can you please explain modes of transmission of e.coli

and please please explain virulence factors of E.coli in simple details.

thank you so much

72 year old man, complaining of flu like symptoms, but without a fever.He also has a rash on the right side of his body. The rash consists of fluid filled blisters is very painful. Worst pain he has every had.Checking with our medical intern, he asks if during the patient history, if we asked our patient if he had the chicken pox as a child and if he has been vaccinated against shingles.Why would a childhood disease be important now?…. And what does a vaccine have to do with this?please don't answer this question unless you know the complete answers to the case study form below.This case study is for nursing class.

Below is the Case Study Form:

Patient History:

Patient #:_______________________

Age:___________________________

Sex:___________________________

Occupation:______________________

Family History/Preconditions:

Relevant Recent History:

Signs and Symptoms:

Signs:

Symptoms:

Microbiology:

Causative Organism(s):_________________

Modes of Transmission:_________________

____________________________________

Prevention:___________________________

Virulence Factor:

Mechanism of Virulence Factor:

Treatment:

Drug Name:__________________________

Class of Drug:________________________

____________________________________

Type of Admission:____________________

Potential Side Effects of Drug Treatment:

Mechanism of Treatment:

Lab Work:

Type of Sample Received:_______________

____________________________________

Test(s) To Determine Causative Organism:

Positive Results of Test(s):

How does antibiotic resistance happen (from molecular mechanisms to HGT to societal factors)?

Define integrated control in your own words. What element of integrated control is the most challenging? Why? What are the challenges facing the development of an effective malaria vaccine.

An E. coli mutant isolated is simultaneously unable to utilize a large number of sugars, e.g. lactose, arabinose, maltose, as carbon source.  However, genetic analysis shows that each of the operons responsible for metabolism of each sugar is free of mutation.  Name a specific gene the mutation of which may account for this outcome?   Explain.

make a tree for the Chordates – include changes here too

SDS-Page Questions : Please answer all questions & explain.

1.Can I use SDS- page to diagnosis the urea cycle disorder : Arginase deficiency?

2.Would the mutated arginase have a different MW compared to the normal arginase?

3.Could you recognize/detect the mutated arginase on SDS- Page?

Which of the following is true about interferon?

It is released by uninfected cells during a viral infection

It is produced by virally infected cells during a viral infection, whereupon it inhibits further viral replication within the cells where it is made.

It is produced by virally infected cells to activate helper T-cells.

It is released by virally infected cells to induce an antiviral state in uninfected cells. It is released by cells in lymph nodes to attract T cells to the lymph node.

Question 3a:

Translation is another name for protein synthesis. How does the ribosome know that it should terminate translation?

Describe briefly what happens to terminate translation.

Question 3b:

i) What does it mean when someone says that a mutation is cis-acting?

ii) What would it mean if someone said that a mutation is trans-acting?

Question 2:

Translation is another name for protein synthesis. There are three steps that take place repeatedly during elongation.

a) Give the name and a short description of what happens during the first step of elongation during translation?

b) Give the name and a short description of what happens during the second step of elongation during translation?

C) Give the name and a short description of what happens during the third step of elongation during translation?

Please help! Thanks

What is allomothering and how is it manifested? Use no less than three examples (at least one human and one nonhuman)? What are the most important benefits attributed to social units that engage in allomothering? How might this information be useful in modern society?

Why do most human bodies tan when exposed to UV light? Explain the possible relationships between ultraviolet light, vitamin D, folic acid and melanin production with regard to the evolution of various skin colors in human population

DNA EXTRACTION & PURITY PROTOCOL

1. Add a small piece of peeled banana (2-3 cm) and 12mL extraction buffer (NaCl & Sodium Citrate) into the beaker. This buffer contains salts necessary for the precipitation of the DNA. The ions in the solution make it more polar facilitating the dissolving of the DNA.
2. Add 3mL detergent into the beaker. This will break open the cells and dissolve the DNA. Detergent breaks up the lipids of the plasma and nuclear membranes

Questions:

a. Why does a more polar solution make DNA more likely to dissolve?

b. How does detergent break up lipids in a membrane?

Imagine you identified what you believe to be a new, previously-unidentified spe- cies of bacterium. You therefore cultured the bacterium in the lab, purified RNA from the bacteria, made cDNA from the RNA, and obtained its sequence. What next step would you take to confirm whether this is, indeed, a novel species?