Question

Please Transcribe and translate the normal sequence and the mutated sequence. Identify what type of DNA mutation occurred. (insertion, deletion, or substitution), and Identify what type of protein change resulted. (frameshift, missense, nonsense, or silent)

1) sequence: 3’ TAC CGA GGA ATG TAC GAC TGA ACG CTA ATT 5’.

mutated sequence: 3’ AC CGA GGA ATG TAC GAC TGA ACG CTA ATT 5’.

2)  sequence: 3’ TAC UUU CCU AAU AGG GGG GAC GCU UGU AGC ACG UAA 5’
mutated Sequence: 3’ TAC UUU CCU AAU AGG GGG GAC GUC UGU AGC ACG UAA 5’

Thanks so much:)

Answer 1

1) sequence: 3’ TAC CGA GGA ATG TAC GAC TGA ACG CTA ATT 5’.

Trancript : mRNA : AUG GCU CCU UAC AUG CUG ACU UGC GAU UAA

Translate : Amino acid sequences: Methionine- Alanine- Proline- Tyrosine- Methionine- Leucine-Threonine-Cysteine-Aspartate-Stop codon/nonsense codon.

mutated sequence: 3’ AC CGA GGA ATG TAC GAC TGA ACG CTA ATT 5’.

( 3'ACC GAG GAA TGT ACG ACT GAA CGC TAA TT 5')

DELETIUON of  T at 3' end leads to fram shift mutation. entire codons changed.

Transcript of mutated sequence: mRNA: UGG CUC CUU ACA UGA CUU GCG AUU

Translate of mutated sequence: no possiblity for traslation bec AUG is missing in the sequece

2) Sequence: 3' TAC UUU CCU AAU AGG GGG GAC GCU UGU AGC ACG UAA 5'

The given sequence has many deaminated nucleotide . That is cytosine is coverted to U. the proof reading enzymes will change that U to T.( Assume T in the place of U)

Transcript: 3'AUG AAA GGA UUA UCC CCC CUG CGA ACA UCG UGC AUU 5'

Translate: Methionine- lysine- glycine- leucine- serine- proline-leucine- arginine- lysine-serinre-cysteine- isoleucine

mutated Sequence: 3’ TAC UUU CCU AAU AGG GGG GAC GUC UGU AGC ACG UAA

Transcript:   3'AUG AAA GGA UUA UCC CCC CUG CAG ACA UCG UGC AUU 5'

Translate: Methionine- lysine- glycine- leucine- serine- proline-leucine- glutamine- lysine-serinre-cysteine- isoleucine

Here the nucleotides are changed CU is converted to UC. There ids no much change in amino acid composition arginine is replaced with glutamine ( both are basic amino acids)