pipette calibiration procedure

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queen_honey_blossom answered 2 years ago

In a lab you are asked to pipette 130 microL using a pipette with a range...

In a lab you are asked to pipette 130 microL using a pipette with a range of 20-200 microL. This pipette has recently been calibrated so is functioning correctly. You correctly move the dial of the pipette to 130 microL. On your first attempt to dispense 130 microL, you realise that the volume expelled was only 105 microL. On the second occasion it was 170 microL. Give explanations to what operator errors could have led to these outcomes?

-Why is it important to use a seperate serological pipette for media and cell solutions? -What...

-Why is it important to use a seperate serological pipette for media and cell solutions? -What is the purpose of using Trypsin EDTA and what does EDTA and trypsin do specifically?

In the Fluorometric analysis of Quinine in Tonic Water Lab Pipette 50 μL of the tonic...

In the Fluorometric analysis of Quinine in Tonic Water Lab Pipette 50 μL of the tonic water into the 10 mL flask and dilute to 10.00 mL with 0.05 M H2SO4. This will be your first sample for analysis. Prepare a second sample by diluting the first solution by a factor of 2. You can do this by pipetting 1.00 mL of solution the first solution and 1.00 mL of 0.05 M H2SO4 into a vial. From the platereader (fluorescence...

In microscale extractions you are instructed to remove the lower phase using a pastuer pipette even...

In microscale extractions you are instructed to remove the lower phase using a pastuer pipette even when the organic phase is the top phase. Explain why this results in better phase separation than removing the top phase directly with the pastuer pipette

write instructions how to pipette 5 microliters of fluid AND draw a diagram that shows how...

write instructions how to pipette 5 microliters of fluid AND draw a diagram that shows how to complete the serial dilutions to conduct. Diagram of a Serial Dilution (use P-1000 set to 5,0,0)

List 4 safety features that you will need to follow when using the pipette (In microbiology).

What does it mean if there is air at the narrow end of the Pipette tip when doing an experiment

In the experiement, each flask containing a water sample, pipette 10.00 mL of the AgNO3 solution....

In the experiement, each flask containing a water sample, pipette 10.00 mL of the AgNO3 solution. Using a small graduated cylinder, carefully add 3.0 mL of Fe(NO3)3 solution and 2.0 mL of 6M HNO3 to each water sample. the trial is now ready for titration. Obtain approximately 120 mL of stock KSCN solution. Rinse out a clean buret with about 10 mL of KSCN at least twice, and discard the washing solution into a waste beaker. From the calculation , number...

Using a pipette 30 μL of “3.3× strength isosensitised broth” was added to each well to...

Using a pipette 30 μL of “3.3× strength isosensitised broth” was added to each well to ensure that the final volume was single strength of the nutrient broth， what mean about “3.3× strength isosensitised broth”，how to prepare it？？and what mean about single strength of the nutrient broth？？？？

What is the diagnostic procedure for glucose tolerance? ACTIVE LEARNING TEMPLATE:Diagnostic Procedure STUDENT NAME _____________________________________ PROCEDURE...

What is the diagnostic procedure for glucose tolerance? ACTIVE LEARNING TEMPLATE:Diagnostic Procedure STUDENT NAME _____________________________________ PROCEDURE NAME ____________________________________________________________________ REVIEW MODULE CHAPTER ___________ Description of Procedure Indications Interpretation of Findings. CONSIDERATIONS Nursing Interventions (pre, intra, post) Potential Complications. Client Education Nursing Interventions. ACTIVE LEARNING TEMPLATES TherapeuTic procedure A3

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