What are the functions of the following enzymes in E.
coli?
DNA polymerase I
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DNA polymerase II
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DNA polymerase III
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DNA polymerase IV
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DNA polymerase V
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Please indicate (if possible) which is
the evolutionary history of these polymerases? Which of them you
could be eliminate and preserve the function of the organism? (you
must eliminate 2) What will be the consequences? Which
environment might be suitable for the survival of this “mutant”
that you created?
What would happen if we use Polymerase I instead of Polymerase
III for DNA replication in a PCR? If we used an enzyme with low
fidelity, how would the DNA product compare to the original
template?
What is the difference between the enzymes DNA polymerase I and
ligase?
Select one:
a. DNA polymerase I binds to Okazaki fragments, removes the
primers, and replaces them with DNA nucleotides.
b. Ligase binds to Okazaki fragments, removes the primers, and
replaces them with DNA nucleotides.
c. DNA polymerase I attaches the fragments to make one single
continuous strand.
d. Ligase attaches the Okazaki fragments to make one single
continuous strand.
e. Both A and D are correct.
f. Both...
1. Why does E. coli need both DNA polymerase III and DNA
polymerase I?
a. The DNA replication is bidirectional; one polymerase is used
for each direction.
b. Each polymerase is specific for only one strand of DNA. DNA
polymerase III acts only on the leading strand, and DNA polymerase
I acts only on the lagging strand.
c. Only DNA polymerase I has proofreading ability.
d. DNA polymerase III lacks the 5' → 3' exonuclease activity
needed to remove RNA...
I am trying to purify DNA polymerase from cells DNA polymerase
is a large soluble posivitely charged protein for each purification
step below select which fractions you will keep in order to end up
with pure DNA polymersase :
1. Cell fractioniation by centrifiguation:
a) Keep Nuclear Pellet
b) Keep mitochondrial Pellet
c) Keep microsomal supernatant
d) Keep microsomal Pellet
2) Salt fractionation with low salt concentration (low
salt):
a) Keep precipitate
b) Keep supernatant
3) Gel Filtration
a) Keep...
Doctors may be able to inject a functioning copy of the gene
mutated in Sam's DNA in the cells of his eyes. If the procedure
were successful, would you expect Sam to regain his vision? Why or
why not?
Question:
DNA polymerase III is the main DNA-synthesizing enzyme in
bacteria. Describe how it carries out its role of elongating a
strand of DNA.
Answer:
DNA pol III determines which free nucleotide triphosphate is
complementary to the base being copied. DNA pol III catalyzes
phosphodiester bond formation between the alpha phosphate of the
incoming nucleotide triphosphate and the 3’ hydroxyl group of the
last nucleotide added to the strand.
Can someone please explain this step by step in a dumbed...