Your supervisor wants you to dilute a 1 mL stock BSA solution (1 mg/mL) into 1:10...

Your supervisor wants you to dilute a 1 mL stock BSA solution (1 mg/mL) into 1:10 dilutions. What is the final concentration of diluted BSA solution? Show your work. How much volume (mL) of BSA and distilled water would you need to perform the dilution? Show your work.

Expert Solution

gladiator answered 4 months ago

You have 0.5 ml of a stock solution of 1 mg/ml BSA. You need at least...

You have 0.5 ml of a stock solution of 1 mg/ml BSA. You need at least 200 ul of the solutions listed below. Calculate the volume of BSA needed and the volume of water needed to make the solutions. 25 ug/ml 50 ug/ml 75 ug/ml 100 ug/ml 150 ug/ml 200 ug/ml

A) Given 37ul of a 100 mg/ml stock solution of Bovine Serum Albumin (BSA), diagram the...

A) Given 37ul of a 100 mg/ml stock solution of Bovine Serum Albumin (BSA), diagram the most accurate procedure so you end up with at least 500ul each of 5ug/ul, 0.5 ug/ul, and 0.05 ug/ul working solutions of BSA. B) Using the BSA working solutions created above, how much and which concentration would you pipet into three different centrifuge tubes so you end up with 20 ug, 2 ug, and 0.2 ug of BSA? (Note: remember to stay within the...

You make two dilutions. You take 82.19 mL of the stock solution (8.1×10-5M) and dilute to...

You make two dilutions. You take 82.19 mL of the stock solution (8.1×10-5M) and dilute to 500.0 mL to make Solution #1. You then add 66.26 mL of Solution #1 to 30.11 mL of water to make Solution #2. What is the concentration of the Solution #1 in terms of molarity? What is the concentration of the Solution #2 in terms of molarity?

you have 1-5 ml of 0.140 M HCl solution and you want to dilute it to...

you have 1-5 ml of 0.140 M HCl solution and you want to dilute it to exactly 0.100 M. How much water should you add?

1) You have 100 ml of a solution of ATP. You take 25 ?l, and dilute...

1) You have 100 ml of a solution of ATP. You take 25 ?l, and dilute it to a final volume of 1ml. The absorbance of the diluted solution at 259 nm is 0.550. a) What is the ATP concentration in the original, undiluted solution? **The molar extinction coefficient for ATP at 259 nm is 15,600 l mol-1cm-1. b. How many mg of ATP are there in total in the original, undiluted solution? **The formula weight of ATP is 551.14...

To what volume (in mL) should you dilute 100.0 mL of a 5.00 M CaC2 solution...

To what volume (in mL) should you dilute 100.0 mL of a 5.00 M CaC2 solution to obtain a 0.750 M CaC2 solution?

2) You have 25 ml of an ATP solution. You take 30 µl and dilute to...

2) You have 25 ml of an ATP solution. You take 30 µl and dilute to a final volume of 10 ml. The absorbance of this solution is 0.432. What is the concentration of the undiluted ATP solution? (molar extinction coefficient = 15,400 M-1cm-1). If you could upload image the solution you worked out on paper, that would be preferred.

To what volume (in milliliters) should you dilute 100.0 mL of a 4.50 M solution of...

To what volume (in milliliters) should you dilute 100.0 mL of a 4.50 M solution of CaCl2 solution to obtain a 0.900 M solution of CaCl2? Express the volume to three significant figures and include the appropriate units.

1. If you mix a 10 mL of a 0.1 solution of glucose with a 10...

1. If you mix a 10 mL of a 0.1 solution of glucose with a 10 mL of a 0.1 M solution of ethanol, how would you expect the conductance change? 2. How does the conductance of a strong electrolyte change as the concentration decreases? 3. How does the conductance of a weak electrolyte change as the concentration decreases?

Lab 5: Practice Dilution Problems You have a stock solution that contains 24 mg/ml of protein....

Lab 5: Practice Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 8 mg/ml 4 mg/ml 2 mg/ml You need a minimum of 300 ul of each standard. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Standard 1 Standard 2 Standard 3 concentration 8 mg/ml 4 mg/ml 2 mg/ml dilution...

Question