1. Do some research and explain how an antibody can be produced that reacts with another antibody

Erythromycin is an antibiotic used to treat bacterial infections, by binding in front of the A site on the 50S subunit of a ribosome.

Why is Erythromycin effective against bacterial infections while causing minimal side effects on human cells?

Group of answer choices

1Bacterial ribosomes are 80S; cytoplasmic ribosomes in eukaryotes are 70S (so the drug will only target the bacterial ribosome)

2.Bacterial ribosomes are 70S; cytoplasmic ribosomes in eukaryotes are 80S (so the drug will only target the bacterial ribosome)

3.Erythromycin targets both bacterial and human cell structures

4.Human cells do not have cell walls

Which of the following processes is directly inhibited by this drug?

Group of answer choices

replication

translation

transcription

horizontal gene transfer

1. Which of the following conditions does not lead to a change in gene expression patterns in bacteria cells?

A.

Sporulation

B.

Heat shock

C.

Nutrient availability

D.

Nitrogen deprivation

E.

None of the choices are correct

2. Predict the outcome of the removal of the N form the DNA sequence of the (lamda) phage:

A.

Lysogenic cycle will be induced

B.

Entry into the bacterial host will be blocked

C.

Antitermination will be affected

D.

Transcription form the left promoter will be interrupted

E.

Antitermination will be affected and transcription form the left promoter will be interrupted

3. Which of the following is a possible mechanism for the action of NusA in termination?

A.

It binds to the RNA polymerase causing it to stall.

B.

It stimulates termination at intrinsic terminator by facilitating hairpin loop formation

C.

It binds to NusB to promote detachment of the RNA polymerase for the DNA template

D.

It interacts with S10 to promote dissociation of the RNA polymerase

E.

It binds to the RNA polymerase causing it to stall and it stimulates termination at intrinsic terminator by facilitating hairpin loop formation

Use the following information for all questions in this exercise.

Protein information:

TEVP: Extinction coefficient: 32,290 M^-1cm^-1, MW: 28.6 kD

GFP-POI: Extinction coefficient: 63,300 M^-1cm^-1, MW: 65.9 kD

GFP: Extinction coefficient: 30,430 M^-1cm^-1, MW: 32.1 kD

POI: Extinction coefficient: 32,850 M^-1cm^-1, MW: 33.8 kD

A protein sample of 10 mls, A₂₈₀ 0.655, containing a His-tagged GFP-linked protein with TEV site was treated with 50 ?ls of 1 mg/ml His-tagged TEV protease, then applied to a Ni-NTA column. We will assume that the TEV protease was complete with its reaction. The following represent the pooled fractions from each elution.

Initial flow through + 2 mls column wash: 12 mls, A₂₈₀ = 0.283

Remaining column wash and low Im buffer had no significant absorbance  

High Im buffer: 6 mls, A₂₈₀ = 0.534

Questions:

1) What is the amount of GFP-POI in nanomoles?

- I know we use Beer's Law; however, I do not know what absorbance value we should be using.

2) What is the amount of TEVP added in nanomoles?

3) What is the concentration of the POI (in mg/ml)

4) Based on this concentration, convert the POI to nanomoles and compare to the amount of GFP-POI. If they are the same, then we can assume the initial pool of GFP-POI was pure. If they are different, then there must have been an impurity present. Is your sample pure? Answer yes or no:

5) What is the absorbance due to TEVP in the pooled fractions that contain it?

*Note: More info on topic - High imidazole buffer should elute both GFP and TEVP.

Please help, and thank you!

Colorblindness is an X-linked recessive trait. Let us assume that a colorblind man has a colorblind sister and his mother has normal vision.

What is the genotype, as to color vision, of this man’s mother and of his father?

What kind of color vision will his sons and his daughters have, and in what ratios, if he marries a woman identical in genotype to his mother?

13. Epigenetics basically refers to changes in gene activity without a change in base content of DNA. Think of a multicellular eukaryotic organism such as a human. Please write down 2 different genomic events in humans that may be associated with epigenetic regulation.

What is MALDI-TOF? How does it work? What can it tell us?

41. True or False- No Protists cause disease in humans?

42. Phages infects...

A. Protists

B. plants

C bacteria

D animals

43. Photoautotrophs use

A. Light as an energy source and methane as a carbon source

B. Light as an energy source and CO2 as a carbon source

c. CO2 as both an energy source and a carbon source

D. N2 as an energy source and CO2 as a carbon source.

Choose the correct type of expression for each example.

Describe the two non-living components of bone that provide strength and flexibility

26. How is the action of ACh different in cardiac cells than it is in skeletal muscle cells?

Need well written paragraphs about them .

1. Reconstruct the imaginary timeline of 4.55 billion years of Earth’s geologic time in terms of six days

2. Clarify the mistaken belief of some people that “Transition fossils don’t exist.”

why inoculating an EMB plate and an MSA plate potentially useful?