the nucleus

6. Cell control center

7. Sac of digestive enzymes

8. Boundary between the cell and its surroundings

9. Small hair-like structures used for movement

10.Produces a usable form of energy (ATP) for the cell

11.“Ships” products to plasma membrane for export outside the cell

12.Consists of hollow tubes which provide support for the cell

13.Lipids manufactured here

14.Numerous ribosomes give it its name

15.Breaks down drugs and toxin in liver