Say you design a PCR experiment with normal temperature settings and reagent amounts (primers, dNTPs, polymerase) for 20 cycles. You want to adjust your experiment so that it will increase the amount of DNA produced. Which of the following adjustments to the experiment will increase the amount of DNA amplified during PCR?
Group of answer choices
You increase the length of the primers.
You increase the temperatures in all of the steps.
None of these adjustments will increase the amount of DNA produced through PCR.
You run 5 extra cycles.
You double the amount of dNTPs.
In: Biology
what is the missing part of problems resulting from excessive consumption of protein
In: Biology
5. FA monomers indirectly interact via covalent bonds with other molecules to form lipid/fat polymers. Explain what this means, and why an additional molecule must be involved. Also describe the interaction that does occurs between FA tails, and why this interaction occurs; compare these interactions in saturated and unsaturated FAs.
In: Biology
Mitochondria are unable to survive outside of eukaryotic cells. If they originated from ancestral prokaryotes, as proposed by the endosymbiotic theory, then why can’t they survive independently?
In: Biology
Design a project that will show information about photosynthesis
and cellular respiration and the connections between the two. Terms
to Include in the Project: (these terms must be in the
project)
Carbon Dioxide (CO2), Oxygen (O2), Glucose (C6H12O6),
Sun/Sunlight/Light, Water (H20), ATP, chloroplast,
mitochondria
Concepts to Include: Function of both processes, location of both
process, reactants of both, products of both, how the two processes
are related in terms of the light dependent, light independent,
glycolysis, citric acid cycle, electron transport chain, what is
ATP and how does it do its job.
In: Biology
why do we use % change in weight vs difference in weight
In: Biology
Chemical potential energy vs. kinetic energy
Know the difference between these two energy types.
Energy Transformation
Relate and define
Laws of Thermodynamics
What are the two laws of thermodynamics?
Endergonic Reactions- define
Exergonic reactions – define
Enzymes
What are they?
How do they work?
What is activation energy?
What is an active site?
How do you denature an enzyme?
Cellular Respiration Review
Cellular respiration and energy conversion
What does cellular respiration do in relation to energy conversion?
Oxidation-Reduction reaction (redox reactions)
What is a redox reaction?
In: Biology
1. The following questions concern Jesse Gelsinger who suffered from partial OTC.
a. In the United States the average person eats far more protein than needed to meet the demands of protein synthesis in their cells. What happens to the extra amino acids? Be specific.
b. Describe Jesse’s illness.
c. Briefly describe the experimental treatment he underwent.
In: Biology
All bacteria are able to use citrate for energy or as a carbon source. That being said, only some bacteria can grow on citrate media. Explain why bacteria that can use citrate for energy and carbon may not be able to grow on this media.
In: Biology
Describe the appearance of organisms observed under the microscope after staining a bacterial smear on a slide using a positive staining technique and the appearance of organisms observed after staining a bacterial smear on a slide with a negative staining technique. which type of dye is used in each technique?
In: Biology
In: Biology
4) Write the mathematical expression for water potential (includes solute and pressure potential) and give a brief definition for each variable.
In: Biology
Can DNA be amplified from a degraded sample for PCR?
In: Biology
Written Report Format: Introduction, body, conclusion, and bibliography
Learn about Archaea:
In a written report form tell me everything about the bad (diseases) and good, they cause in the world.
In: Biology
Description of cystic fibrosis?
What has changed in cystic fibrosis to allow it to cause a genetic disorder? Explain how this differs from the gene's normal function.Is it possible for parents to not have the disorder, but pass it on to their children?
What kind of dominance pattern does cystic fibrosis show?
What problem(s) is/are caused to a person who inherits the cystic fibrosis gene? Is this typically inherited from the mother or the father?
Describe an experiment that you could use to determine if a new drug is effective at treating cystic fibrosis. Be sure to mention the independent, dependent and control variables.
How you could use the scientific method to draw conclusions about cystic fibrosis or to treat this disorder?
In: Biology