A new model of transgenic vole was created in which all females are spontaneously maternal. Which experimental manipulation would you expect to cause this result?

A) over expression of oxytocin in the ventral pallidum

B) over expression of D2 receptors in the caudate putamen

C) over expression of vasopressin receptors in the nucleus accumbens

D) over expression of oxytocin receptors in the nucleus accumbens

Question 1 (1 point)

Saved

Imagine that you are working with DNA sequences of soluble proteins (not membrane proteins) and have the technology to genetically engineer/alter the existing sequence and you can express this particular protein in a yeast cell model.

You also have the power to track and visualize where the genetically engineered proteins traffic in your model system.  

You engineer an ER signal sequence to the amino-terminal end of a normally cytosolic protein.   

Which best describes the fate of this protein?

Question 1 options:

Question 2 (1 point)

You engineer an ER signal sequence to the carboxyl-terminal end of a normally cytosolic protein.   

Which best describes the fate of this protein?

Question 2 options:

Your supervisor wants you to dilute a 1 mL stock BSA solution (1 mg/mL) into 1:10 dilutions. What is the final concentration of diluted BSA solution? Show your work. How much volume (mL) of BSA and distilled water would you need to perform the dilution? Show your work.

Question 20 (Mandatory) (1 point)

Select all of the following which could cause an anemia that is classified as normocytic/ normochromic.

Question 20 options:

Question 21 (Mandatory) (1 point)

Which of the following is NOT associated with a spectrin abnormality?

Question 21 options:

Question 22 (Mandatory) (1 point)

Stomatocytes can be seen in increased numbers on the slide due to poor slide making technique.

Question 22 options:

Question 23 (Mandatory) (1 point)

Abetalipoproteinemia will show which of the following on the peripheral blood smear?

Question 23 options:

Question 24 (Mandatory) (1 point)

A decrease in all cell lines is referred to as ______________________.

Question 24 options:

Question 25 (Mandatory) (1 point)

Rh null disease is associated with the presence of ____________________.

Question 25 options:

1.Imagine a locus with two alleles, A and a, in a wild population of touch-me-not (Impatiens capensis) plants,which has both out crossing and selfing flowers.40 AA, 2 Aa, and 18 aa genotypes are found among 60 individuals.

a.Calculate the allele frequencies p= freq(A) and q=freq(a)

.b.How many individuals of each genotype would be expected if the HW assumption were met?

c.Is this population in HWE? Explain your answer.

d.Uh oh,bad year for pollinators!What would be the genotype frequencies in the next generation if the plant only produced selfed progeny? (Hint: the coefficient of inbreeding for selfing in a diploid is 0.5.This is because the chance of the two alleles in the selfed offspring being identical-by-descent one generation back is 1/2).

e.Oh no, it looks like the pollinators are gone forever!What would be the frequency of heterozygotes at equilibrium (i.e. after many,many generations of selfing)

Why do evergreen plants of northern wetlands have many xerophytic features? Should this also be true for deciduous plants in these habitats?

How might you distinguish between a lymphocytosis from an infection versus one from a malignancy?

Your neighbors have been feeding the birds – a lot – and now they’ve got rats in their yard, happily feasting on the leftover birdseed. You don’t want the rats near your house, and having little regard for the life of vermin, you decide to place a little “deterrent” on your property. Nosing around in a lab, you find arsenic. OK, you are smart enough not to actually use it, but you can’t help but think about it...

If a rat ingests arsenic, the enzyme __________________________ will be inhibited, and the metabolic intermediate ___________________________ will NOT be converted to ___________________________ which is the key entry point to the energy-generating pathway known as the ___________________________. Instead, this metabolic intermediate will be shunted into the metabolic pathway known as ___________________________ which does not require the function of the inhibited enzyme. The glucose- derived end-product of this pathway is ___________________________ and the net energy yield from glucose is ___________________________ NADH and ___________________________ ATP.

Can you please write about DNA extraction from blood and preparation of blood samples for DNA extraction. This is the protocol:

1.Take 750 μl of blood from each blood sample, and transfer it to new 1.5ml tube;
2. Mix 750 μl of blood with 1ml RBC lysis buffer, respectively (avoid stock contamination by not touching the walls of tube by pipette tip);
3. Centrifuge at 2300 rpm for 5 minutes;
4. Take out the liquid, keep the pellet;
5. Mix 1 ml of RBC lysis buffer with pellet in 15ml centrifuge tube;
6. Add PBS up to 14 ml by pipette controller (increase volume and contrast);
7. Centrifuge at 2200 rpm, at 4°C, for 5 minutes
8. Discard the supernatant, keep the pellet;

9. Add 1 ml of RBC lysis buffer;
10. Add PBS up to 10 ml;
11. Centrifuge at 2200 rpm, at 4°C, for 5 minutes;
12. Discard the supernatant and resuspend the pellet in 1 ml of Trizol;
13. Store the samples at -20°C

Can you explain the protocol, why are we doing these steps. Also if you can say the function of each chemical:

• RBC lysis buffer
• PBS
• Trizol

Thank you in advance. I really really need your help.

1. Arrange the following molecules from least to most specific with respect to the original nucleotide sequence: RNA, DNA, Amino Acid, Protein.

2. Identify two structural differences between DNA and RNA.

3. Suppose you are performing an experiment in which you must use heat to denature a double helix and create two single stranded pieces. Based on what you know about nucleotide bonding, do you think the nucleotides will all denature at the same time? Use scientific reasoning to explain why.

EXPERIMENT 1: CODING

1. Using the red, blue, yellow and green beads, devise and lay out a three color code (or codon) for each of the following letters (codon). For example Z = green : red : green.

• C:

• E:

• H:

• I:

• K:

• L:

• M:

• O:

• S:

• T:

• U

• Start:

• Stop:

• Space:

PLEASE LABEL WITH NUMBERS ON SIDE/ ALSO TYPE ANSWERS AS I CANT READ HANDWRITTEN ANSWERS

Describe the experimental basis for the following fundamental principle about mutations:

“The mutational process is random in the sense that whether a particular mutation happens is unrelated to any adaptive advantage it may confer on the organism in its environment. A potentially favorable mutation does not arise because the organism has a need for it.”

What are some enzymatic challenges used by plants to evolve under increasing co2 concentrations and where does each carbon cycle pathway give benefit to the plant?

Write a 500 word essay on why eliminating carcinogens from the environment would be a more successful strategy than attempting to find a cure for cancer. You can organize the essay as if it were a rhetorical (argumentative) essay.  1) Introductory paragraph that includes your point or view, three reasons that you will argue (ordered from strong to strongest), and some background, 2) Paragraph 2: argument in favor of point 1 (strong point), 3) Paragraph 3: argument in favor of point 2 (stronger point), 4) Paragraph 4: argument in favor of point 3 (strongest point), and 5) Paragraph 5: summary of argument (powerful restatement of first paragraph).

Quarantines of different kinds are imposed in pandemics. Lockdowns, shelter in place, stay at home like legal orders are invoked by the governments and are implemented with the help of law enforcement agencies (if required). Such regulations create ethical issues of privacy & its violation. Please discuss all the above in the light of Coronavirus COVID-19 pandemic. Write in no less than 1 page & no more than 2 pages