define the following terms: biotechnology, plasmid, selectalbe marker, multiple cloning site, restriction enzyme, restriction enzyme site,...

define the following terms: biotechnology, plasmid, selectalbe marker, multiple cloning site, restriction enzyme, restriction enzyme site, DNA transformation, annealing

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Expert Solution

1. Enhancement and betterment of biological process by techniques and tools known as biotechnology.

2. Plasmid is a extra chromosomal DNA that can replicate independently. Plasmid exits in bacterial cells and also occur in eukaryotes. Size 1-200kb.

3. A gene introduce into a cells specially into a bacterium or to cell in the culture. It confirm a trait suitable for artificial selection it encodes for a protein product these marker gene introduce into the plant genome to express a protein.

4. MCS also called polylinker a short segment of DNA which contain many restrictions site. Restrictions site within mcs are typically unique occuring only once within a given plasmid.

5. Enzyme produce by certain bacteria that cleaves DNA molecule into fragments at or near specific recognition site known as restriction enzyme.

6. R.E. are cleaves on a specific site are known as restriction enzyme sites.

7. DNA transformation is process in which direct uptake and incorporation of exogenous genetic material from its surrounding through cell membrane transfer into the host cell.

8. Annealing is process of repairing of complementry sequence of single stranded DNA or RNA to pair by hydrogen bond to form a double stranded polynucleotides.

gladiator answered 1 year ago

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