If a circular plasmid is cut one with a restriction enzyme what is the result? how...

If a circular plasmid is cut one with a restriction enzyme what is the result?
how do we determine how many times a restriction enzyme cut.

Expert Solution

If a circular plasmid is cut one with a restriction enzyme what is the result?

Cutting of a circular plasmid once with a restriction enzyme would linearize the plasmid, if the plasmid has only one site for the restriction enzyme. If the plasmid has more than one site for restriction enzymes, the linearized plasmid would be fragmented into different pieces. Number of fragments depends on number of restriction site.

How do we determine how many times a restriction enzyme cut?

Agarose electrophoresis of digestion productions, after digestion with restriction enzyme, would give clear idea about number of restriction sites.

For instance, if the plasmid has 2 restriction sites for EcoRI enzyme, it would give 2 fragments (bands on the gel). Cutting at first site would linearize the plasmid, while cutting at the second site would cleave the linearized plasmid into 2 fragments.

If the plasmid has 3 restriction sites for EcoRI enzyme, it would give 3 fragments (bands on the gel)

gladiator answered 1 year ago

8. A circular plasmid of 6200 base pairs (bp) with three restriction enzyme sites at 900,...

8. A circular plasmid of 6200 base pairs (bp) with three restriction enzyme sites at 900, 1300, and 4000 bp. You digest this plasmid, then run the digest on a gel. What are the expected DNA fragment sizes? 9. A linear plasmid of 6200 base pairs (bp) with three restriction enzyme sites at 900, 1300, and 4000 bp. You digest this plasmid, then run the digest on a gel. What are the expected DNA fragment sizes? 10. In a random...

You digest a plasmid with a restriction enzyme that recognizes a single site on the plasmid....

You digest a plasmid with a restriction enzyme that recognizes a single site on the plasmid. When you perform gel electrophoresis on the digestion product, you quickly realize that there are two bands; one at the expected size and one near the well. Which of the following best explains the outcome? a.DNA was trapped in the agarose gel b.Some plasmids were not digested c.The presence of the chromosomal DNA d.The some plasmids ligated together

define the following terms: biotechnology, plasmid, selectalbe marker, multiple cloning site, restriction enzyme, restriction enzyme site,...

define the following terms: biotechnology, plasmid, selectalbe marker, multiple cloning site, restriction enzyme, restriction enzyme site, DNA transformation, annealing

What will you mix together in your restriction enzyme digest to cut out the gfp gene?

Imagine you have a plasmid and you cut it with three different restriction enzymes (enzymes 1,...

Imagine you have a plasmid and you cut it with three different restriction enzymes (enzymes 1, 2, 3). You then run these fragments through a gel and get this separation. Label the positive and negative terminals on this gel with a (+) and (-) sign. If you were working with a single plasmid, how many cut sites did restriction enzyme 1 have? Explain? When creating a gel, we stain it with Ethidium Bromide or Gel Red. What is the purpose...

You would like to sequence the genome of this organism. The restriction enzyme cut site for BglII is AGATCT.

A) You would like to sequence the genome of this organism. The restriction enzyme cut site for BglII is AGATCT. You will use it to clone fragments of the genome in order to sequence it. How many times would you expect this enzyme to cut within the chromosome of Andrebacillus wieczorius ? (2) B) You decide to use a modified bacteriophage vector that can accommodate fragments digested with BglII. You use a partial digest on the chromosomal DNA. What...

During “cut and paste” cloning, the PCR-amplified gene is cut by restriction enzymes. How does the...

During “cut and paste” cloning, the PCR-amplified gene is cut by restriction enzymes. How does the synthesis of DNA oligonucleotide primers facilitate the ability to ‘cut’ the amplified gene?

The commercially available pUC57 plasmid is circular with 2710 bp. It has one EcoRI cleavage site...

The commercially available pUC57 plasmid is circular with 2710 bp. It has one EcoRI cleavage site at 396 and one HindIII cleavage site at 471. How many bands would you expect to see on the gel if both enzymes were used to digest the enzyme and the digestion was complete? Explain your answer. Using the same plasmid and restriction enzymes as in question 2, how many bands would you expect to see on the gel if the digestion were partial?...

What can be the result of a genetic (DNA or plasmid) mutation in a prokaryote? Select...

What can be the result of a genetic (DNA or plasmid) mutation in a prokaryote? Select one or more: a. Endospore formation b. Antibiotic resistance c. Meiosis beginning d. Exponential decay in growth e. All of the above Which statement does not describe the structure of a virus? Select one or more: a. Some have a core of a nucleic acid (DNA) surrounded by a protein b. Some have a core of a nucleic acid (RNA) surrounded by a protein...

1. A plasmid is a A. Small circular segment of the endoplasmic reticulum that binds the...

1. A plasmid is a A. Small circular segment of the endoplasmic reticulum that binds the lacI repressor gene product. B. Segment of the bacterial genome that has been digested with a restriction endonuclease. C. Small circular segment of DNA that lacks an origin of replication (Ori) D. Small, circular segment of DNA that frequently contains antibiotic resistance genes. E. Segment of the nucleosome that has been digested with a restriction endonuclease. 2. Evidence that the lacI repressor gene works...

Question