1. There are three sets of assay data obtained from a set of standard solution in an ELISA experiment (triplicate assay). Use Excel to draw a calibration curve. Then do the following:

(a) Calculate the Lower Limit of Detection (LLOD)

(b) Calculate the Limit of Quantitation (LOQ)

(c) Calculate the Sensitivity of the assay

(d) If an unknown sample gives an assay reading of 1.35. Use the calibration curve to determine the concentration of this sample.

I am doing a project on cancerous cell types and I've already identified 20 kinds, the tissues they effect, and the exact disease they cause. There are multiple other steps to the project that I am struggling with. This involves finding biochemicals (such as lipids, proteins, etc) associated with specific cancer cell types, the metabolic pathways they effect, membrane proteins (such as growth factors) and membrane protein transporters associated with the cancer cell type.

Could someone give me an idea on what databases or websites to search for for this type of information? I don't want the work done for me, just some hints on how I can find this information. I will list the exact instructions our professor gave us below.

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Use your Human Cancerous Cells examples from Module I and find if they have some very characteristic biochemical(s) that define the cells.

Describe chemicals that are unique for these cells
Give at least 5 examples.

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Use your examples of human cancerous cells and find affected metabolic pathways that identify each of them.

Affected metabolic pathways
Find at least 5 examples from your cancerous cells that have affected metabolic pathways. These examples should have different affected pathways

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Use your examples of human cancerous cells and identify possible membrane components that are affected and unique for these type of cells.

Identify affected membrane functions
Give at least 5 examples from your cancerous cells showing affected membrane proteins. These examples should have different affected proteins.

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Use your examples of human cancerous cells and identify possible membrane protein transporters that are affected and unique for these type of cells.

Affected transporting proteins
Identify affected membrane protein transports
Give at least 5 examples from your cancerous cells showing affected membrane proteins that are involved in transportation. These examples should have different affected proteins.

The lab TA was recently putting away prepared tissue slides after lab last week, when she accidentally dropped the slide holder spilling all the slides. As she was picking them all up, she notices that the labels had come off of two of the slides. Based on the remaining slides, she figured out that one slide is from the intestine and the other slide is from the esophagus. Based on their location in the body, how could you figure out which slide is which? For each location provide the following information:

• The type of epithelium present.
• The function of the above identified epithelium in that location.

Conclusion about Monosaccharides, Disaccharides and Polysaccharides

Briefly discuss the role of the immune system in pathogenesis.

Explain various ways in which bacteria can attach to host cell surfaces.

Applying the Scientific Method to an Experimental Study---

Your friend claims that sunlight affects the temperature of water

^^ I need to develop an experiment to test this claim

What is the Null and alternative hypothesis ???

1. Identify the Independent variable for your experiment.

2. Identify the Dependent variable for your experiment.

3. List at least 3 variables that you would hold constant in your experiment.

4. Now design the experiment: Identify and use at least five of the following lab equipment pieces to design your experiment. Provide a brief description of the experiment. Include a description of the Control Treatment and the Experimental Treatment

What is DNA transformation, and how are competent cells involved in the process?

how dose NAOh affect planarian

Reverse electron transport makes unique ecological niches available to micro-organisms. Explain this statement.

A female patient has been suffering from continuous diarrhea for a week. The stool had a metal like smell. The patient is a nurse at an Assisted living home. She felt sick a week ago and her symptoms resembled that of one of her own patients. Her symptoms have been worsening to the point she is very sick and had to take time off. 1. What disease does she have? Name the causative agent. 2. How did she become infected with this microbe? Explain the pathogenesis of this disease. 3. What is the virulence factor and how would you treat this disease? 4. What would you advise the patient to do in the future to avoid such illness in particular in her line of work.

Speculate on what seem to be the major selective pressures acting upon the worldwide population of human beings at this time. Would the selective pressures be different from human populations that had no technology beyond the ability to make fire?

Describe the human and plant immune systems.

List enzymes that are needed to store blood glucose as glycogen following a meal