Why would we choose a cDNA library over a genomic DNA library? In other words, what...

Why would we choose a cDNA library over a genomic DNA library? In other words, what is the difference between the two?

Expert Solution

cDNA LIBRARY- cDNA stands for complementary DNA. To construct a cDNA library mRNA is obtained first, then with the help of reverse transcriptase, the DNA strand is produced which is then inserted into a bacterial plasmid. The plasmids are then inserted into a host cell ( bacterial cell) and cultured. Then isolation and purification of the DNA is done and the DNA is sequenced. Thus, the cDNA library represents the transcriptome (as the DNA is derived from mRNA). The size of the cDNA library is usually smaller than the genomic library.

Genomic DNA library- In this type, the library includes the total chromosome content of an organism. The coding and non-coding both parts are included in this type of library, thus the size of this library is much larger than the cDNA library. Because of the large size of the fragments, it is stored into plasmids, cosmids, yeast artificial chromosomes(YAC) and bacterial artificial chromosomes (BAC).

gladiator answered 1 year ago

Answer these questions regarding DNA libraries: In creating a genomic DNA library, what is the FIRST...

Answer these questions regarding DNA libraries: In creating a genomic DNA library, what is the FIRST step? a. Fragmenting the DNA into manageable pieces b. Screening clones c. Transforming recombinant DNA into bacteria d. Ligating DNA into plasmids Sometimes artificial chromosomes (AC) constructed from bacterial (BACs) or yeast (YACs) DNA are used to harbor genomic DNA for libraries (e.g., the human genome project). Why are BACs/YACs often used instead of traditional plasmid DNA for the construction of genomic libraries?...

Describe the basic process to clone a cDNA vs. genomic library using PCR technique

Discuss a) Genomic and complementary DNA (cDNA) libraries and b) The testing of pharmaceutical drugs.

summarize the process by which the genomic library of P.fluorescens DNA was created. In fact, I...

summarize the process by which the genomic library of P.fluorescens DNA was created. In fact, I don’t understand the question, please help me answer this question, if you would explain this question in a better way, that will be great!

If you had a genomic library for a particular organism, what would you need to do...

If you had a genomic library for a particular organism, what would you need to do in order to find a particular gene within that library? Explain in detail, showing that you understand the steps involved in this process.

The MIT Whitehead Institute must choose between two cDNA microarray machines to expand their high-throughput genomic...

The MIT Whitehead Institute must choose between two cDNA microarray machines to expand their high-throughput genomic laboratory. Both of these machines have the same function, and the firm will only choose one vendor from which to purchase their machines. The first machine, manufactured by Amersham Pharmacia (machine 1), will cost $350,000. The second machine, manufactured by PE Applied Biosystems (machine 2), will cost $300,000. The cost of capital for both of these investments is 9.5%. The life for both machines...

1. Which vector would be best suited for constructing a human genomic library with the largest...

1. Which vector would be best suited for constructing a human genomic library with the largest sized insect fragment? a) Plasmid b) T4 phage c) Lamda phage d) YAC e) BAC 2. Successfully cloning an insert into a vector results in the production of a recombinant molecule. Name three enzymes that are commonly required for high efficiency of cloning. 3. A trait that is expressed in only one sex even though the trait may not be sex linked is reffered...

What role does the Chelex play in the isolation of genomic DNA? A) binds DNA so...

What role does the Chelex play in the isolation of genomic DNA? A) binds DNA so that impurities can be washed away B) binds divalent metal cations that inhibit the PCR reaction C) prevents the single-stranded DNA from reannealing during PCR D) immobilizes DNA polymerase preventing its denaturation at high temperatures

You have constructed four different libraries: a genomic library made from DNA isolated from human brain...

You have constructed four different libraries: a genomic library made from DNA isolated from human brain tissue, a genomic library made from DNA isolated from human muscle tissue, a human brain cDNA library, and a human muscle cDNA library. You wish to clone the human gene encoding myostatin, which is expressed only in muscle cells. A. Assuming the human genome is 3×10^9 bp and that the average insert size in the genomic libraries is 100 kb, how frequently will a...

You have embark on a genomic library construction project. What is the strategy that you will...

You have embark on a genomic library construction project. What is the strategy that you will be taking to ensure successful cloning of genomics inserts into the pGLO vector - you are well acquainted with this plasmid molecule? You have been provided with a map of the pGLO plasmid. a) Where in the plasmid vector molecule would you insert the genomics fragments that you isolated and what restriction enzymes would you choose? b) how would you determine that indeed you...

Question