Questions
Say you design a PCR experiment with normal temperature settings and reagent amounts (primers, dNTPs, polymerase)...

Say you design a PCR experiment with normal temperature settings and reagent amounts (primers, dNTPs, polymerase) for 20 cycles. You want to adjust your experiment so that it will increase the amount of DNA produced. Which of the following adjustments to the experiment will increase the amount of DNA amplified during PCR?

Group of answer choices

You increase the length of the primers.

You increase the temperatures in all of the steps.

None of these adjustments will increase the amount of DNA produced through PCR.

You run 5 extra cycles.

You double the amount of dNTPs.

In: Biology

what is the missing part of problems resulting from excessive consumption of protein

what is the missing part of problems resulting from excessive consumption of protein

In: Biology

5. FA monomers indirectly interact via covalent bonds with other molecules to form lipid/fat polymers. Explain...

5. FA monomers indirectly interact via covalent bonds with other molecules to form lipid/fat polymers. Explain what this means, and why an additional molecule must be involved. Also describe the interaction that does occurs between FA tails, and why this interaction occurs; compare these interactions in saturated and unsaturated FAs.

In: Biology

Mitochondria are unable to survive outside of eukaryotic cells. If they originated from ancestral prokaryotes, as...

Mitochondria are unable to survive outside of eukaryotic cells. If they originated from ancestral prokaryotes, as proposed by the endosymbiotic theory, then why can’t they survive independently?

In: Biology

Design a project that will show information about photosynthesis and cellular respiration and the connections between...

Design a project that will show information about photosynthesis and cellular respiration and the connections between the two. Terms to Include in the Project: (these terms must be in the project)
Carbon Dioxide (CO2), Oxygen (O2), Glucose (C6H12O6), Sun/Sunlight/Light, Water (H20), ATP, chloroplast, mitochondria
Concepts to Include: Function of both processes, location of both process, reactants of both, products of both, how the two processes are related in terms of the light dependent, light independent, glycolysis, citric acid cycle, electron transport chain, what is ATP and how does it do its job.

In: Biology

why do we use % change in weight vs difference in weight

why do we use % change in weight vs difference in weight

In: Biology

Chemical potential energy vs. kinetic energy             Know the difference between these two energy types. Energy...

Chemical potential energy vs. kinetic energy

            Know the difference between these two energy types.

Energy Transformation

            Relate and define

Laws of Thermodynamics

            What are the two laws of thermodynamics?

Endergonic Reactions- define

Exergonic reactions – define

Enzymes

            What are they?

            How do they work?

What is activation energy?

            What is an active site?

            How do you denature an enzyme?

Cellular Respiration Review

Cellular respiration and energy conversion

            What does cellular respiration do in relation to energy conversion?

           

Oxidation-Reduction reaction (redox reactions)

            What is a redox reaction?

In: Biology

1. The following questions concern Jesse Gelsinger who suffered from partial OTC.             a. In the...

1. The following questions concern Jesse Gelsinger who suffered from partial OTC.

            a. In the United States the average person eats far more protein than needed to meet the demands of protein synthesis in their cells. What happens to the extra amino acids? Be specific.

            b. Describe Jesse’s illness.

            c. Briefly describe the experimental treatment he underwent.

In: Biology

All bacteria are able to use citrate for energy or as a carbon source. That being...

All bacteria are able to use citrate for energy or as a carbon source. That being said, only some bacteria can grow on citrate media. Explain why bacteria that can use citrate for energy and carbon may not be able to grow on this media.

In: Biology

Describe the appearance of organisms observed under the microscope after staining a bacterial smear on a...

Describe the appearance of organisms observed under the microscope after staining a bacterial smear on a slide using a positive staining technique and the appearance of organisms observed after staining a bacterial smear on a slide with a negative staining technique. which type of dye is used in each technique?

In: Biology

i want the answer for these 3 question 1 -Describe the classification of furcation lesions and...

i want the answer for these 3 question

1 -Describe the classification of furcation lesions and relate these to clinical and radiographic findings.
2 -Discuss the differential diagnosis of a radiograph demonstrating a lesion in the inter-radicular space of a multi-rooted tooth.
3-Explain the treatment objectives and the treatment methods for managing furcation lesion

In: Biology

4) Write the mathematical expression for water potential (includes solute and pressure potential) and give a...

4) Write the mathematical expression for water potential (includes solute and pressure potential) and give a brief definition for each variable.

In: Biology

Can DNA be amplified from a degraded sample for PCR?

Can DNA be amplified from a degraded sample for PCR?

In: Biology

Written Report Format: Introduction, body, conclusion, and bibliography Learn about Archaea: In a written report form...

Written Report Format: Introduction, body, conclusion, and bibliography

Learn about Archaea:

In a written report form tell me everything about the bad (diseases) and good, they cause in the world.

In: Biology

Description of cystic fibrosis? What has changed in cystic fibrosis to allow it to cause a...

Description of cystic fibrosis?

What has changed in cystic fibrosis to allow it to cause a genetic disorder? Explain how this differs from the gene's normal function.Is it possible for parents to not have the disorder, but pass it on to their children?

What kind of dominance pattern does cystic fibrosis show?

What problem(s) is/are caused to a person who inherits the cystic fibrosis gene? Is this typically inherited from the mother or the father?

Describe an experiment that you could use to determine if a new drug is effective at treating cystic fibrosis. Be sure to mention the independent, dependent and control variables.

How you could use the scientific method to draw conclusions about cystic fibrosis or to treat this disorder?

In: Biology