Suppose that you isolated an enveloped helical RNA virus. Purified RNA from this virus is capable...

Suppose that you isolated an enveloped helical RNA virus. Purified RNA from this virus is capable of supporting translation in a rabbit reticulocyte cellfree translation system or in any other such system Further analysis indicates that this virus replication is inhibited if infected cells are incubated with protease inhibitor such as trypsin inhibitor.

Based on this information, discuss the mechanism by which this virus replicates its genome in infected susceptible, permissive cells.

Expert Solution

The above information provided is related to the virus replicates genome in permissible cell and infected susceptible.

gladiator answered 1 year ago

2. Suppose that you isolated an enveloped helical RNA virus. Purified RNA from this virus is...

2. Suppose that you isolated an enveloped helical RNA virus. Purified RNA from this virus is capable of supporting translation in a rabbit reticulocyte cell‑free translation system or in any other such system Further analysis indicates that this virus replication is inhibited if infected cells are incubated with protease inhibitor such as trypsin inhibitor. Based on this information, discuss the mechanism by which this virus replicates its genome in infected susceptible, permissive cells.

1. Suppose that you isolated an enveloped icosahedral RNA virus. Purified RNA from this virus is...

1. Suppose that you isolated an enveloped icosahedral RNA virus. Purified RNA from this virus is capable of supporting translation in a rabbit reticulocyte cell‑free translation system or in any other such system. Further analysis indicates that this virus replication is not inhibited by actinomycin D, a strong inhibitor of DNA-dependent RNA polymerase. Based on this information, discuss the mechanism by which this virus replicates its genome in infected susceptible, permissive cells. Please give more information that ties in replication...

Why do you suppose an animal virus grown in (and then isolated from) tissue culture might...

Why do you suppose an animal virus grown in (and then isolated from) tissue culture might be more susceptible to inactivation by antibody than a similar virus propagated (and then isolated from) in an animal? Assume you do the assay by isolating the viruses grown in these two different environments from antibody and then seek to compare in a controlled environment, which virus particle is inactivated more readily by the presence of appropriate antibody.

You have purified genomic RNA from the following viruses: Zika, and murine leukemia viruses. You performed...

You have purified genomic RNA from the following viruses: Zika, and murine leukemia viruses. You performed an experiment where susceptible, permissive cells were separately transfected with one pure molecule of viral RNA. Which RNA will lead to a productive viral infection? Discuss the reasons why one RNA succeeds and the other fails. Be clear and convincing

A bacterium isolated from sewage sludge was recently found to be capable of anoxygenic photosynthesis using...

A bacterium isolated from sewage sludge was recently found to be capable of anoxygenic photosynthesis using nitrite as the electron donor, converting it to nitrate. Compare this form of nitrification to that which is well characterized. By removing nitrite from the environment, what other processes within the nitrogen cycle might this bacterium be influencing?

Suppose a student carrying a flu virus returns to an isolated college campus of 1000 students.

Suppose a student carrying a flu virus returns to an isolated college campus of 1000 students. Determine a differential equation governing the number of students x(t) who have contracted the flu if the rate at which the disease spreads is proportional to the number of interactions between the number of students with the flu and the number of students who have not yet been exposed to it

What do you understand from helical CT? Discuss. What are the advantages of helical CT over...

What do you understand from helical CT? Discuss. What are the advantages of helical CT over the step and shoot sequential CT? What is the purpose of antiscatter grid in a CT instrument? Discuss its use in CT. What is filter back projection and how is it used in CT?

Describe how an RNA virus invades and destroys a cell. b. How does this differ from...

Describe how an RNA virus invades and destroys a cell. b. How does this differ from a DNA virus?

You have discovered a new virus that contains only RNA as its genetic material. Curious as...

You have discovered a new virus that contains only RNA as its genetic material. Curious as to how this virus works, you infect host cells with this virus and discover that after infection, the host cell makes lots of viral RNA molecules and various viral proteins. Upon further examination, you discover that the viral genome has been inserted into the host genome as DNA. These are the steps of the information flow for this virus. Each arrow represents a process...

You purified the plasmid from an overnight cell culture and dissolved the plasmid DNA in 100...

You purified the plasmid from an overnight cell culture and dissolved the plasmid DNA in 100 μL 0.1M Tris buffer (pH8). To quantify the plasmid, you took an absorbance measurement at 260 nm of a 1:50 dilution sample. The absorbance was 0.35. What is the concentration of the undiluted plasmid in micrograms per mL?

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