Question

Data Tables and Post-Lab Assessment

Lab Questions

1.     Transcribe the following DNA sequence:

Hb A:

5’ AGT AAC GGC AGA CTT CTC CTC AGG AGT CAG GTG CAC CAT 3’

3'UCA UUG CCG UCU GAA GAG GAG UCC UCA GUC CAC GUG GUA 5'

2.     Translate your new RNA sequence using the genetic code. Remember that when determining your amino acid sequence, the RNA sequence is read from 5’ to 3’.

val- val -his- val- ser -ser- glu- glu- ser-ser- pro- leu -ser

Note: the first amino acid will be Met.

3.     Transcribe the following DNA sequence.

Hb S:

5’ AGT AAC GGC AGA CTT CTC CAC AGG AGT CAG GTG CAC CAT 3’

3`UCA UUG CCG UCU GCC GAG GUG UCC UCA GUC CAC GUG GUA 5`

4.     Translate your Hb S transcript.

val- val- his- val- ser-ser-val-glu- ser- ser- pro- leu - ser

Note: the first amino acid will be Met.

Post-Lab Questions

1.     How long are each of your peptide chains (how many amino acids are present)?

The proteins are made up of multiple units of amino acid. When we talk about peptide

chains they are combination of multiple amino acids. Each of the peptide chain consists

of many amino acids and the number varies.

2.     What differences exist between your two transcripts? What about your translated product?

3.     Hb A represents a section of the 626 base normal hemoglobin beta mRNA sequence. Hb S is from the sickle cell hemoglobin. Is the mutation found in sickle cell hemoglobin a point mutation or a frameshift mutation?

4.     Does the sickle cell mutation result in a missense mutation, silent mutation or nonsense mutation? Why?

A missense mutation is the cause of the disease, sickle cell anemia In a missense mutation, a change in one base pair causes a single amino acid to be changed in the resulting protein. In sicle cell anaemia there is missence mutation in ?-globin gene where glutamic acid (E/Glu) being substituted by valine (V/Val) at position 6.

Answer 1