You are a microbiology lab technician and you need to quantify the viable cell concentration in your stock culture before beginning an experiment. Since you do not know the range, you perform a serial dilution over several orders of magnitude. You use the pour plate method and plate 1 ml for each dilution level, in duplicate. Give the most accurate value for the number of viable cells in the original stock culture, in CFU / ml. Just enter a number, do not type the units!
Since you want the practice, you attempt a count for all plates, even though some are likely too numerous to count. Only use values that give you the best accuracy. That means only use values that are a "countable number" of colonies (see text section).
Dilution series colony counts:
1:10: 860, 745
1:100: 123, 114
1:1000: 11, 15
1:10,000: 2, 4
In: Biology
Matching
|
1. |
Astrocytes |
A. Sheet-like cancer of epithelial origin |
|
2. |
Immortality |
B. Can cause senescence in dividing cells |
|
3. |
Squamous carcinoma |
C. Increases risk of breast cancer |
|
4. |
Metaplasia (example) |
D. Decreases risk of breast cancer |
|
5. |
Adenocarcinoma |
E. Associated with stomach cancer incidence patterns |
|
6. |
Having no natural-born children |
F. Can affect cell shape, cell adhesion, and cell motility |
|
7. |
Over-expression or mis-expression of a receptor on breast cancer cells |
G. Supporting cells in the brain |
|
8. |
aneuploidy |
H. Can cross-link receptors in the membrane of a cell |
|
9. |
Helicobacter pylori infection |
I. Neoplasm brain tissue origin |
|
10. |
Shortening of telomeres |
J. Neoplasm of striated muscle cell origin |
|
11. |
Leading cause of cancer deaths in women |
K. Can lead to activation of cancer-causing genes |
|
12. |
Astrocytoma or glioblastoma |
L. The lymphatic system |
|
13. |
cytoskeletal anaplasia |
M. Can have early onset multifocal incidence pattern |
|
14. |
Bilateral retinoblastoma |
N. Phenotypic characteristic of many cancer cells in culture |
|
15. |
Genomic instability and selection |
O. Hepatitis B Virus |
|
16. |
Onco-fetal gene products |
P. Sac-like or glandular neoplasm of epithelial cell origin |
|
17. |
Bivalent growth factors |
Q. Ewing's sarcoma |
|
18. |
Can lead to hepatocellular carcinoma |
R. Can lead to drug resistance during treatment |
|
19. |
Genetic drift |
S. Ciliated epithelium replaced by squamous epithelium |
|
20. |
Lower dietary fat and body mass |
T. Abnormal number and form of chromosomes |
|
21. |
Rhabdomyosarcoma |
U. Can happen when cell lines are passaged repeatedly |
|
22. |
Fusion protein gene product |
V. Often re-expressed in cancer cells |
|
23. |
Protease inhibitors |
W. Target for Herceptin |
|
24. |
Route of metastatic spread |
X. Lung cancer |
|
25. |
form of bone cancer |
Y. Might inhibit invasion |
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| Region | Function | Protein |
| Dendrite | ||
| Soma | ||
| Axon | ||
| Axon terminal |
Complete the table above on the regions of the neuron, their primary function(s), and a specific protein that supports that function. Explain your choice of protein.
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Define colony collapse disorder. and why we should be concerned. If you do not think this is a concern state your evidence for this opinion as well.
Are you surprised by the economic impact of colony collapse disorder?
If pollinators are eliminated in an ecosystem how will this affect the genetic diversity of the population?
What should or could be done to limit the effect of or help eliminate this problem?
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I have some questions that I need answe for them
2) Briefly describe the absolute refractory period in relation to V-gated sodium channels, V-gated potassium channels, and the membrane permeability to those ions.
3) When you determined the relative refractory period, you actually recorded the interval when a few neurons entered the relative refractory period. Explain why this interval signifies the beginning of the relative refractory period and not the absolute refractory period.
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