concept of horizontal gene transfer in bacteria and how it can occur through a couple of different routes. Name two routes of transfer and why this occurring is particularly concerning in clinical settings

Biological Role of Nardonella Endosymbiont in Its Weevil Host.

(Kuriwada et al 2010). Find online to answer.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0013101

(in your own words):

1. the research question / hypothesis of the paper.

2. Explain the significance of the data shown in Table 1, with regard to the methodology behind the experiments

3. Summarize the results and significance of Figure 3.

4. Did the authors actually demonstrate what is suggested in the title of the paper?

5. With regard to the weevils, is the relationship obligate or facultative, is the transmission of bacteria between generations horizontal or vertical, and what is the level of specificity between host and bacteria?

6. Describe a future experiment that should be conducted in order to follow up on outstanding questions that pertain to the subject of the

6. BSA has the following amino acid profile compared with the average of known vertebrate proteins:

Based upon this data, is BSA a good standard to use in the Bradford assay? Explain your answer.

The Na –glucose symport system of intestinal epithelial cells couples the \"downhill\" transport of two Na ions into the cell to the \"uphill\" transport of glucose, pumping glucose into the cell against its concentration gradient. If the Na concentration outside the cell ([Na ]out) is 153 mM and that inside the cell ([Na ]in) is 19.0 mM, and the cell potential is -49.0 mV (inside negative), calculate the maximum ratio of [glucose]in to [glucose]out that could theoretically be produced if the energy coupling were 100% efficient. Assume the temperature is 37 °C.

Suppose gene A is on the X chromosome, and genes B, C and D are on three different autosomes. Thus, A- signifies the dominant phenotype in the male or female. An equal situation holds for B-, C- and D-. The cross AA BB CC DD (female) x aY bb cc dd (male) is made.

A) probability of obtaining A- individual in F1

B) probability of obtaining an a male in the F1 progeny

C) Probability of A- B- C- D- female in F1

D) How many different F1 genotypes are there?

E) Probability of F2 individuals will be heterozygous for the four gens?

F) Determine a probability of each of the following types in the F2     individuals  

(1) A- bb CC dd (female);  

(2) aY BB Cc Dd (male);  

(3) AY bb CC dd (male);  

(4) aa bb Cc Dd (female)

Skeletal system 1.list the major organs in this body system. 2. Describe ( in detail) the normal function of this body system. 3. Identify and explain about 3 common diseases that affect this body system. Describe diagnostic testing used to detect the disease and treatment modalities for each. 4. List 2 changes that are relevant to the body structure and function of this body system in children. 5. List 2 changes that are relevant to the body structure and function of this body system in the elderly. 6. What is one weird,gross,strange,or interesting thing about this system?

1.)A.)The lac operon is inducible by the presence of lactose. Loss-of-function mutations in the lacI gene, which encodes the lac repressor (pick all that apply):

A.)slow down the isomerization of lactose into allolactose
B.)decrease the amount of permease activity in the cell in the presence of lactose.
C.)prevent loss-of-function lacZ mutations from making the lac operon uninducible
D.)are recessive to wild type

B.)Mutations that eliminate the lacO operator site (pick all that apply):

A.)slow down the isomerization of lactose into allolactose
B.)decrease the amount of beta-galactosidase in the cell in the presence of lactose
C.)prevent lacS superrepressor mutations from making the lac operon uninducible
D.)are recessive to wild type

C.)Loss-of-function mutations in the lacZ gene, which encodes beta-galactosidase (pick all that apply):

A.)slow down the isomerization of lactose into allolactose
B.)decrease the amount of permease activity in the cell in the presence of lactose
C.)prevent lac repressor from binding the lacO operator site
D.)prevent IPTG from inducing the lac operon

D.)You are studying the Tory operon, which encodes the TorA and TorB genes, and is normally inducible by Torose. You find a mutation, m1, that causes the Tory operon to be uninducible. You examine the merodiploid (m1 TorA+ TorB-)/(+ ToryA- TorB+) and find that Tor A is uninducible and TorB is inducible. You conclude that:

A.)m1 acts in cis
B.)m1 acts in trans

Enzymes (or their production) are often regulated in a negative or positive way, depending on whether they are related to anabolic (building) or catabolic (breaking down) reactions. Please describe, in your own words, why excess of products might inhibit enzymatic activity for anabolic reactions but why excess of reactants might stimulate enzymatic activity for catabolic reactions. This question is pretty complex, so take your time to develop your thoughts prior to typing your answer. The best answers should incorporate aspects of bioenergetics, genetic expression, and cell biology.