What is the purpose of Mineral Oil in the API-20E test?

What could happen if a purity plate is not set up from the bacterial suspension in the API test?

What happens if the oxidase test is positive for an organism being identified using an API-20E strip?

Why does bacterial growth decrease as the solute concentration increases? Explain this phenomenon.

A bacteria culture is grown for many generations in a medium in which the only available nitrogen is the heavy isotope (¹⁵N). The culture is then switched to a medium containing only ¹⁴N for TWO generation of growth; it is then recultured to a ¹⁵N-containing medium for ONE final generation of growth. The DNA from these bacteria is isolated and centrifuged to equilibrium in a CsCl density gradient.

(a) Determine the proportion of ¹⁵N¹⁵N, ¹⁵N14N, and ¹⁴N¹⁴N DNA if the replication is following the semiconservative mode. Show your work to gain maximum mark.

(b) Determine the proportion of ¹⁵N¹⁵N, ¹⁵N¹⁴N, and ¹⁴N¹⁴N DNA if the replication is following the conservative mode. Show your work to gain maximum mark.

Note: You may hand-sketch the drawing in a piece of blank paper, scan or take a photo of your drawing, and attached it in your Word document.

For the following: 1) Identify the genotypes of the parents 2) Complete a Punnett Square & 3) Give the genotypic AND phenotypic results of the cross (percentage OR ratio)

Problem:

In humans, male pattern baldness is an X-linked trait.

Show the cross between a male that is not bald and a female that is a carrier.

(Please write or type response, do not use cursive writing due to visual impairment)

Thank you.

Although candida species are not virulent as some microbial pathogens, the fungus can still invade every human tissue. Propose a possible explanation for this observation 10 marks

what are the steps involved in the metastatic cascade?

tell us about your 'unique' trait requires ENERGY. How do you think this is related to metabolic processes such as the Citric Acid cycle and Electron Transport chain?

I have an issue understanding maternally and paternally imprinted gene questions.

Here are some examples:

1. A phenotypically normal man named John is heterozygous for a deletion on chromosome 15 that contains the maternally imprinted gene SNPRN gene. Absence of SNPRN gene function results in Prader-Willi syndrome. The man marries a normal woman named Jane who does not carry the deletion. They have 2 children. Which of the following statements is FALSE?

A. John inherited the chromosome with the deletion from his mother

B. There is a 25% chance that both children will have Prader-Willi syndrome

C. Half of John's sperm contain a version of the SNPRN gene that would be expressed in his children

D. All of Jane's eggs contain a version of the SNRPN gene that would be expressed in her children

E. in Jane's somatic cells, there is one active copy of the SNRPN gene.

The answer is D, but how are choices B and E true? I'm in a genetics course at a university so being detailed would be helpful please.

2. The Igf2 gene in mice encodes a growth factor gene whose expression is necessary for normal size. The absence of the Igf2 gene products results in small mice. The Igf2 gene is a maternally imprinted gene, meaning it gets inactivated during oogenesis in females. A normal-sized female mouse heterozygous for a deletion of the Igf2 gene gives birth to a normal-sized male. This male is then crossed to a normal female (no deletion). Assuming that this male inherits the Igf2 deletion from his mother, what fraction of the progeny from this male will be small?

A. 1/4 B.0 C.1/2 D.1/3 E.1

The answer is C, but again I don't understand why.

Q. We have a recombinant inbred line population in the F10 generation (NOTE THAT THIS IS A RECOMBINANT INBRED POPULATION (RIL), NOT AN F2 POPULATION). In this population, it was observed that there were 62 RILs with purple flowers and 38 RILs with white flowers.

1. What is the expected number of individuals for each class if a single dominant gene conditions purple flower color? For example, the parents used to produce the RIL population could be WW x ww.

2. Calculate the Chi-square statistic for this expected ratio.

3. Does the observed number of purple vs white fit the expected ratio for a single dominant gene at the 0.05 level of probability?

Why would we choose a cDNA library over a genomic DNA library? In other words, what is the difference between the two?

Find and analyze an article (first published in the last 28 days) about some topic dealing with nutrition and/or exercise addressing a hypothesis. This can come from a newspaper, magazine or other periodical.Analyze the article and address the following:

1. What hypothesis was tested?

2. How was it tested?

3. Was the hypothesis supported or rejected?

4. Is the sponsor of the science mentioned? This could be a university where research was conducted, a company that supplied funding, etc. Do you think the sponsor may have influenced the science? By that I mean do you think the source of funding could lead to a conflict of interest?

5. What are some implications of this research?

6. What could you do if you wanted to find out more about the topic? Be more specific.

Make a comparative chart on the main methods of protein quantification.

Which of the following would normally alter the affinity of an enzyme for its substrate? Select all that apply.

answer choices

a.pH

b.Temperature

c.Ionic concentrations

d.Enzyme concentration

Outline the process of producing vaccines. Write a mini essay on vaccination, detailing the principle of vaccination preparation, development of new vaccines, the routes of administration and side effects. Briefly touch on vaccine controversy.

as an exercise physiologist what activities and educational modules they recommend for kids in terms of physical activity and physical fitness, eating for healthy living and community cohesiveness ?