In: Biology
Make a comparative chart on the main methods of protein
quantification.
Method |
Reagents |
Equipment |
characteristics |
Bradford | |||
Biuret | |||
Lowry | |||
BCA (bicinconinic acid) |
Method |
Regents |
Equipments |
Characteristics |
Bradford |
Coomassie Brilliant Blur G-250 Preparation:
|
Light spectrophotometer (that can detect 595nm wavelength), Cuvettes |
It is a very fast, sensitive and accurate method for the estimation of protein |
Biuret |
Na-K-tartrate, CuSo4.5H2O, KI, Preparation: Take 9g (Na-K-tartrate) + 3g (CuSo4.5H2O) +5g (KI) in 500ml 0.2 M NaOH |
Light spectrophotometer (that can detect 550nm wavelength), Cuvettes |
It is time taking and low sensitive method |
Lowry |
Na-K-tartrate, CuSo4.5H2O, Folin reagent, NaOH, Na2Co3 Preparation:
|
Light spectrophotometer (that can detect 650nm wavelength), Cuvettes |
It is sensitive and time taking methods but used in which various interfering components along with proteins present in the analysing solution, This is a two-step process so needed more time |
BCA (Bicinconinic acid) |
BCA, Na-K-tartrate, CuSo4.5H2O, NaOH, Na2Co3 Preparation:
|
Light spectrophotometer (that can detect 562nm wavelength), Cuvettes |
As similar to the Lowry method. It is a single-step process so less time and technical error possible |