1) Why was the Micrococcus at the bottom of the broth tube?
2) What growth pattern(s) would be most beneficial for survival if bacteria were growing in a pond for many years? Explain your answer.
In: Biology
a) Neanderthals
b) chimpanzees
c) Australopithecus
d) homo habilis
a) the immune system
b) cortex development
c) placental development
d) body growth
e) speech
a) interbreed
b) produce fertile offspring
c) share similar genes
d) all of these
a) DNA stabilization
b) new species formation
c) becoming closer in morphology in the populations
d) changes in alleles between the populations
e) b and d
a) corpus striatum
b) corpus collosum
c) corpus spongiosum
d) corpus quadrigemina
e) none of these
a) tool use
b) meat diet
c) brain enlargement
d) controlled fire usage
e) migration out of Africa
a) Neanderthals
b) Australopithecines
c) cro magnons
d) homo erectus
e) members of congress
a) thalamus
b) gyri
c) cerebral aqueduct
d) subarachnoid space
e) corpora quadrigemina
a) A. africanus
b) A. afarensis
c) homo erectus
d) homo habilis
e) c and d
a) haploid gametes
b) diploid gametes
c) specific groups of alleles
d) examples of genetic drift
e) none of these
In: Biology
If the phosphoglycerate mutase reaction the side chain of which as in thE enzyme is transiently phosphorylated as part of the reaction .,
In: Biology
Compare and contrast lytic and lysogenic infections in bacteria and humans.
In: Biology
Compare and contrast transduction (general and specific), transformation, and transfection
In: Biology
In humans, the concentration of glucose in the blood is maintained at roughly 5 mM. Hexokinase (HK II) in muscle is a Michaelis-Menten enzyme and its KM for glucose is about 0.1 mM. The blood glucose and intracellular glucose concentrations rapidly equilibrate so that the cellular glucose concentration can be considered to be essentially the same as the blood glucose concentration. Also, prolonged high concentrations of glucose in the blood can be toxic.
a) What do the KM and the actual glucose concentration suggest about the activity of HK II under normal conditions? (In other words, how fast is the enzyme going compared to its Vmax?)
b) After a high carbohydrate meal, the blood glucose concentration can rise to concentrations much greater than 5 mM. What would be the effect of this concentration change on HK II activity in muscle tissue?
c) Given the information and your answer in part b, what would be the net effect on the person’s blood glucose concentration after a high carbohydrate meal?
In: Biology
Please answer all of the questions.
Spider-Man and Spider-Woman are planning to have children in the near future. Spider-Man is able to spin webs (S) and cling to walls (C), whereas Spider-Woman can spin webs but cannot cling to walls. If both of these traits are inherited in a dominant manner (i.e., the dominant trait will always mask the recessive trait), Spider-Man is heterozygous for each trait, and Spider-Woman is heterozygous for the web-spinning trait...
What would be the phenotypic ratio of the F1 generation? Please note that: "S" = ability to spin webs; "NS" = cannot spin webs; "C" = ability to cling to walls; and "NC" = cannot cling to walls.
a. |
2 S/C : 6 S/NC : 6 NS/C : 2 NS/NC |
|
b. |
4 S/C : 4 S/NC : 4 NS/C : 4 NS/NC |
|
c. |
8 S/C : 2 S/NC : 2 NS/C : 4 NS/NC |
|
d. |
6 S/C : 6 S/NC : 2 NS/C : 2 NS/NC |
|
e. |
None of the above |
In humans, the ability to roll one's tongue is a dominant trait (R). A woman who can roll her tongue is married to a man who cannot roll his tongue. The couple's daughter, Nia, can roll her tongue, but their son, Michael, cannot.
What is the genotype of the mother?
a. |
RR |
|
b. |
Rr |
|
c. |
rr |
|
d. |
Either A or B |
|
e. |
Either B or C |
In humans, the ability to roll one's tongue is a dominant trait (R). A woman who can roll her tongue is married to a man who cannot roll his tongue. The couple's daughter, Nia, can roll her tongue, but their son, Michael, cannot.
What is the genotype of the father?
a. |
RR |
|
b. |
Rr |
|
c. |
rr |
|
d. |
Either A or B |
|
e. |
Either B or C |
In humans, the ability to roll one's tongue is a dominant trait (R). A woman who can roll her tongue is married to a man who cannot roll his tongue. The couple's daughter, Nia, can roll her tongue, but their son, Michael, cannot.
What gametes does the mother produce?
a. |
R |
|
b. |
r |
|
c. |
R and r |
|
d. |
Rr |
|
e. |
RR |
In humans, the ability to roll one's tongue is a dominant trait (R). A woman who can roll her tongue is married to a man who cannot roll his tongue. The couple's daughter, Nia, can roll her tongue, but their son, Michael, cannot.
What gametes does the father produce?
a. |
R |
|
b. |
r |
|
c. |
R and r |
|
d. |
Rr |
|
e. |
RR |
In humans, the ability to roll one's tongue is a dominant trait (R). A woman who can roll her tongue is married to a man who cannot roll his tongue. The couple's daughter, Nia, can roll her tongue, but their son, Michael, cannot.
If Nia were to (one day) marry a man who was heterozygous for the tongue-rolling trait, what percentage of their children would be able to roll their tongues?
a. |
0% |
|
b. |
25% |
|
c. |
50% |
|
d. |
75% |
|
e. |
100% |
In humans, the ability to roll one's tongue is a dominant trait (R). A woman who can roll her tongue is married to a man who cannot roll his tongue. The couple's daughter, Nia, can roll her tongue, but their son, Michael, cannot.
Imagine, for a moment, that the mother and father separate, and the mother decides to remarry. If her second husband is homozygous dominant for the tongue-rolling gene, what is the likelihood that their children (if they choose to have any) will be able to roll their tongues?
a. |
0% |
|
b. |
25% |
|
c. |
50% |
|
d. |
75% |
|
e. |
100% |
In humans, the ability to roll one's tongue is a dominant trait (R). A woman who can roll her tongue is married to a man who cannot roll his tongue. The couple's daughter, Nia, can roll her tongue, but their son, Michael, cannot.
Imagine, for a moment, that the mother and father separate, and the mother decides to remarry. If her second husband is homozygous dominant for the tongue-rolling gene, what would be the genotypic ratioof their offspring (i.e., the F1 generation)?
a. |
1 RR : 2 Rr : 1 rr |
|
b. |
2 RR : 2 rr |
|
c. |
2 RR : 2 Rr |
|
d. |
2 RR : 1 Rr : 1 rr |
|
e. |
None of the above |
Characteristics (or traits) of an organism, such as those described in the examples above, are said to be heritable. In this context, the term "heritable" means...
a. |
Able to induce production of hair in various species |
|
b. |
Traits that are most frequently seen within a given population early in development |
|
c. |
The ability for traits to be passed down from parent to offspring |
|
d. |
Traits that are only present when both parents are homozygous dominant |
|
e. |
None of the above |
In: Biology
Please answer all the questions
Spider-Man and Spider-Woman are planning to have children in the near future. Spider-Man is able to spin webs (S) and cling to walls (C), whereas Spider-Woman can spin webs but cannot cling to walls. If both of these traits are inherited in a dominant manner (i.e., the dominant trait will always mask the recessive trait), Spider-Man is heterozygous for each trait, and Spider-Woman is heterozygous for the web-spinning trait...
What is Spider-Man's genotype?
a. |
SSCc |
|
b. |
SsCc |
|
c. |
SSCC |
|
d. |
ssCC |
|
e. |
Sscc |
Spider-Man and Spider-Woman are planning to have children in the near future. Spider-Man is able to spin webs (S) and cling to walls (C), whereas Spider-Woman can spin webs but cannot cling to walls. If both of these traits are inherited in a dominant manner (i.e., the dominant trait will always mask the recessive trait), Spider-Man is heterozygous for each trait, and Spider-Woman is heterozygous for the web-spinning trait...
What is Spider-Woman's genotype?
a. |
SSCc |
|
b. |
SsCc |
|
c. |
SSCC |
|
d. |
ssCC |
|
e. |
Sscc |
Spider-Man and Spider-Woman are planning to have children in the near future. Spider-Man is able to spin webs (S) and cling to walls (C), whereas Spider-Woman can spin webs but cannot cling to walls. If both of these traits are inherited in a dominant manner (i.e., the dominant trait will always mask the recessive trait), Spider-Man is heterozygous for each trait, and Spider-Woman is heterozygous for the web-spinning trait...
What gametes does Spider-Man produce?
a. |
SS and Cc |
|
b. |
SC and Sc |
|
c. |
Ss, sS, Cc, and cC |
|
d. |
SC, Sc, sC, and sc |
|
e. |
Sc and sc |
Spider-Man and Spider-Woman are planning to have children in the near future. Spider-Man is able to spin webs (S) and cling to walls (C), whereas Spider-Woman can spin webs but cannot cling to walls. If both of these traits are inherited in a dominant manner (i.e., the dominant trait will always mask the recessive trait), Spider-Man is heterozygous for each trait, and Spider-Woman is heterozygous for the web-spinning trait...
What gametes does Spider-Woman produce?
a. |
SC and Sc |
|
b. |
SC, Sc, sC, and sc |
|
c. |
SC |
|
d. |
Sc and sc |
|
e. |
None of the above |
Spider-Man and Spider-Woman are planning to have children in the near future. Spider-Man is able to spin webs (S) and cling to walls (C), whereas Spider-Woman can spin webs but cannot cling to walls. If both of these traits are inherited in a dominant manner (i.e., the dominant trait will always mask the recessive trait), Spider-Man is heterozygous for each trait, and Spider-Woman is heterozygous for the web-spinning trait...
What percentage of their offspring will not be able to spin webs but will be able to cling to walls?
a. |
0% |
|
b. |
12.5% |
|
c. |
25% |
|
d. |
37.5% |
|
e. |
75% |
Spider-Man and Spider-Woman are planning to have children in the near future. Spider-Man is able to spin webs (S) and cling to walls (C), whereas Spider-Woman can spin webs but cannot cling to walls. If both of these traits are inherited in a dominant manner (i.e., the dominant trait will always mask the recessive trait), Spider-Man is heterozygous for each trait, and Spider-Woman is heterozygous for the web-spinning trait...
What percentage of their offspring will be heterozygous for both traits?
a. |
0% |
|
b. |
12.5% |
|
c. |
25% |
|
d. |
37.5% |
|
e. |
50% |
In: Biology
1. Life on Earth was pretty simple for over 3 billion years.... then suddenly, around 530 million years ago, a huge diversity of animal forms suddenly (~10 million years time) appear in the fossil record. What might have caused animal forms to appear form?
2. 20% of all animal species are beetles! New species of animals are still being found, mostly invertebrates. How many species do scientists believe are out there?
In: Biology
1. Describe DNA synthesis and describe why Okazaki fragments are needed? include the following terms in any order (and please underline them in your answer): leading strand, lagging strand, replication fork, DNA polymerase, direction of DNA synthesis, origin of replication, primer, ligase.
In: Biology
1. Describe the exocrine function of the pancreas. How is it related to digestion of the energy-yielding nutrients?
2. If you want to maintain a feeling of fullness for as long as possible, which meal would be preferred: 800 calories of spaghetti with tomato sauce, bread, and tea or 800 calories of spaghetti with tomato and meat sauce, bread with butter, and water? Why?
In: Biology
Background Calculating Transformation Efficiency Restriction digest & ligation: You add restriction enzymes to 0.05ug of each plasmid in separate pAMP and pKAN tubes tubes and heat the restriction enzymes by placing them in a hot water bath for 20 minutes. Afterwards, you label 1 tube pAMP/pKAN and combine 4 ul of digested pAMP, 4 ul of digested pKAN, 1 ul of ligase, and 1 ul of ligase buffer and leave it to incubate overnight. You then label an empty tube -pKAN/-pAMP which will be used as your control (no plasmids). Bacterial Transformation: To the 2 microcentrifuge tubes labeled +pKAN/+pAMP and -pKAN/-pAMP you add 250 µl of transformation solution (0.05M CaCl₂). Using aseptic techniques you transfer bacteria colonies into the -pKAN/-pAMP and +pKAN/+pAMP tubes making sure to avoid cross contamination. You incubate the tubes for 10 minutes in ice, transfer them to a water bath for 50 seconds, and then back in ice for 2 minutes. You then pipette 250 uL broth into each tube and incubate them for 10 minutes to make the bacteria happy again. Afterwards, you pipette 100 ul of each transformation mixture and spread it onto the appropriately labeled agar plates. You parafilm and incubate the plates of E. coli overnight. The next day you got the following results.
The questions that I need help on: Calculate the transformation efficiency in CFU/ug of DNA from the +LB/AMP/KAN plate. The concentration of the pKAN/pAMP is 0.1 ug/ul. The amount of plasmid DNA spread on the plate was 1 ug.
DNA spread (ug)= Volume spread on plate (ul) X DNA in transformation (0.05 ug) Total volume of transformation (add up all the highlighted volumes)
|
In: Biology
In: Biology
In: Biology
Place the following scenario in order of how it would occur... 1 to 7
S.typhimurium cells are allowed to grow and proliferate through various virulence factors which further allows it to express more virulence factors.
S.typhimurium cells use oral fecal route from infected host to leave one host and enter another host via potential contaminated surfaces or common sources.
S.typhimurium cells spreads along the lower GI tract (smaller intestine and larger intestine), virulence factors like enterotoxins create ion imbalance
You and your friends are out for a late night meal and you eat some undercooked chicken. Your system is exposed to S.typhimurium
S.typhimurium extracellular factors like Type 1 Fimbriae bind onto epithelial cells of the intestines.
Ion imbalance cause influx of water into the lumen while endotoxins cause symptoms like fever.
Various antigens present on S.typhimurium evade host systems like phagocytosis or macrophage detection.
In: Biology