What is DNA transformation, and how are competent cells involved in the process?

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gladiator answered 1 year ago

PREPARATION OF COMPETENT CELLS and TRANSFORMATION: 2a. We will be preparing competent MM294 (E. coli) cells...

PREPARATION OF COMPETENT CELLS and TRANSFORMATION: 2a. We will be preparing competent MM294 (E. coli) cells using a chemical treatment of CaCl2. What are MM294 cells, why do we use them? Define “competent” cells and describe why/how CaCl2 makes cells “competent”. Why is “mid-log” phase important? Support your drawing with a cartoon of what cells “look like” before and after treatment. 2b. What does “heat-shock” do (in the transformation procedure)? 2c. What is LB? What does “LB” stand for and...

describe the process of gastric digestion. What cells are involved and what do they secrete? How...

describe the process of gastric digestion. What cells are involved and what do they secrete? How do they produce those secretions? How is gastric secretion and motility regulated by the GI tract, nervous system and endocrine system?

why is it important that the DNA preparation be sterile /free of cells for Transformation experiment?

Describe the experimental procedure used to accomplish DNA transformation into bacterial cells.

QUESTION 8 1. Are all bacteria competent for transformation? a. Yes they are all competent b....

QUESTION 8 1. Are all bacteria competent for transformation? a. Yes they are all competent b. No, some bacteria in nature can do it and in the lab scientists can force bacteria to be competent. o c. No and nothing can be done about it d. no only yeast are competent 10 points QUESTION 9 1. Which enzyme "glues" DNA fragments together? a. DNA Ligase b. DNA Recombinase c. all these enzymes d. DNA Reductase 10 points QUESTION 10 1....

Explain how DNA is replicated.Be sure to include the enzymes involved in the process.

Efficient oncogenic transformation of rodent cells: a. blocks DNA replication b. requires a mutation in at...

Efficient oncogenic transformation of rodent cells: a. blocks DNA replication b. requires a mutation in at least 3 or more proto-oncogenes c. requires a mutation in one protooncogene d. inhibits cell cycle e. lengthens G2 phase of the cells

what is transformation and selection? what is the descriptive process of selection and transformation?

What chemical you use to induce cell competent for transformation? What are three important features a...

What chemical you use to induce cell competent for transformation? What are three important features a vector should have? Why it is important to have the recovery process during transformation? What is the next step after recovery process?

9.) During the process of DNA replication, both DNA polymerase and RNA polymerase are involved in...

9.) During the process of DNA replication, both DNA polymerase and RNA polymerase are involved in eventually creating the new daughter strand of DNA. T or F 10.) Replication begins at the origin of replication or ORI and transcription begins at the first codon for methionine. T or F 11.) The process of mapping, sequencing and analysis of proteins is called genomics. T or F 12.) The heavy chain constant domains of a given immunoglobulin molecule define its class. T...

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